Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell Llnes compared to primary osteoblasts

The miR-320a regulates a number of genes involved in various physiological processes. In particular, it has been reported as a tumor suppressor in several types of human cancers and involved in osteoporotic fracture and osteoblast function. Hence, the role of miR-320a has been evaluated in tumor cel...

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Autores: Ugarte Corbalán, Laura de, 1988-, Balcells, Susana, Güerri Fernández, Roberto, Grinberg, Daniel, Díez Pérez, Adolfo, Nogués Solán, Xavier, Garcia Giralt, Natàlia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/46579
Acceso en línea:http://hdl.handle.net/10230/46579
http://dx.doi.org/10.3390/app10082852
Access Level:acceso abierto
Palabra clave:Osteosarcoma
MG-63
U2OS
Primary osteoblasts
miR-320a
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spelling Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell Llnes compared to primary osteoblastsUgarte Corbalán, Laura de, 1988-Balcells, SusanaGüerri Fernández, RobertoGrinberg, DanielDíez Pérez, AdolfoNogués Solán, XavierGarcia Giralt, NatàliaOsteosarcomaMG-63U2OSPrimary osteoblastsmiR-320aThe miR-320a regulates a number of genes involved in various physiological processes. In particular, it has been reported as a tumor suppressor in several types of human cancers and involved in osteoporotic fracture and osteoblast function. Hence, the role of miR-320a has been evaluated in tumor cells and in primary cells in a separated context, but its effect has never been explored in a comparative manner. The present study aims to evaluate the cellular effects of miR-320a on human osteosarcoma cell lines (MG-63 and U2OS) compared to that on primary human osteoblasts (hOBs). miR-320a was either overexpressed or inhibited in all cell lines, and cell proliferation and viability were analyzed. Additionally, the effects of miR-320a on matrix mineralization, alkaline phosphatase activity, and oxidative stress were also evaluated in order to assess osteoblast functionality. In osteosarcoma cells, miR-320a overexpression reduced cell viability and proliferation, while in hOB cell viability was not affected and proliferation even was increased. The overexpression of miR-320a in both osteosarcoma cells and hOBs reduced the mineralization capacity. Finally, an increased oxidative stress was detected in all cells after miR-320a overexpression mainly in osteosarcoma. In conclusion, the overexpression of miR-320a increased stress oxidation levels, which could be involved in the reduced osteoblast performance, even though the cell viability was only affected in osteosarcoma cells.This research was supported by the CIBER on Frailty and Healthy Ageing (CIBERFES; grant number: CB16/10/00245), the CIBERER (grant number: U720), FEDER funds, and grants from the Science and Innovation Ministry (ISCIII; grant numbers: PI16/01860 and PI13/00116; SAF2016-75948-R). L.D.-U. was granted with a PFIS predoctoral fellowship from the ISCIII.MDPI202120212020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/46579http://dx.doi.org/10.3390/app10082852reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/grantAgreement/ES/1PE/SAF2016-75948-R© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/465792026-05-29T05:05:01Z
dc.title.none.fl_str_mv Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell Llnes compared to primary osteoblasts
title Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell Llnes compared to primary osteoblasts
spellingShingle Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell Llnes compared to primary osteoblasts
Ugarte Corbalán, Laura de, 1988-
Osteosarcoma
MG-63
U2OS
Primary osteoblasts
miR-320a
title_short Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell Llnes compared to primary osteoblasts
title_full Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell Llnes compared to primary osteoblasts
title_fullStr Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell Llnes compared to primary osteoblasts
title_full_unstemmed Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell Llnes compared to primary osteoblasts
title_sort Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell Llnes compared to primary osteoblasts
dc.creator.none.fl_str_mv Ugarte Corbalán, Laura de, 1988-
Balcells, Susana
Güerri Fernández, Roberto
Grinberg, Daniel
Díez Pérez, Adolfo
Nogués Solán, Xavier
Garcia Giralt, Natàlia
author Ugarte Corbalán, Laura de, 1988-
author_facet Ugarte Corbalán, Laura de, 1988-
Balcells, Susana
Güerri Fernández, Roberto
Grinberg, Daniel
Díez Pérez, Adolfo
Nogués Solán, Xavier
Garcia Giralt, Natàlia
author_role author
author2 Balcells, Susana
Güerri Fernández, Roberto
Grinberg, Daniel
Díez Pérez, Adolfo
Nogués Solán, Xavier
Garcia Giralt, Natàlia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Osteosarcoma
MG-63
U2OS
Primary osteoblasts
miR-320a
topic Osteosarcoma
MG-63
U2OS
Primary osteoblasts
miR-320a
description The miR-320a regulates a number of genes involved in various physiological processes. In particular, it has been reported as a tumor suppressor in several types of human cancers and involved in osteoporotic fracture and osteoblast function. Hence, the role of miR-320a has been evaluated in tumor cells and in primary cells in a separated context, but its effect has never been explored in a comparative manner. The present study aims to evaluate the cellular effects of miR-320a on human osteosarcoma cell lines (MG-63 and U2OS) compared to that on primary human osteoblasts (hOBs). miR-320a was either overexpressed or inhibited in all cell lines, and cell proliferation and viability were analyzed. Additionally, the effects of miR-320a on matrix mineralization, alkaline phosphatase activity, and oxidative stress were also evaluated in order to assess osteoblast functionality. In osteosarcoma cells, miR-320a overexpression reduced cell viability and proliferation, while in hOB cell viability was not affected and proliferation even was increased. The overexpression of miR-320a in both osteosarcoma cells and hOBs reduced the mineralization capacity. Finally, an increased oxidative stress was detected in all cells after miR-320a overexpression mainly in osteosarcoma. In conclusion, the overexpression of miR-320a increased stress oxidation levels, which could be involved in the reduced osteoblast performance, even though the cell viability was only affected in osteosarcoma cells.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/46579
http://dx.doi.org/10.3390/app10082852
url http://hdl.handle.net/10230/46579
http://dx.doi.org/10.3390/app10082852
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/ES/1PE/SAF2016-75948-R
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
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