Differential expression patterns of Arabinogalactan Proteins in Arabidopsis thaliana reproductive tissues

36 p.-6 fig.

Detalles Bibliográficos
Autores: Pereira, Ana Marta, Masiero, Simona, Nobre, Margarida Sofia, Costa, Mário Luís, Solís, María Teresa, Testillano, Pilar S., Sprunck, Stefanie, Coimbra, Silvia
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/101424
Acceso en línea:http://hdl.handle.net/10261/101424
Access Level:acceso abierto
Palabra clave:Arabinogalactan proteins
Female gametophyte
Funiculus
Pistil
Pollen tube guidance
Transmitting tract
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spelling Differential expression patterns of Arabinogalactan Proteins in Arabidopsis thaliana reproductive tissuesPereira, Ana MartaMasiero, SimonaNobre, Margarida SofiaCosta, Mário LuísSolís, María TeresaTestillano, Pilar S.Sprunck, StefanieCoimbra, SilviaArabinogalactan proteinsFemale gametophyteFuniculusPistilPollen tube guidanceTransmitting tract36 p.-6 fig.Arabinogalactan proteins (AGPs) are heavily glycosylated proteins existing in all members of the plant kingdom and are differentially distributed through distinctive developmental stages. Here, we showed the individual distributions of specific Arabidopsis AGPs: AGP1, AGP9, AGP12, AGP15, and AGP23, throughout reproductive tissues and indicated their possible roles in several reproductive processes. AGP genes specifically expressed in female tissues were identified using available microarray data. This selection was confirmed by promoter analysis using multiple green fluorescent protein fusions to a nuclear localization signal, β-glucuronidase fusions, and in situ hybridization as approaches to confirm the expression patterns of the AGPs. Promoter analysis allowed the detection of a specific and differential presence of these proteins along the pathway followed by the pollen tube during its journey to reach the egg and the central cell inside the embryo sac. AGP1 was expressed in the stigma, style, transmitting tract, and the chalazal and funiculus tissues of the ovules. AGP9 was present along the vasculature of the reproductive tissues and AGP12 was expressed in the stigmatic cells, chalazal and funiculus cells of the ovules, and in the septum. AGP15 was expressed in all pistil tissues, except in the transmitting tract, while AGP23 was specific to the pollen grain and pollen tube. The expression pattern of these AGPs provides new evidence for the detection of a subset of specific AGPs involved in plant reproductive processes, being of significance for this field of study. AGPs are prominent candidates for male–female communication during reproduction.This work was financed by FEDER through the COMPETE programme, and by Portuguese National funds through FCT – Fundação para a Ciência e Tecnologia (Project PTDC/AGR-GPL/115358/2009) and from an FCT PhD grant SFRH/BD/60995/2009 awarded to AMP. This project also benefited from financial support from the COST Action FA0903: ‘Harnessing Plant Reproduction for Crop Improvement’.Peer reviewedOxford University PressConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201420142014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/101424reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1093/jxb/eru300Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1014242026-05-22T06:33:51Z
dc.title.none.fl_str_mv Differential expression patterns of Arabinogalactan Proteins in Arabidopsis thaliana reproductive tissues
title Differential expression patterns of Arabinogalactan Proteins in Arabidopsis thaliana reproductive tissues
spellingShingle Differential expression patterns of Arabinogalactan Proteins in Arabidopsis thaliana reproductive tissues
Pereira, Ana Marta
Arabinogalactan proteins
Female gametophyte
Funiculus
Pistil
Pollen tube guidance
Transmitting tract
title_short Differential expression patterns of Arabinogalactan Proteins in Arabidopsis thaliana reproductive tissues
title_full Differential expression patterns of Arabinogalactan Proteins in Arabidopsis thaliana reproductive tissues
title_fullStr Differential expression patterns of Arabinogalactan Proteins in Arabidopsis thaliana reproductive tissues
title_full_unstemmed Differential expression patterns of Arabinogalactan Proteins in Arabidopsis thaliana reproductive tissues
title_sort Differential expression patterns of Arabinogalactan Proteins in Arabidopsis thaliana reproductive tissues
dc.creator.none.fl_str_mv Pereira, Ana Marta
Masiero, Simona
Nobre, Margarida Sofia
Costa, Mário Luís
Solís, María Teresa
Testillano, Pilar S.
Sprunck, Stefanie
Coimbra, Silvia
author Pereira, Ana Marta
author_facet Pereira, Ana Marta
Masiero, Simona
Nobre, Margarida Sofia
Costa, Mário Luís
Solís, María Teresa
Testillano, Pilar S.
Sprunck, Stefanie
Coimbra, Silvia
author_role author
author2 Masiero, Simona
Nobre, Margarida Sofia
Costa, Mário Luís
Solís, María Teresa
Testillano, Pilar S.
Sprunck, Stefanie
Coimbra, Silvia
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Arabinogalactan proteins
Female gametophyte
Funiculus
Pistil
Pollen tube guidance
Transmitting tract
topic Arabinogalactan proteins
Female gametophyte
Funiculus
Pistil
Pollen tube guidance
Transmitting tract
description 36 p.-6 fig.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/101424
url http://hdl.handle.net/10261/101424
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1093/jxb/eru300
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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