Characterization, localization and comparison of c-Kit+ lung cells in never smokers and smokers with and without COPD

Background: c-Kit + lung stem cells have been described in the human healthy lung. Their potential relation with smoking and/or chronic obstructive pulmonary disease (COPD) is unknown. Methods: We characterized and compared c-Kit+ cells in lung tissue of 12 never smokers (NS), 15 smokers with normal...

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Autores: López Giraldo, Alejandra, Cruz, Tamara, Molins López-Rodó, Laureano, Guirao Montes, Àngela, Saco, Adela, Cuerpo Cardeñosa, Sandra, Ramírez Ruz, J. (José), Agustí García-Navarro, Àlvar, Faner, Rosa
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/132850
Acesso em linha:https://hdl.handle.net/2445/132850
Access Level:acceso abierto
Palavra-chave:Malalties pulmonars obstructives cròniques
Pulmó
Fumadors
Bronquitis
Chronic obstructive pulmonary diseases
Lung
Cigarette smokers
Bronchitis
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spelling Characterization, localization and comparison of c-Kit+ lung cells in never smokers and smokers with and without COPDLópez Giraldo, AlejandraCruz, TamaraMolins López-Rodó, LaureanoGuirao Montes, ÀngelaSaco, AdelaCuerpo Cardeñosa, SandraRamírez Ruz, J. (José)Agustí García-Navarro, ÀlvarFaner, RosaMalalties pulmonars obstructives cròniquesPulmóFumadorsBronquitisChronic obstructive pulmonary diseasesLungCigarette smokersBronchitisBackground: c-Kit + lung stem cells have been described in the human healthy lung. Their potential relation with smoking and/or chronic obstructive pulmonary disease (COPD) is unknown. Methods: We characterized and compared c-Kit+ cells in lung tissue of 12 never smokers (NS), 15 smokers with normal spirometry (S) and 44 COPD patients who required lung resectional surgery. Flow cytometry (FACS) was used to characterize c-Kit+ cells in fresh lung tissue disaggregates, and immunofluorescence (IF) for further characterization and to determine their location in OCT- embedded lung tissue. Results: We identified 4 c-Kit+ cell populations, with similar proportions in NS, S and COPD: (1) By FACS, c-Kithigh/CD45 + cells (4.03 ± 2.97% (NS), 3.96 ± 5.30% (S), and 5.20 ± 3.44% (COPD)). By IF, these cells were tryptase+ (hence, mast cells) and located around the airways; (2) By IF, c-Kitlow/CD45+/triptase- (0.07 ± 0.06 (NS), 0.03 ± 0.02 (S), and 0.06 ± 0.07 (COPD) cells/field), which likely correspond to innate lymphoid cells; (3) By FACS, c-Kitlow/CD45-/CD34+ (0.95 ± 0.84% (NS), 1.14 ± 0.94% (S) and 0.95 ± 1.38% (COPD)). By IF these cells were c-Kitlow/CD45-/CD31+, suggesting an endothelial lineage, and were predominantly located in the alveolar wall; and, (4) by FACS, an infrequent c-Kitlow/CD45-/ CD34- population (0.09 ± 0.14% (NS), 0.08 ± 0.09% (S) and 0.08 ± 0.11% (COPD)) compatible with a putative lung stem cell population. Yet, IF failed to detect them and we could not isolate or grow them, thus questioning the existence of c-Kit+ lung stem-cells. Conclusions: The adult human lung contains a mixture of c-Kit+ cells, unlikely to be lung stem cells, which are independent of smoking status and/or presence of COPD.BioMed Central2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/132850Articles publicats en revistes (Fonaments Clínics)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1186/s12890-018-0688-3BMC Pulmonary Medicine, 2018, vol. 18, p. 123https://doi.org/10.1186/s12890-018-0688-3cc-by (c) López Giraldo, Alejandra et al., 2018http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1328502026-05-27T06:46:51Z
dc.title.none.fl_str_mv Characterization, localization and comparison of c-Kit+ lung cells in never smokers and smokers with and without COPD
title Characterization, localization and comparison of c-Kit+ lung cells in never smokers and smokers with and without COPD
spellingShingle Characterization, localization and comparison of c-Kit+ lung cells in never smokers and smokers with and without COPD
López Giraldo, Alejandra
Malalties pulmonars obstructives cròniques
