A Comparative Analysis of DNA Metabarcoding and Morphological Identification in Diatoms Reveals Similar Patterns of Environmental Response

Diatoms are widely used as environmental sentinels and are commonly studied through morphological identification. However, this method requires specialist knowledge, which is becoming increasingly scarce, resulting in species identification becoming costly, time-consuming, and difficult. DNA metabar...

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Autores: Gonzalez-Saldias, Fernanda, Gomà Martínez, Joan, Garcés Pastor, Sandra, Wangensteen Fuentes, Owen S. (Simon), Pèlachs, Albert, Pérez Haase, Aaron
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/227853
Acceso en línea:https://hdl.handle.net/2445/227853
Access Level:acceso abierto
Palabra clave:Diatomees
ADN antic
Diatoms
Fossil DNA
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spelling A Comparative Analysis of DNA Metabarcoding and Morphological Identification in Diatoms Reveals Similar Patterns of Environmental ResponseGonzalez-Saldias, FernandaGomà Martínez, JoanGarcés Pastor, SandraWangensteen Fuentes, Owen S. (Simon)Pèlachs, AlbertPérez Haase, AaronDiatomeesADN anticDiatomsFossil DNADiatoms are widely used as environmental sentinels and are commonly studied through morphological identification. However, this method requires specialist knowledge, which is becoming increasingly scarce, resulting in species identification becoming costly, time-consuming, and difficult. DNA metabarcoding offers an alternative method to semiautomate some identification processes to circumvent these difficulties, thus minimizing identification costs while providing objective and reliable information. However, discrepancies between marker genes and morphological identification remain unsolved. This study investigated the 66 diatom communities on 26 high mountain mires in the Pyrenees by identifying morphological species and sequencing the Cytochrome c oxidase I (COI) and 18S rRNA markers. We assessed whether morphological and molecular approaches reveal the same patterns of alpha and beta diversity. Our results showed a strong correlation between beta diversity patterns of diatoms obtained by morphological identification and genetic markers. In contrast, alpha diversity calculated through molecular studies underestimated species richness and the Shannon index. The diatom community was chiefly driven by pH, Mg, and temperature, reflecting these patterns in morphological and molecular data. DNA metabarcoding also proved to represent diatom community patterns and the roles of the environmental drivers as clearly as in morphological identifications in high mountain mire diatom communities.John Wiley & Sons2026202620252026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12 p.application/pdfhttps://hdl.handle.net/2445/227853Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1002/ece3.72644Ecology and Evolution, 2025, vol. 16, num.2, p. 1-12https://doi.org/10.1002/ece3.72644cc-by (c) Fernanda Gonzalez-Saldias et al., 2025http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2278532026-05-29T05:05:01Z
dc.title.none.fl_str_mv A Comparative Analysis of DNA Metabarcoding and Morphological Identification in Diatoms Reveals Similar Patterns of Environmental Response
title A Comparative Analysis of DNA Metabarcoding and Morphological Identification in Diatoms Reveals Similar Patterns of Environmental Response
spellingShingle A Comparative Analysis of DNA Metabarcoding and Morphological Identification in Diatoms Reveals Similar Patterns of Environmental Response
Gonzalez-Saldias, Fernanda
Diatomees
ADN antic
Diatoms
Fossil DNA
title_short A Comparative Analysis of DNA Metabarcoding and Morphological Identification in Diatoms Reveals Similar Patterns of Environmental Response
title_full A Comparative Analysis of DNA Metabarcoding and Morphological Identification in Diatoms Reveals Similar Patterns of Environmental Response
title_fullStr A Comparative Analysis of DNA Metabarcoding and Morphological Identification in Diatoms Reveals Similar Patterns of Environmental Response
title_full_unstemmed A Comparative Analysis of DNA Metabarcoding and Morphological Identification in Diatoms Reveals Similar Patterns of Environmental Response
title_sort A Comparative Analysis of DNA Metabarcoding and Morphological Identification in Diatoms Reveals Similar Patterns of Environmental Response
dc.creator.none.fl_str_mv Gonzalez-Saldias, Fernanda
Gomà Martínez, Joan
Garcés Pastor, Sandra
Wangensteen Fuentes, Owen S. (Simon)
Pèlachs, Albert
Pérez Haase, Aaron
author Gonzalez-Saldias, Fernanda
author_facet Gonzalez-Saldias, Fernanda
Gomà Martínez, Joan
Garcés Pastor, Sandra
Wangensteen Fuentes, Owen S. (Simon)
Pèlachs, Albert
Pérez Haase, Aaron
author_role author
author2 Gomà Martínez, Joan
Garcés Pastor, Sandra
Wangensteen Fuentes, Owen S. (Simon)
Pèlachs, Albert
Pérez Haase, Aaron
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Diatomees
ADN antic
Diatoms
Fossil DNA
topic Diatomees
ADN antic
Diatoms
Fossil DNA
description Diatoms are widely used as environmental sentinels and are commonly studied through morphological identification. However, this method requires specialist knowledge, which is becoming increasingly scarce, resulting in species identification becoming costly, time-consuming, and difficult. DNA metabarcoding offers an alternative method to semiautomate some identification processes to circumvent these difficulties, thus minimizing identification costs while providing objective and reliable information. However, discrepancies between marker genes and morphological identification remain unsolved. This study investigated the 66 diatom communities on 26 high mountain mires in the Pyrenees by identifying morphological species and sequencing the Cytochrome c oxidase I (COI) and 18S rRNA markers. We assessed whether morphological and molecular approaches reveal the same patterns of alpha and beta diversity. Our results showed a strong correlation between beta diversity patterns of diatoms obtained by morphological identification and genetic markers. In contrast, alpha diversity calculated through molecular studies underestimated species richness and the Shannon index. The diatom community was chiefly driven by pH, Mg, and temperature, reflecting these patterns in morphological and molecular data. DNA metabarcoding also proved to represent diatom community patterns and the roles of the environmental drivers as clearly as in morphological identifications in high mountain mire diatom communities.
publishDate 2025
dc.date.none.fl_str_mv 2025
2026
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/227853
url https://hdl.handle.net/2445/227853
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1002/ece3.72644
Ecology and Evolution, 2025, vol. 16, num.2, p. 1-12
https://doi.org/10.1002/ece3.72644
dc.rights.none.fl_str_mv cc-by (c) Fernanda Gonzalez-Saldias et al., 2025
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Fernanda Gonzalez-Saldias et al., 2025
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12 p.
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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