Genome Editing by CRISPR/Cas9 in Polyploids

CRISPR/Cas system has been widely used for genome editing in the past few years. Even though it has been performed in many polyploid species to date, its efficient accomplishment in these organisms is still a challenge. The presence of multiple homoeologous genes as targets for their editing require...

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Detalles Bibliográficos
Autores: Sánchez-Gómez, Carlos, Posé, David, Martín-Pizarro, Carmen
Tipo de recurso: otro
Estado:Versión aceptada para publicación
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/372213
Acceso en línea:http://hdl.handle.net/10261/372213
https://api.elsevier.com/content/abstract/scopus_id/85147186818
Access Level:acceso abierto
Palabra clave:CRISPR/Cas9
Genome editing
Homoeologous genes
Off-targets
Polyploids
sgRNA
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spelling Genome Editing by CRISPR/Cas9 in PolyploidsSánchez-Gómez, CarlosPosé, DavidMartín-Pizarro, CarmenCRISPR/Cas9Genome editingHomoeologous genesOff-targetsPolyploidssgRNACRISPR/Cas system has been widely used for genome editing in the past few years. Even though it has been performed in many polyploid species to date, its efficient accomplishment in these organisms is still a challenge. The presence of multiple homoeologous genes as targets for their editing requires more rigorous work and specific needs to assess successful genome editing. Here, we describe a general stepwise protocol to select target sites, design sgRNAs, indicate vector requirements, and screen CRISPR/Cas9-mediated genome editing in polyploid species.CSG was supported by a grant from the Spanish Ministries of Science and Innovation (MICINN, RTI2018-09309-A-I00). CMP was supported by a grant from the European Research Council (ERC-2014-StG 638134).Peer reviewedSpringer NatureMinisterio de Ciencia e Innovación (España)European Research CouncilConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/otherhttp://purl.org/coar/resource_type/c_3248Postprintinfo:eu-repo/semantics/acceptedVersioninfo:eu-repo/semantics/bookParthttp://hdl.handle.net/10261/372213https://api.elsevier.com/content/abstract/scopus_id/85147186818reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//RTI2018-09309-A-I00info:eu-repo/grantAgreement/EC/H2020/638134Methods in molecular biology (Clifton, N.J.)https://doi.org/10.1007/978-1-0716-2561-3_24Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3722132026-05-22T06:33:51Z
dc.title.none.fl_str_mv Genome Editing by CRISPR/Cas9 in Polyploids
title Genome Editing by CRISPR/Cas9 in Polyploids
spellingShingle Genome Editing by CRISPR/Cas9 in Polyploids
Sánchez-Gómez, Carlos
CRISPR/Cas9
Genome editing
Homoeologous genes
Off-targets
Polyploids
sgRNA
title_short Genome Editing by CRISPR/Cas9 in Polyploids
title_full Genome Editing by CRISPR/Cas9 in Polyploids
title_fullStr Genome Editing by CRISPR/Cas9 in Polyploids
title_full_unstemmed Genome Editing by CRISPR/Cas9 in Polyploids
title_sort Genome Editing by CRISPR/Cas9 in Polyploids
dc.creator.none.fl_str_mv Sánchez-Gómez, Carlos
Posé, David
Martín-Pizarro, Carmen
author Sánchez-Gómez, Carlos
author_facet Sánchez-Gómez, Carlos
Posé, David
Martín-Pizarro, Carmen
author_role author
author2 Posé, David
Martín-Pizarro, Carmen
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
European Research Council
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv CRISPR/Cas9
Genome editing
Homoeologous genes
Off-targets
Polyploids
sgRNA
topic CRISPR/Cas9
Genome editing
Homoeologous genes
Off-targets
Polyploids
sgRNA
description CRISPR/Cas system has been widely used for genome editing in the past few years. Even though it has been performed in many polyploid species to date, its efficient accomplishment in these organisms is still a challenge. The presence of multiple homoeologous genes as targets for their editing requires more rigorous work and specific needs to assess successful genome editing. Here, we describe a general stepwise protocol to select target sites, design sgRNAs, indicate vector requirements, and screen CRISPR/Cas9-mediated genome editing in polyploid species.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/other
http://purl.org/coar/resource_type/c_3248
Postprint
info:eu-repo/semantics/acceptedVersion
dc.type.openaire.fl_str_mv info:eu-repo/semantics/bookPart
format other
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/372213
https://api.elsevier.com/content/abstract/scopus_id/85147186818
url http://hdl.handle.net/10261/372213
https://api.elsevier.com/content/abstract/scopus_id/85147186818
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI//RTI2018-09309-A-I00
info:eu-repo/grantAgreement/EC/H2020/638134
Methods in molecular biology (Clifton, N.J.)
https://doi.org/10.1007/978-1-0716-2561-3_24
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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