The association of Lactococcus petauri with lactococcosis is older than expected

Lactococcosis is a globally prevalent infectious disease that has a significant economic and sanitary impact on the rainbow trout industry. Lactococcus garvieae has traditionally been considered the only species implicated in the etiology of this disease, but Lactococcus petauri, a new species, has...

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Autores: Vela Alonso, Ana Isabel, Blanco Gutiérrez, María Del Mar, Colussi, Silvia, Charalampos, Kotzamanidis, Prearo, Marino, Altinok, Ilhan, Luigi Acutis, Pier, Volpatti, Donatella, Alba, Patricia, Feltrin, Fabiola, Ianzano, Angela, Domínguez Rodríguez, Lucas José, Fernández-Garayzábal Fernández, José Francisco
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/104409
Acceso en línea:https://hdl.handle.net/20.500.14352/104409
Access Level:acceso abierto
Palabra clave:636.09
Lactococcosis
Lactococcus petauri
Lactococcus garvieae
Identification
Genome sequences
Phenotypic markers
Veterinaria
3109 Ciencias Veterinarias
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repository_id_str
spelling The association of Lactococcus petauri with lactococcosis is older than expectedVela Alonso, Ana IsabelBlanco Gutiérrez, María Del MarColussi, SilviaCharalampos, KotzamanidisPrearo, MarinoAltinok, IlhanLuigi Acutis, PierVolpatti, DonatellaAlba, PatriciaFeltrin, FabiolaIanzano, AngelaDomínguez Rodríguez, Lucas JoséFernández-Garayzábal Fernández, José Francisco636.09LactococcosisLactococcus petauriLactococcus garvieaeIdentificationGenome sequencesPhenotypic markersVeterinaria3109 Ciencias VeterinariasLactococcosis is a globally prevalent infectious disease that has a significant economic and sanitary impact on the rainbow trout industry. Lactococcus garvieae has traditionally been considered the only species implicated in the etiology of this disease, but Lactococcus petauri, a new species, has recently been implicated as another etiological agent. Both species cannot be distinguished by routine methods commonly used in diagnostic laboratories, resulting in their misidentification. In the present study, the identification of 48 isolates initially identified as L. garvieae was studied by determining their in-silico DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values using pairwise comparisons of their whole genome sequences and the genomes of the type strains of L. garvieae and L. petauri. The genome sequences of 37 isolates from countries in which lactococcosis can be considered endemic (Spain, Italy, Türkiye, and Greece) were obtained in this study, and the genomes of 11 isolates were retrieved from the GenBank database. Isolates from Italy, Singapore, Japan, South Korea, India, one Turkish isolate from 2013 and two Spanish isolates recovered in 1992 and 1996 were confirmed as L. garvieae. The remaining isolates from Spain and Türkiye, as well as those from Portugal, Israel, USA, and Greece were identified as L. petauri. Overall, 60.4% of isolates previously identified as L. garvieae were found to be L. petauri. These results confirm the implication of both species in the etiology of lactococcosis and suggest that L. petauri plays a significant role in the epidemiology of this disease. Some of the isolates identified as L. petauri in the present study were isolated three decades ago, indicating that its association with lactococcosis is older than might be expected from the recent descriptions. The commercial Rapid ID32 Strep system was unable to discriminate between L. garvieae and L. petauri. However, both species exhibited some biochemical differences that might serve as phenotypic markers for their presumptive recognition. Consequently, isolates that hydrolyze hippurate and produce acid from sucrose and tagatose could be presumptively recognized as L. petauri, while those that fail these tests could be identified as L. garvieae. The results of this work indicate that great attention should be given to L. petauri in the epidemiology of lactococcosis.ElsevierUniversidad Complutense de Madrid20232023-09-1220232023-09-12journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/104409reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1044092026-06-02T12:44:21Z
dc.title.none.fl_str_mv The association of Lactococcus petauri with lactococcosis is older than expected
title The association of Lactococcus petauri with lactococcosis is older than expected
spellingShingle The association of Lactococcus petauri with lactococcosis is older than expected
Vela Alonso, Ana Isabel
636.09
Lactococcosis
Lactococcus petauri