Pulmó
Fumadors
Bronquitis
Chronic obstructive pulmonary diseases
Lung
Cigarette smokers
Bronchitis
title_short Characterization, localization and comparison of c-Kit+ lung cells in never smokers and smokers with and without COPD
title_full Characterization, localization and comparison of c-Kit+ lung cells in never smokers and smokers with and without COPD
title_fullStr Characterization, localization and comparison of c-Kit+ lung cells in never smokers and smokers with and without COPD
title_full_unstemmed Characterization, localization and comparison of c-Kit+ lung cells in never smokers and smokers with and without COPD
title_sort Characterization, localization and comparison of c-Kit+ lung cells in never smokers and smokers with and without COPD
dc.creator.none.fl_str_mv López Giraldo, Alejandra
Cruz, Tamara
Molins López-Rodó, Laureano
Guirao Montes, Àngela
Saco, Adela
Cuerpo Cardeñosa, Sandra
Ramírez Ruz, J. (José)
Agustí García-Navarro, Àlvar
Faner, Rosa
author López Giraldo, Alejandra
author_facet López Giraldo, Alejandra
Cruz, Tamara
Molins López-Rodó, Laureano
Guirao Montes, Àngela
Saco, Adela
Cuerpo Cardeñosa, Sandra
Ramírez Ruz, J. (José)
Agustí García-Navarro, Àlvar
Faner, Rosa
author_role author
author2 Cruz, Tamara
Molins López-Rodó, Laureano
Guirao Montes, Àngela
Saco, Adela
Cuerpo Cardeñosa, Sandra
Ramírez Ruz, J. (José)
Agustí García-Navarro, Àlvar
Faner, Rosa
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Malalties pulmonars obstructives cròniques
Pulmó
Fumadors
Bronquitis
Chronic obstructive pulmonary diseases
Lung
Cigarette smokers
Bronchitis
topic Malalties pulmonars obstructives cròniques
Pulmó
Fumadors
Bronquitis
Chronic obstructive pulmonary diseases
Lung
Cigarette smokers
Bronchitis
description Background: c-Kit + lung stem cells have been described in the human healthy lung. Their potential relation with smoking and/or chronic obstructive pulmonary disease (COPD) is unknown. Methods: We characterized and compared c-Kit+ cells in lung tissue of 12 never smokers (NS), 15 smokers with normal spirometry (S) and 44 COPD patients who required lung resectional surgery. Flow cytometry (FACS) was used to characterize c-Kit+ cells in fresh lung tissue disaggregates, and immunofluorescence (IF) for further characterization and to determine their location in OCT- embedded lung tissue. Results: We identified 4 c-Kit+ cell populations, with similar proportions in NS, S and COPD: (1) By FACS, c-Kithigh/CD45 + cells (4.03 ± 2.97% (NS), 3.96 ± 5.30% (S), and 5.20 ± 3.44% (COPD)). By IF, these cells were tryptase+ (hence, mast cells) and located around the airways; (2) By IF, c-Kitlow/CD45+/triptase- (0.07 ± 0.06 (NS), 0.03 ± 0.02 (S), and 0.06 ± 0.07 (COPD) cells/field), which likely correspond to innate lymphoid cells; (3) By FACS, c-Kitlow/CD45-/CD34+ (0.95 ± 0.84% (NS), 1.14 ± 0.94% (S) and 0.95 ± 1.38% (COPD)). By IF these cells were c-Kitlow/CD45-/CD31+, suggesting an endothelial lineage, and were predominantly located in the alveolar wall; and, (4) by FACS, an infrequent c-Kitlow/CD45-/ CD34- population (0.09 ± 0.14% (NS), 0.08 ± 0.09% (S) and 0.08 ± 0.11% (COPD)) compatible with a putative lung stem cell population. Yet, IF failed to detect them and we could not isolate or grow them, thus questioning the existence of c-Kit+ lung stem-cells. Conclusions: The adult human lung contains a mixture of c-Kit+ cells, unlikely to be lung stem cells, which are independent of smoking status and/or presence of COPD.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/132850
url https://hdl.handle.net/2445/132850
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1186/s12890-018-0688-3
BMC Pulmonary Medicine, 2018, vol. 18, p. 123
https://doi.org/10.1186/s12890-018-0688-3
dc.rights.none.fl_str_mv cc-by (c) López Giraldo, Alejandra et al., 2018
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) López Giraldo, Alejandra et al., 2018
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Fonaments Clínics)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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