Sperm functional and morphometric differences between Iberian and European ecotypes of capercaillie (Tetrao urogallus L.)

The capercaillie (Tetrao urogallus L.) is a threatened forest bird with distinct Iberian and European ecotypes. This study examines the functional and morphometric differences between the sperm of these ecotypes. In a first experiment, semen volume, sperm concentration, motility, viability, and DNA...

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Autores: Gomez-Delgado, J., Martínez-Madrid, B., Toledano-Díaz, Adolfo, Castaño, C., Gómez-Crespo, A., Aguilar, G. de Pedro, Cubero, D., Kowalczyk, A., Łukaszewicz, E., Santiago-Moreno, Julián
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/409775
Acceso en línea:http://hdl.handle.net/10261/409775
Access Level:acceso abierto
Palabra clave:Capercaillie
Sperm morphometry
DNA integrity
Reproductive biology
Iberian capercaillie
European capercaillie
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spelling Sperm functional and morphometric differences between Iberian and European ecotypes of capercaillie (Tetrao urogallus L.)Gomez-Delgado, J.Martínez-Madrid, B.Toledano-Díaz, AdolfoCastaño, C.Gómez-Crespo, A.Aguilar, G. de PedroCubero, D.Kowalczyk, A.Łukaszewicz, E.Santiago-Moreno, JuliánCapercaillieSperm morphometryDNA integrityReproductive biologyIberian capercaillieEuropean capercaillieThe capercaillie (Tetrao urogallus L.) is a threatened forest bird with distinct Iberian and European ecotypes. This study examines the functional and morphometric differences between the sperm of these ecotypes. In a first experiment, semen volume, sperm concentration, motility, viability, and DNA integrity were assessed in 'Iberian' and 'European' capercaillies, both maintained at breeding centres in Spain. In a second experiment, morphometric traits were measured in Iberian capercaillies maintained at a breeding centre in Spain, and in European capercaillies also maintained at breeding centres in Spain (EmS birds) and in Poland (EmP birds). In the first experiment, the European males produced larger ejaculate volumes (39.80 ± 3.56 µL) than the Iberian males (29.68 ± 4.64 µL). However, the Iberian males returned significantly higher sperm concentrations (501.99 ± 83.90 × 10⁶ spz/mL vs. 77.66 ± 26.09 × 10⁶ spz/mL). In the second experiment, the origin of the birds also affected (P < 0.001) sperm head dimensions. These were smaller in the EmP birds compared to the EmS birds (P < 0.01), and compared to Iberian males (always maintained in Spain) (P < 0.001). Within each of these groups, three sperm subpopulations were identified according to head dimensions, with differences (P < 0.001) between these groups in terms of the proportion of each subpopulation. This is the first comprehensive study of sperm morphometric characteristics in these capercaillie ecotypes. These results may provide critical insights into the reproductive and evolutionary strategies of capercaillies and contribute to improving the success of reproductive technologies across different ecotypes and populations.This work was financed via grant TED2021–130581B-I00 funded by the MCIN/AEI/ 10.13039/501100011033 and the European Union NextGenerationEU/PRTR.Peer reviewedElsevierAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252026info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/409775reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//TED2021–130581B-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.anireprosci.2025.108071https://doi.org/10.1016/j.anireprosci.2025.108071Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4097752026-05-22T06:33:51Z
dc.title.none.fl_str_mv Sperm functional and morphometric differences between Iberian and European ecotypes of capercaillie (Tetrao urogallus L.)
title Sperm functional and morphometric differences between Iberian and European ecotypes of capercaillie (Tetrao urogallus L.)
spellingShingle Sperm functional and morphometric differences between Iberian and European ecotypes of capercaillie (Tetrao urogallus L.)
Gomez-Delgado, J.
Capercaillie
Sperm morphometry
DNA integrity
Reproductive biology
Iberian capercaillie
European capercaillie
title_short Sperm functional and morphometric differences between Iberian and European ecotypes of capercaillie (Tetrao urogallus L.)
title_full Sperm functional and morphometric differences between Iberian and European ecotypes of capercaillie (Tetrao urogallus L.)
title_fullStr Sperm functional and morphometric differences between Iberian and European ecotypes of capercaillie (Tetrao urogallus L.)
title_full_unstemmed Sperm functional and morphometric differences between Iberian and European ecotypes of capercaillie (Tetrao urogallus L.)
title_sort Sperm functional and morphometric differences between Iberian and European ecotypes of capercaillie (Tetrao urogallus L.)
dc.creator.none.fl_str_mv Gomez-Delgado, J.
Martínez-Madrid, B.
Toledano-Díaz, Adolfo
Castaño, C.
Gómez-Crespo, A.
Aguilar, G. de Pedro
Cubero, D.
Kowalczyk, A.
Łukaszewicz, E.
Santiago-Moreno, Julián
author Gomez-Delgado, J.
author_facet Gomez-Delgado, J.
Martínez-Madrid, B.
Toledano-Díaz, Adolfo
Castaño, C.
Gómez-Crespo, A.
Aguilar, G. de Pedro
Cubero, D.
Kowalczyk, A.
Łukaszewicz, E.
Santiago-Moreno, Julián
author_role author
author2 Martínez-Madrid, B.
Toledano-Díaz, Adolfo
Castaño, C.
Gómez-Crespo, A.
Aguilar, G. de Pedro
Cubero, D.
Kowalczyk, A.
Łukaszewicz, E.
Santiago-Moreno, Julián
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Capercaillie
Sperm morphometry
DNA integrity
Reproductive biology
Iberian capercaillie
European capercaillie
topic Capercaillie
Sperm morphometry
DNA integrity
Reproductive biology
Iberian capercaillie
European capercaillie
description The capercaillie (Tetrao urogallus L.) is a threatened forest bird with distinct Iberian and European ecotypes. This study examines the functional and morphometric differences between the sperm of these ecotypes. In a first experiment, semen volume, sperm concentration, motility, viability, and DNA integrity were assessed in 'Iberian' and 'European' capercaillies, both maintained at breeding centres in Spain. In a second experiment, morphometric traits were measured in Iberian capercaillies maintained at a breeding centre in Spain, and in European capercaillies also maintained at breeding centres in Spain (EmS birds) and in Poland (EmP birds). In the first experiment, the European males produced larger ejaculate volumes (39.80 ± 3.56 µL) than the Iberian males (29.68 ± 4.64 µL). However, the Iberian males returned significantly higher sperm concentrations (501.99 ± 83.90 × 10⁶ spz/mL vs. 77.66 ± 26.09 × 10⁶ spz/mL). In the second experiment, the origin of the birds also affected (P < 0.001) sperm head dimensions. These were smaller in the EmP birds compared to the EmS birds (P < 0.01), and compared to Iberian males (always maintained in Spain) (P < 0.001). Within each of these groups, three sperm subpopulations were identified according to head dimensions, with differences (P < 0.001) between these groups in terms of the proportion of each subpopulation. This is the first comprehensive study of sperm morphometric characteristics in these capercaillie ecotypes. These results may provide critical insights into the reproductive and evolutionary strategies of capercaillies and contribute to improving the success of reproductive technologies across different ecotypes and populations.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/409775
url http://hdl.handle.net/10261/409775
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI//TED2021–130581B-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.anireprosci.2025.108071
https://doi.org/10.1016/j.anireprosci.2025.108071
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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