New gene targets for glucagon-like peptide-1 during embryonic development and in undifferentiated pluripotent cells

In humans, glucagon-like peptide (GLP-1) functions during adult life as an incretin hormone with anorexigenic and antidiabetogenic properties. Also, the therapeutic potential of GLP-1 in preventing the adipocyte hyperplasia associated with obesity and in bolstering the maintenance of human mesenchym...

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Detalles Bibliográficos
Autores: Sanz Miguel, María Del Carmen, Blázquez Fernández, Enrique
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/110964
Acceso en línea:https://hdl.handle.net/20.500.14352/110964
Access Level:acceso abierto
Palabra clave:577.1
Biología celular (Biología)
Endocrinología
Bioquímica (Medicina)
2407 Biología Celular
2403 Bioquímica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/110964
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spelling New gene targets for glucagon-like peptide-1 during embryonic development and in undifferentiated pluripotent cellsSanz Miguel, María Del CarmenBlázquez Fernández, Enrique577.1Biología celular (Biología)EndocrinologíaBioquímica (Medicina)2407 Biología Celular2403 BioquímicaIn humans, glucagon-like peptide (GLP-1) functions during adult life as an incretin hormone with anorexigenic and antidiabetogenic properties. Also, the therapeutic potential of GLP-1 in preventing the adipocyte hyperplasia associated with obesity and in bolstering the maintenance of human mesenchymal stem cell (hMSC) stores by promoting the proliferation and cytoprotection of hMSC seems to be relevant. Since these observations suggest a role for GLP-1 during developmental processes, the aim of the present work was to characterize GLP-1 in early development as well as its gene targets in mouse embryonic stem (mES) cells. Mouse embryos E6, E8, and E10.5 and pluripotent mES were used for the inmunodetection of GLP-1 and GLP-1 receptor. Quantitative real-time PCR was used to determine the expression levels of GLP-1R in several tissues from E12.5 mouse embryos. Additionally, GLP-1 gene targets were studied in mES by multiple gene expression analyses. GLP-1 and its receptors were identified in mES and during embryonic development. In pluripotent mES, GLP-1 modified the expression of endodermal, ectodermal, and mesodermal gene markers as well as sonic hedgehog, noggin, members of the fibroblast and hepatic growth factor families, and others involved in pancreatic development. Additionally, GLP-1 promoted the expression of the antiapoptotic gene bcl2 and at the same time reduced proapoptotic caspase genes. Our results indicate that apart from the effects and therapeutic benefits of GLP-1 in adulthood, it may have additional gene targets in mES cells during embryonic life. Furthermore, the pathophysiological implications of GLP-1 imbalance in adulthood may have a counterpart during development.American Physiological SocietyUniversidad Complutense de Madrid20112011-01-0120112011-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/110964reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1109642026-06-02T12:44:21Z
dc.title.none.fl_str_mv New gene targets for glucagon-like peptide-1 during embryonic development and in undifferentiated pluripotent cells
title New gene targets for glucagon-like peptide-1 during embryonic development and in undifferentiated pluripotent cells
spellingShingle New gene targets for glucagon-like peptide-1 during embryonic development and in undifferentiated pluripotent cells
Sanz Miguel, María Del Carmen
577.1
Biología celular (Biología)
Endocrinología
Bioquímica (Medicina)
2407 Biología Celular
2403 Bioquímica
title_short New gene targets for glucagon-like peptide-1 during embryonic development and in undifferentiated pluripotent cells
title_full New gene targets for glucagon-like peptide-1 during embryonic development and in undifferentiated pluripotent cells
title_fullStr New gene targets for glucagon-like peptide-1 during embryonic development and in undifferentiated pluripotent cells
title_full_unstemmed New gene targets for glucagon-like peptide-1 during embryonic development and in undifferentiated pluripotent cells
title_sort New gene targets for glucagon-like peptide-1 during embryonic development and in undifferentiated pluripotent cells
dc.creator.none.fl_str_mv Sanz Miguel, María Del Carmen
Blázquez Fernández, Enrique
author Sanz Miguel, María Del Carmen
author_facet Sanz Miguel, María Del Carmen
Blázquez Fernández, Enrique
author_role author
author2 Blázquez Fernández, Enrique
author2_role author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.1
Biología celular (Biología)
Endocrinología
Bioquímica (Medicina)
2407 Biología Celular
2403 Bioquímica
topic 577.1
Biología celular (Biología)
Endocrinología
Bioquímica (Medicina)
2407 Biología Celular
2403 Bioquímica
description In humans, glucagon-like peptide (GLP-1) functions during adult life as an incretin hormone with anorexigenic and antidiabetogenic properties. Also, the therapeutic potential of GLP-1 in preventing the adipocyte hyperplasia associated with obesity and in bolstering the maintenance of human mesenchymal stem cell (hMSC) stores by promoting the proliferation and cytoprotection of hMSC seems to be relevant. Since these observations suggest a role for GLP-1 during developmental processes, the aim of the present work was to characterize GLP-1 in early development as well as its gene targets in mouse embryonic stem (mES) cells. Mouse embryos E6, E8, and E10.5 and pluripotent mES were used for the inmunodetection of GLP-1 and GLP-1 receptor. Quantitative real-time PCR was used to determine the expression levels of GLP-1R in several tissues from E12.5 mouse embryos. Additionally, GLP-1 gene targets were studied in mES by multiple gene expression analyses. GLP-1 and its receptors were identified in mES and during embryonic development. In pluripotent mES, GLP-1 modified the expression of endodermal, ectodermal, and mesodermal gene markers as well as sonic hedgehog, noggin, members of the fibroblast and hepatic growth factor families, and others involved in pancreatic development. Additionally, GLP-1 promoted the expression of the antiapoptotic gene bcl2 and at the same time reduced proapoptotic caspase genes. Our results indicate that apart from the effects and therapeutic benefits of GLP-1 in adulthood, it may have additional gene targets in mES cells during embryonic life. Furthermore, the pathophysiological implications of GLP-1 imbalance in adulthood may have a counterpart during development.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01
2011
2011-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/110964
url https://hdl.handle.net/20.500.14352/110964
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Physiological Society
publisher.none.fl_str_mv American Physiological Society
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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