Synthesis and evaluation of novel furanones as biofilm inhibitors in opportunistic human pathogens

Diseases caused by biofilm-forming pathogens are becoming increasingly prevalent and represent a major threat to human health. This trend has prompted a search for novel inhibitors of microbial biofilms which could, for example, be used to potentiate existing antibiotics. Naturally-occurring, haloge...

Descripción completa

Detalles Bibliográficos
Autores: Gómez Camacho, Andromeda Celeste|||0000-0002-0075-6903, Lyons, Thérèse, Mamat, Uwe|||0009-0004-4919-8182, Yero, Daniel|||0000-0002-6234-4082, Bravo, Marc|||0000-0002-3173-2242, Daura i Ribera, Xavier|||0000-0001-9235-6730, Elshafee, Osama, Brunke, Sascha, Gahan, Cormac G.M., O'Driscoll, Michelle, Gibert, Isidre|||0000-0003-1442-2258, O'Sullivan, Timothy P.
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:270489
Acceso en línea:https://ddd.uab.cat/record/270489
https://dx.doi.org/urn:doi:10.1016/j.ejmech.2022.114678
Access Level:acceso abierto
Palabra clave:Furanones
Biofilms
Quorum sensing
Salmonella enterica
Staphylococcus aureus
Pseudomonas aeruginosa
Escherichia coli
Stenotrophomonas maltophilia
Candida albicans
Descripción
Sumario:Diseases caused by biofilm-forming pathogens are becoming increasingly prevalent and represent a major threat to human health. This trend has prompted a search for novel inhibitors of microbial biofilms which could, for example, be used to potentiate existing antibiotics. Naturally-occurring, halogenated furanones isolated from marine algae have proven to be effective biofilm inhibitors in several bacterial species. In this work, we report the synthesis of a library of novel furanones and their subsequent evaluation as biofilm inhibitors in several opportunistic human pathogens including S. enterica, S. aureus, E. coli, S. maltophilia, P. aeruginosa and C. albicans. A number of the most potent compounds were subjected to further analysis by confocal laser-scanning microscopy for their effects on P. aeruginosa and C. albicans biofilms individually, in addition to mixed polymicrobial biofilms. Lastly, we investigated the impact of a promising candidate on survival rates in vivo using a Galleria mellonella model.