MALDI-TOF mass spectrometry as a tool for differentiation of Bradyrhizobium species: Application to the identification of Lupinus nodulating strains

[EN]Genus Bradyrhizobium includes slow growing bacteria able to nodulate different legumes as well as species isolated from plant tumours. The slow growth presented by the members of this genus and the phylogenetic closeness of most of its species difficults their identification. In the present work...

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Bibliographic Details
Authors: Sánchez Juanes, Fernando, Ferreira, Laura, Alonso de la Vega, Pablo, Valverde, Angel, León Barrios, Milagros, Rivas González, Raúl, Mateos González, Pedro Francisco, Martínez Molina, Eustoquio, González de Buitrago Arriero, José Manuel, Trujillo Toledo, Martha Estela, Velázquez Pérez, María Encarnación
Format: article
Status:Published version
Publication Date:2013
Country:España
Institution:Universidad de Salamanca (USAL)
Repository:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/155199
Online Access:http://hdl.handle.net/10366/155199
Access Level:Embargoed access
Keyword:Bradyrhizobium
Identification
Lupinus
MALDI-TOF MS
Rhizobia
Cluster Analysis
Phylogeny
Sequence Analysis
Bacteriological Techniques
técnicas bacteriológicas
filogenia
análisis por grupos
análisis de secuencias
Description
Summary:[EN]Genus Bradyrhizobium includes slow growing bacteria able to nodulate different legumes as well as species isolated from plant tumours. The slow growth presented by the members of this genus and the phylogenetic closeness of most of its species difficults their identification. In the present work we applied for the first time Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) to the analysis of Bradyrhizobium species after the extension of MALDI Biotyper 2.0 database with the currently valid species of this genus. With this methodology it was possible to identify strains belonging to phylogenetically closely related species of genus Bradyrhizobium allowing the discrimination among species with rrs gene identities higher than 99%. The application of MALDI-TOF MS to strains isolated from nodules of different Lupinus species in diverse geographical locations allowed their correct identification when comparing with the results of rrs gene and ITS analyses. The nodulation of Lupinus gredensis, an endemic species of the west of Spain, by B. canariense supports the European origin of this species.

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