Chilean berry Ugni molinae Turcz. fruit and leaves extracts with interesting antioxidant, antimicrobial and tyrosinase inhibitory properties

The knowledge of the biological properties of fruits and leaves of murta (Ugni molinae Turcz.) has been owned by native Chilean culture. The present study investigated the phenolic content, the antioxidant, antimicrobial and anti-tyrosinase activities of different murta fruit and leaves extracts to...

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Autores: López de Dicastillo, Carol, Bustos, Fernanda, Valenzuela, Ximena, López-Carballo, Gracia, Vilariño, Jose Manuel, Galotto, María José
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/376876
Acceso en línea:http://hdl.handle.net/10261/376876
https://api.elsevier.com/content/abstract/scopus_id/85030481911
Access Level:acceso abierto
Palabra clave:Antioxidant
Murta
Natural extract
Antimicrobial
Tyrosinase inhibitor
Polyphenols
antioxidants
antimicrobial properties
polyphenols
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spelling Chilean berry Ugni molinae Turcz. fruit and leaves extracts with interesting antioxidant, antimicrobial and tyrosinase inhibitory propertiesLópez de Dicastillo, CarolBustos, FernandaValenzuela, XimenaLópez-Carballo, GraciaVilariño, Jose ManuelGalotto, María JoséAntioxidantMurtaNatural extractAntimicrobialTyrosinase inhibitorPolyphenolsantioxidantsantimicrobial propertiespolyphenolsThe knowledge of the biological properties of fruits and leaves of murta (Ugni molinae Turcz.) has been owned by native Chilean culture. The present study investigated the phenolic content, the antioxidant, antimicrobial and anti-tyrosinase activities of different murta fruit and leaves extracts to approach their uses on future food, pharmaceutical and cosmetic applications. Extractions of murta fruit and leaves were carried out under water, ethanol and ethanol 50%. Phenolic content of these extracts was measured through Folin Ciocalteu test and the antioxidant power by four different antioxidant systems (ORAC, FRAP, DPPH and TEAC assays) owing to elucidate the main mechanism of antioxidant. Some flavonoids, such as rutin, isoquercitrin and quercitrin hydrate were identified and quantified through HPLC analysis. Antimicrobial activity was determined measuring minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) values against Escherichia coli and Listeria monocytogenes, and the effect of these extracts on L. monocytogenes was confirmed by flow cytometry. Highest contents of polyphenol compounds were obtained in hydroalcoholic extracts (28±1mggallicacid/g dry fruit, and 128±6mggallicacid/g dry leaves). The same trend was found for the values of biological properties: hydroalcoholic extracts showed the strongest activities. Leaves presented higher antioxidant, antimicrobial and anti-tyrosinase properties than murta fruit. Highest antioxidant activity values according to ORAC, FRAP, TEAC and DPPH were 80±8mgTrolox/g, 70±2mgTrolox/g, 87±8mgTrolox/g and 110±12mgTrolox/g, respectively, for murta fruit samples, and 280±10mgTrolox/g, 192±4mgTrolox/g, 286±13mgTrolox/g and 361±13mgTrolox/g, respectively, for murta leaves. These activities were confirmed by HPLC analysis that revealed highest presence of analyzed compounds on leaves hydroalcoholic extract. Regarding to antimicrobial analysis, hydroalcoholic leaves extract presented the highest activity presenting the lowest MIC value for L. monocytogenes (0.07mg/mL). This extract also performed the highest anti-tyrosinase activity (CE50 values of 1.6±0.3 (g/L) and 8.9±1.2 (g/L) for leaves and fruit, respectively).The authors acknowledge the Financial support of CONICYT through the Project “Programa de Financiamiento Basal para Centros Científicos y Tecnológicos de Excelencia” (Project FB0807) and Project Basal USA 1555 – Vridei O81771GL_CONT from the University of Santiago de Chile.Peer reviewedElsevierComisión Nacional de Investigación Científica y Tecnológica (Chile)Universidad de Santiago de ChileLópez de Dicastillo, Carol [0000-0003-0067-9765]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/376876https://api.elsevier.com/content/abstract/scopus_id/85030481911reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésFood research international (Ottawa, Ont.)https://doi.org/10.1016/j.foodres.2017.09.073Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3768762026-05-22T06:33:51Z
dc.title.none.fl_str_mv Chilean berry Ugni molinae Turcz. fruit and leaves extracts with interesting antioxidant, antimicrobial and tyrosinase inhibitory properties
title Chilean berry Ugni molinae Turcz. fruit and leaves extracts with interesting antioxidant, antimicrobial and tyrosinase inhibitory properties
