QuEChERS-Based Method for the Determination of Fipronil in Protein Baits and Vespa velutina Larvae by HPLC-DAD and GC-MS

Protein baits containing fipronil as a biocide have shown their effectivity as a control method for Vespa velutina nigrithorax (Lepeletier, 1836) in apiaries. This biocide is not selective for Vespa velutina, so it is important to use the minimum dose to inactivate a nest. Therefore, the aim of this...

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Detalhes bibliográficos
Autores: De la Hera Fernández, Omaira, Izaguirre, Aritza, Rivas Macho, Arrate, Alonso Rojas, Rosa María
Tipo de documento: artigo
Data de publicação:2024
País:España
Recursos:Universidad del País Vasco
Repositório:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/70628
Acesso em linha:http://hdl.handle.net/10810/70628
Access Level:Acceso aberto
Palavra-chave:QuEChERS
chromatography
fipronil
protein bait Vespa velutina larva
metabolism
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spelling QuEChERS-Based Method for the Determination of Fipronil in Protein Baits and Vespa velutina Larvae by HPLC-DAD and GC-MSDe la Hera Fernández, OmairaIzaguirre, AritzaRivas Macho, ArrateAlonso Rojas, Rosa MaríaQuEChERSchromatographyfipronilprotein bait Vespa velutina larvametabolismProtein baits containing fipronil as a biocide have shown their effectivity as a control method for Vespa velutina nigrithorax (Lepeletier, 1836) in apiaries. This biocide is not selective for Vespa velutina, so it is important to use the minimum dose to inactivate a nest. Therefore, the aim of this work was the development of analytical methods for the determination of fipronil in protein baits for quality control purposes and in larvae of Vespa velutina to determine the biocide content after protein bait ingestion and to acquire knowledge on fipronil metabolism in larvae. For this purpose, a Quechers-based HPLC-PDA method was developed and validated for the determination of fipronil in both matrixes. Furthermore, a GC-MS method was developed for the analysis of fipronil and its metabolites in dead Vespa velutina larvae fed with a mash containing 0.01% fipronil. Quechers-based HPLC-DAD allowed for the determination of the fipronil content in baits. Fipronil and the metabolites fipronil sulfone and fipronil sulfide were identified by GC-MS in extracts of larvae fed with a protein mash containing 0.01% fipronil. The transformation of fipronil into fipronil sulfone inside the larvae and the high toxicity of this metabolite open the possibility to produce protein baits with lower biocide concentrations.Omaira de la Hera thanks the University of the Basque Country (UPV/EHU) for her predoctoral grant. Authors thank the Department of Education of Basque Country Government (projects IT1673-22, PUE 2018_1_0007 and PUE 2021_1_008) and the University of the Basque Country (UPV/EHU) (project US21/35) for financial support.MDPI2024202420242024info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/70628reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://www.mdpi.com/2297-8739/11/11/317info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/es/© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).oai:addi.ehu.eus:10810/706282026-06-18T09:23:17Z
dc.title.none.fl_str_mv QuEChERS-Based Method for the Determination of Fipronil in Protein Baits and Vespa velutina Larvae by HPLC-DAD and GC-MS
title QuEChERS-Based Method for the Determination of Fipronil in Protein Baits and Vespa velutina Larvae by HPLC-DAD and GC-MS
spellingShingle QuEChERS-Based Method for the Determination of Fipronil in Protein Baits and Vespa velutina Larvae by HPLC-DAD and GC-MS
De la Hera Fernández, Omaira
QuEChERS
chromatography
fipronil
protein bait Vespa velutina larva
metabolism
title_short QuEChERS-Based Method for the Determination of Fipronil in Protein Baits and Vespa velutina Larvae by HPLC-DAD and GC-MS
title_full QuEChERS-Based Method for the Determination of Fipronil in Protein Baits and Vespa velutina Larvae by HPLC-DAD and GC-MS
title_fullStr QuEChERS-Based Method for the Determination of Fipronil in Protein Baits and Vespa velutina Larvae by HPLC-DAD and GC-MS
title_full_unstemmed QuEChERS-Based Method for the Determination of Fipronil in Protein Baits and Vespa velutina Larvae by HPLC-DAD and GC-MS
title_sort QuEChERS-Based Method for the Determination of Fipronil in Protein Baits and Vespa velutina Larvae by HPLC-DAD and GC-MS
dc.creator.none.fl_str_mv De la Hera Fernández, Omaira
Izaguirre, Aritza
Rivas Macho, Arrate
Alonso Rojas, Rosa María
author De la Hera Fernández, Omaira
author_facet De la Hera Fernández, Omaira
Izaguirre, Aritza
Rivas Macho, Arrate
Alonso Rojas, Rosa María
author_role author
author2 Izaguirre, Aritza
Rivas Macho, Arrate
Alonso Rojas, Rosa María
author2_role author
author
author
dc.subject.none.fl_str_mv QuEChERS
chromatography
fipronil
protein bait Vespa velutina larva
metabolism
topic QuEChERS
chromatography
fipronil
protein bait Vespa velutina larva
metabolism
description Protein baits containing fipronil as a biocide have shown their effectivity as a control method for Vespa velutina nigrithorax (Lepeletier, 1836) in apiaries. This biocide is not selective for Vespa velutina, so it is important to use the minimum dose to inactivate a nest. Therefore, the aim of this work was the development of analytical methods for the determination of fipronil in protein baits for quality control purposes and in larvae of Vespa velutina to determine the biocide content after protein bait ingestion and to acquire knowledge on fipronil metabolism in larvae. For this purpose, a Quechers-based HPLC-PDA method was developed and validated for the determination of fipronil in both matrixes. Furthermore, a GC-MS method was developed for the analysis of fipronil and its metabolites in dead Vespa velutina larvae fed with a mash containing 0.01% fipronil. Quechers-based HPLC-DAD allowed for the determination of the fipronil content in baits. Fipronil and the metabolites fipronil sulfone and fipronil sulfide were identified by GC-MS in extracts of larvae fed with a protein mash containing 0.01% fipronil. The transformation of fipronil into fipronil sulfone inside the larvae and the high toxicity of this metabolite open the possibility to produce protein baits with lower biocide concentrations.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/70628
url http://hdl.handle.net/10810/70628
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.mdpi.com/2297-8739/11/11/317
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/es/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/es/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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