Detection of clinically relevant variants in the TP53 gene below 10% allelic frequency: A multicenter study by ERIC, the European Research Initiative on CLL

In chronic lymphocytic leukemia, the reliability of next-generation sequencing (NGS) to detect TP53 variants ≤10% allelic frequency (low-VAF) is debated. We tested the ability to detect 23 such variants in 41 different laboratories using their NGS method of choice. The sensitivity was 85.6%, 94.5%,...

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Detalhes bibliográficos
Autores: Pavlova, Sarka, Malcikova, Jitka, Radova, Lenka, Bonfiglio, Silvia, Cowland, Jack B., Brieghel, Christian, Andersen, Mette K., Karypidou, Maria, Biderman, Bella, Doubek, Michael, Lazarian, Gregory, Rapado, Inmaculada, Vynck, Matthijs, Porret, Naomi A., Andres, Martin, Rosenberg, Dina, Sahar, Dvora, Martínez-Laperche, Carolina, Buño, Ismael, Hindley, Andrew, Donaldson, David, Sánchez, Julio B, García-Marco, José A., Serrano-Alcalá, Alicia, Ferrer-Lores, Blanca, Fernández-Rodriguez, Concepción, Bellosillo, Beatriz, Stilgenbauer, Stephan, Tausch, Eugen, Nikdin, Hero, Quinn, Fiona, Atkinson, Emer, Van de Corput, Lisette, Yildiz, Cafer, Bilbao-Sieyro, Cristina, Florido, Yanira, Thiede, Christian, Schuster, Caroline, Stoj, Anastazja, Czekalska, Sylwia, Chatzidimitriou, Anastasia, Laidou, Stamatia, Bidet, Audrey, Dussiau, Charles, Nollet, Friedel, Piras, Giovanna, Monne, Maria, Smirnova, Svetlana, Nikitin, Eugene, Sloma, Ivan, Claudel, Alexis, Largeaud, Laetitia, Ysebaert, Loïc, Valk, Peter J. M., Christian, Amy, Walewska, Renata, Oscier, David, Sebastião, Marta, Silva, Maria Gomes da, Galieni, Piero, Angelini, Mario, Rossi, Davide, Spina, Valeria, Matos, Sónia, Martins, Vânia, Stokłosa, Tomasz, Pepek, Monika, Baliakas, Panagiotis, Andreu, Rafa, Luna, Irene, Kahre, Tiina, Murumets, Ülle, Pikousova, Tereza, Kurucova, Terezia, Laird, Sophie, Ward, Daniel, Alcoceba, Miguel, Balanzategui, Ana, Scarfò, Lydia, Gandini, Francesca, Zapparoli, Ettore, Blanco, Adoración, Abrisqueta, Pau, Rodríguez-Vicente, Ana Eugenia, Benito, Rocío, Bravetti, Clotilde, Davi, Frédéric, Gameiro, Paula, Martínez-López, Joaquín, Tazón-Vega, Bárbara, Baran-Marszak, Fanny, Davis, Zadie, Catherwood, Mark, Sudarikov, Andrey, Rosenquist, Richard, Niemann, Carsten U., Stamatopoulos, Kostas, Ghia, Paolo, Pospisilova, Sarka
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/423775
Acesso em linha:http://hdl.handle.net/10261/423775
https://api.elsevier.com/content/abstract/scopus_id/85215519292
Access Level:acceso abierto
Descrição
Resumo:In chronic lymphocytic leukemia, the reliability of next-generation sequencing (NGS) to detect TP53 variants ≤10% allelic frequency (low-VAF) is debated. We tested the ability to detect 23 such variants in 41 different laboratories using their NGS method of choice. The sensitivity was 85.6%, 94.5%, and 94.8% at 1%, 2%, and 3% VAF cut-off, respectively. While only one false positive (FP) result was reported at >2% VAF, it was more challenging to distinguish true variants <2% VAF from background noise (37 FPs reported by 9 laboratories). The impact of low-VAF variants on time-to-second-treatment (TTST) and overall survival (OS) was investigated in a series of 1092 patients. Among patients not treated with targeted agents, patients with low-VAF TP53 variants had shorter TTST and OS versus wt-TP53 patients, and the relative risk of second-line treatment or death increased continuously with increasing VAF. Targeted therapy in ≥2 line diminished the difference in OS between patients with low-VAF TP53 variants and wt-TP53 patients, while patients with high-VAF TP53 variants had inferior OS compared to wild type-TP53 cases. Altogether, NGS-based approaches are technically capable of detecting low-VAF variants. No strict threshold can be suggested from a technical standpoint, laboratories reporting TP53 mutations should participate in a standardized validation set-up. Finally, whereas low-VAF variants affected outcomes in patients receiving chemoimmunotherapy, their impact on those treated with novel therapies remains undetermined. Our results pave the way for the harmonized and accurate TP53 assessment, which is indispensable for elucidating the role of TP53 mutations in targeted treatment.

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