Study of the Vaginal Microbiota in Healthy Women of Reproductive Age

Understanding the characteristics of the vaginal microbiota of our patients allows us to carry out both a personalized therapeutic approach and a closer follow-up in those with microbiota susceptible to dysbiosis. This trial pursues the analysis of the vaginal microbiota of premenopausal women and i...

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Autores: Martinez, MCA, Cazorla, E, Canovas, E, Martinez-Blanch, JF, Chenoll, E, Climent, E, Navarro-Lopez, V
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p10966
Acceso en línea:https://fisabio.portalinvestigacion.com/publicaciones/10966
Access Level:acceso abierto
Palabra clave:vaginal microbiota
dysbiosis
Lactobacillus
Lactobacillus crispatus
microbial communities
16S Ribosomal RNA
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spelling Study of the Vaginal Microbiota in Healthy Women of Reproductive AgeMartinez, MCACazorla, ECanovas, EMartinez-Blanch, JFChenoll, ECliment, ENavarro-Lopez, Vvaginal microbiotadysbiosisLactobacillusLactobacillus crispatusmicrobial communities16S Ribosomal RNAUnderstanding the characteristics of the vaginal microbiota of our patients allows us to carry out both a personalized therapeutic approach and a closer follow-up in those with microbiota susceptible to dysbiosis. This trial pursues the analysis of the vaginal microbiota of premenopausal women and its fluctuations within a four-week follow-up period. Vaginal samples of 76 fertile women were taken at a baseline visit and at a final visit (day 28 +/- 5). To perform a phylogenetic study, we employed massive sequencing techniques to detect the 16S rRNA gene of the vaginal microbiota. The most prevalent vaginal microbial community was type I (34.87%), dominated by Lactobacillus crispatus. Vaginal microbial community types II (Lactobacillus gasseri) and V (Lactobacillus jensenii) were underrepresented in our population. When repeating the sampling process four weeks later, 75% of our patients maintained their initial bacterial community. In the follicular phase, the most recurrent microbiota was type III (Lactobacillus iners); in the periovulatory phase, types III and IV (microbial diversity); finally, in the luteal phase, the most frequent type was IV. The most prevalent vaginal bacterial community in our population was dominated by L. crispatus. The vaginal microbiota was resistant to changes in its bacterial community in 75% of our patients, even between consecutive menstrual cycles.MDPI2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/10966MicroorganismsISSN: 20762607reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p109662026-06-11T12:45:17Z
dc.title.none.fl_str_mv Study of the Vaginal Microbiota in Healthy Women of Reproductive Age
title Study of the Vaginal Microbiota in Healthy Women of Reproductive Age
spellingShingle Study of the Vaginal Microbiota in Healthy Women of Reproductive Age
Martinez, MCA
vaginal microbiota
dysbiosis
Lactobacillus
Lactobacillus crispatus
microbial communities
16S Ribosomal RNA
title_short Study of the Vaginal Microbiota in Healthy Women of Reproductive Age
title_full Study of the Vaginal Microbiota in Healthy Women of Reproductive Age
title_fullStr Study of the Vaginal Microbiota in Healthy Women of Reproductive Age
title_full_unstemmed Study of the Vaginal Microbiota in Healthy Women of Reproductive Age
title_sort Study of the Vaginal Microbiota in Healthy Women of Reproductive Age
dc.creator.none.fl_str_mv Martinez, MCA
Cazorla, E
Canovas, E
Martinez-Blanch, JF
Chenoll, E
Climent, E
Navarro-Lopez, V
author Martinez, MCA
author_facet Martinez, MCA
Cazorla, E
Canovas, E
Martinez-Blanch, JF
Chenoll, E
Climent, E
Navarro-Lopez, V
author_role author
author2 Cazorla, E
Canovas, E
Martinez-Blanch, JF
Chenoll, E
Climent, E
Navarro-Lopez, V
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv vaginal microbiota
dysbiosis
Lactobacillus
Lactobacillus crispatus
microbial communities
16S Ribosomal RNA
topic vaginal microbiota
dysbiosis
Lactobacillus
Lactobacillus crispatus
microbial communities
16S Ribosomal RNA
description Understanding the characteristics of the vaginal microbiota of our patients allows us to carry out both a personalized therapeutic approach and a closer follow-up in those with microbiota susceptible to dysbiosis. This trial pursues the analysis of the vaginal microbiota of premenopausal women and its fluctuations within a four-week follow-up period. Vaginal samples of 76 fertile women were taken at a baseline visit and at a final visit (day 28 +/- 5). To perform a phylogenetic study, we employed massive sequencing techniques to detect the 16S rRNA gene of the vaginal microbiota. The most prevalent vaginal microbial community was type I (34.87%), dominated by Lactobacillus crispatus. Vaginal microbial community types II (Lactobacillus gasseri) and V (Lactobacillus jensenii) were underrepresented in our population. When repeating the sampling process four weeks later, 75% of our patients maintained their initial bacterial community. In the follicular phase, the most recurrent microbiota was type III (Lactobacillus iners); in the periovulatory phase, types III and IV (microbial diversity); finally, in the luteal phase, the most frequent type was IV. The most prevalent vaginal bacterial community in our population was dominated by L. crispatus. The vaginal microbiota was resistant to changes in its bacterial community in 75% of our patients, even between consecutive menstrual cycles.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/10966
url https://fisabio.portalinvestigacion.com/publicaciones/10966
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Microorganisms
ISSN: 20762607
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
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