Lactococcus garvieae
Identification
Genome sequences
Phenotypic markers
Veterinaria
3109 Ciencias Veterinarias
title_short The association of Lactococcus petauri with lactococcosis is older than expected
title_full The association of Lactococcus petauri with lactococcosis is older than expected
title_fullStr The association of Lactococcus petauri with lactococcosis is older than expected
title_full_unstemmed The association of Lactococcus petauri with lactococcosis is older than expected
title_sort The association of Lactococcus petauri with lactococcosis is older than expected
dc.creator.none.fl_str_mv Vela Alonso, Ana Isabel
Blanco Gutiérrez, María Del Mar
Colussi, Silvia
Charalampos, Kotzamanidis
Prearo, Marino
Altinok, Ilhan
Luigi Acutis, Pier
Volpatti, Donatella
Alba, Patricia
Feltrin, Fabiola
Ianzano, Angela
Domínguez Rodríguez, Lucas José
Fernández-Garayzábal Fernández, José Francisco
author Vela Alonso, Ana Isabel
author_facet Vela Alonso, Ana Isabel
Blanco Gutiérrez, María Del Mar
Colussi, Silvia
Charalampos, Kotzamanidis
Prearo, Marino
Altinok, Ilhan
Luigi Acutis, Pier
Volpatti, Donatella
Alba, Patricia
Feltrin, Fabiola
Ianzano, Angela
Domínguez Rodríguez, Lucas José
Fernández-Garayzábal Fernández, José Francisco
author_role author
author2 Blanco Gutiérrez, María Del Mar
Colussi, Silvia
Charalampos, Kotzamanidis
Prearo, Marino
Altinok, Ilhan
Luigi Acutis, Pier
Volpatti, Donatella
Alba, Patricia
Feltrin, Fabiola
Ianzano, Angela
Domínguez Rodríguez, Lucas José
Fernández-Garayzábal Fernández, José Francisco
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 636.09
Lactococcosis
Lactococcus petauri
Lactococcus garvieae
Identification
Genome sequences
Phenotypic markers
Veterinaria
3109 Ciencias Veterinarias
topic 636.09
Lactococcosis
Lactococcus petauri
Lactococcus garvieae
Identification
Genome sequences
Phenotypic markers
Veterinaria
3109 Ciencias Veterinarias
description Lactococcosis is a globally prevalent infectious disease that has a significant economic and sanitary impact on the rainbow trout industry. Lactococcus garvieae has traditionally been considered the only species implicated in the etiology of this disease, but Lactococcus petauri, a new species, has recently been implicated as another etiological agent. Both species cannot be distinguished by routine methods commonly used in diagnostic laboratories, resulting in their misidentification. In the present study, the identification of 48 isolates initially identified as L. garvieae was studied by determining their in-silico DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values using pairwise comparisons of their whole genome sequences and the genomes of the type strains of L. garvieae and L. petauri. The genome sequences of 37 isolates from countries in which lactococcosis can be considered endemic (Spain, Italy, Türkiye, and Greece) were obtained in this study, and the genomes of 11 isolates were retrieved from the GenBank database. Isolates from Italy, Singapore, Japan, South Korea, India, one Turkish isolate from 2013 and two Spanish isolates recovered in 1992 and 1996 were confirmed as L. garvieae. The remaining isolates from Spain and Türkiye, as well as those from Portugal, Israel, USA, and Greece were identified as L. petauri. Overall, 60.4% of isolates previously identified as L. garvieae were found to be L. petauri. These results confirm the implication of both species in the etiology of lactococcosis and suggest that L. petauri plays a significant role in the epidemiology of this disease. Some of the isolates identified as L. petauri in the present study were isolated three decades ago, indicating that its association with lactococcosis is older than might be expected from the recent descriptions. The commercial Rapid ID32 Strep system was unable to discriminate between L. garvieae and L. petauri. However, both species exhibited some biochemical differences that might serve as phenotypic markers for their presumptive recognition. Consequently, isolates that hydrolyze hippurate and produce acid from sucrose and tagatose could be presumptively recognized as L. petauri, while those that fail these tests could be identified as L. garvieae. The results of this work indicate that great attention should be given to L. petauri in the epidemiology of lactococcosis.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-09-12
2023
2023-09-12
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/104409
url https://hdl.handle.net/20.500.14352/104409
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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