Target-site resistance to ALS-inhibiting herbicides in Stellaria media, Papaver rhoeas, Glebionis segetum and Veronica persica from Ireland
The increasing reliance on acetolactate synthase (ALS)-inhibiting herbicides alone has resulted in the evolution of resistance in key broad-leaf species in cereal farms in Ireland. Our objective was to confirm and characterise the resistance levels and mechanisms in resistance-suspect populations (R...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10459.1/467781 |
| Acceso en línea: | https://doi.org/10.1111/wre.70000 https://hdl.handle.net/10459.1/467781 |
| Access Level: | acceso abierto |
| Palabra clave: | Broad-leaved weeds Common chickweed Common field-speedwell Common poppy |
| Sumario: | The increasing reliance on acetolactate synthase (ALS)-inhibiting herbicides alone has resulted in the evolution of resistance in key broad-leaf species in cereal farms in Ireland. Our objective was to confirm and characterise the resistance levels and mechanisms in resistance-suspect populations (R) of Stellaria media (STEME-R1, STEME-R2 and STEME-R3), Papaver rhoeas (PAPRH-R), Glebionis segetum (GLESE-R) and Veronica persica (VERPE-R) to two different ALS-inhibiting herbicide chemistries, sulfonylurea (SU) and triazolopyrimidine (TP). Following single-dose testing that confirmed inadequate control of all six R populations, dose–response experiments revealed varying resistance levels to SU and/or TP, which were associated with target-site resistance mutations. Weed species populations, STEME-R1 and STEME-R3 (both Trp-574-Leu), and STEME-R2 (combined Trp-574-Leu and Pro-197-Ser; first report for this species), and PAPRH-R (Trp-574-Leu, which was detected for the first time in all population plants, combined with Pro-197-His or Pro-197-Leu) and VERPE-R (Trp-574-Leu; first worldwide case) showed high resistance or cross-resistance to SU and TP. All herbicide-surviving plants tested were heterozygous for single or combined mutations. The G. segetum population, GLESE-R, had multiple substitutions and zygosity (Pro/Leu, Leu, Leu/Gln or Thr/Leu) at Pro-197, which was identified for the first time for this species conferred SU resistance only. Cytochrome P450 inhibitor studies on selected species did not detect metabolism-mediated resistance to SU or TP. Alternative herbicide modes of action alone or in co-formulation with ALS-TP should be used to manage these resistant populations. This study has added new information to the current knowledge of the resistance spectrum to ALS inhibitors in several broad-leaved weeds. |
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