spellingShingle Chilean berry Ugni molinae Turcz. fruit and leaves extracts with interesting antioxidant, antimicrobial and tyrosinase inhibitory properties
López de Dicastillo, Carol
Antioxidant
Murta
Natural extract
Antimicrobial
Tyrosinase inhibitor
Polyphenols
antioxidants
antimicrobial properties
polyphenols
title_short Chilean berry Ugni molinae Turcz. fruit and leaves extracts with interesting antioxidant, antimicrobial and tyrosinase inhibitory properties
title_full Chilean berry Ugni molinae Turcz. fruit and leaves extracts with interesting antioxidant, antimicrobial and tyrosinase inhibitory properties
title_fullStr Chilean berry Ugni molinae Turcz. fruit and leaves extracts with interesting antioxidant, antimicrobial and tyrosinase inhibitory properties
title_full_unstemmed Chilean berry Ugni molinae Turcz. fruit and leaves extracts with interesting antioxidant, antimicrobial and tyrosinase inhibitory properties
title_sort Chilean berry Ugni molinae Turcz. fruit and leaves extracts with interesting antioxidant, antimicrobial and tyrosinase inhibitory properties
dc.creator.none.fl_str_mv López de Dicastillo, Carol
Bustos, Fernanda
Valenzuela, Ximena
López-Carballo, Gracia
Vilariño, Jose Manuel
Galotto, María José
author López de Dicastillo, Carol
author_facet López de Dicastillo, Carol
Bustos, Fernanda
Valenzuela, Ximena
López-Carballo, Gracia
Vilariño, Jose Manuel
Galotto, María José
author_role author
author2 Bustos, Fernanda
Valenzuela, Ximena
López-Carballo, Gracia
Vilariño, Jose Manuel
Galotto, María José
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Comisión Nacional de Investigación Científica y Tecnológica (Chile)
Universidad de Santiago de Chile
López de Dicastillo, Carol [0000-0003-0067-9765]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Antioxidant
Murta
Natural extract
Antimicrobial
Tyrosinase inhibitor
Polyphenols
antioxidants
antimicrobial properties
polyphenols
topic Antioxidant
Murta
Natural extract
Antimicrobial
Tyrosinase inhibitor
Polyphenols
antioxidants
antimicrobial properties
polyphenols
description The knowledge of the biological properties of fruits and leaves of murta (Ugni molinae Turcz.) has been owned by native Chilean culture. The present study investigated the phenolic content, the antioxidant, antimicrobial and anti-tyrosinase activities of different murta fruit and leaves extracts to approach their uses on future food, pharmaceutical and cosmetic applications. Extractions of murta fruit and leaves were carried out under water, ethanol and ethanol 50%. Phenolic content of these extracts was measured through Folin Ciocalteu test and the antioxidant power by four different antioxidant systems (ORAC, FRAP, DPPH and TEAC assays) owing to elucidate the main mechanism of antioxidant. Some flavonoids, such as rutin, isoquercitrin and quercitrin hydrate were identified and quantified through HPLC analysis. Antimicrobial activity was determined measuring minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) values against Escherichia coli and Listeria monocytogenes, and the effect of these extracts on L. monocytogenes was confirmed by flow cytometry. Highest contents of polyphenol compounds were obtained in hydroalcoholic extracts (28±1mggallicacid/g dry fruit, and 128±6mggallicacid/g dry leaves). The same trend was found for the values of biological properties: hydroalcoholic extracts showed the strongest activities. Leaves presented higher antioxidant, antimicrobial and anti-tyrosinase properties than murta fruit. Highest antioxidant activity values according to ORAC, FRAP, TEAC and DPPH were 80±8mgTrolox/g, 70±2mgTrolox/g, 87±8mgTrolox/g and 110±12mgTrolox/g, respectively, for murta fruit samples, and 280±10mgTrolox/g, 192±4mgTrolox/g, 286±13mgTrolox/g and 361±13mgTrolox/g, respectively, for murta leaves. These activities were confirmed by HPLC analysis that revealed highest presence of analyzed compounds on leaves hydroalcoholic extract. Regarding to antimicrobial analysis, hydroalcoholic leaves extract presented the highest activity presenting the lowest MIC value for L. monocytogenes (0.07mg/mL). This extract also performed the highest anti-tyrosinase activity (CE50 values of 1.6±0.3 (g/L) and 8.9±1.2 (g/L) for leaves and fruit, respectively).
publishDate 2017
dc.date.none.fl_str_mv 2017
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/376876
https://api.elsevier.com/content/abstract/scopus_id/85030481911
url http://hdl.handle.net/10261/376876
https://api.elsevier.com/content/abstract/scopus_id/85030481911
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Food research international (Ottawa, Ont.)
https://doi.org/10.1016/j.foodres.2017.09.073
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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