Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring

Over the last years, optical biosensors based on plasmonic nanomaterials have gained great scientific interest due to their unquestionable advantages compared to other biosensing technologies. They can achieve sensitive, direct, and label-free analysis with exceptional potential for multiplexing and...

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Autores: Camaló Vila, Judith, Castro Aguirre, Nerea, López Muñoz, Gerardo A., Ferret-Miñana, Ainhoa, De Chiara, Francesco, Ramón Azcón, Javier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/182197
Acceso en línea:https://hdl.handle.net/2445/182197
Access Level:acceso abierto
Palabra clave:Biosensors
Cultiu cel·lular
Cell culture
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spelling Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion MonitoringCamaló Vila, JudithCastro Aguirre, NereaLópez Muñoz, Gerardo A.Ferret-Miñana, AinhoaDe Chiara, FrancescoRamón Azcón, JavierBiosensorsCultiu cel·lularBiosensorsCell cultureOver the last years, optical biosensors based on plasmonic nanomaterials have gained great scientific interest due to their unquestionable advantages compared to other biosensing technologies. They can achieve sensitive, direct, and label-free analysis with exceptional potential for multiplexing and miniaturization. Recently, it has been demonstrated the potential of using optical discs as high throughput nanotemplates for the development of plasmonic biosensors in a cost-effective way. This work is a pilot study focused on the development of an integrated plasmonic biosensor for the monitoring of cell adhesion and growth of human retinal pigmented cell line (ARPE-19) under different media conditions (0 and 2% of FBS). We observed an increase of the plasmonic band displacement under 2% FBS compared to 0% conditions over time (1, 3, and 5 h). These preliminary results show that the proposed plasmonic biosensing approach is a direct, non-destructive, and real-time tool that could be employed in the study of living cells behavior and culture conditions. Furthermore, this setup could assess the viability of the cells and their growth over time with low variability between the technical replicates improving the experimental replicability.Frontiers2022202220212022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion9 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/182197Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3389/fbioe.2021.799325Frontiers In Bioengineering And Biotechnology, 2021, vol. 9https://doi.org/10.3389/fbioe.2021.799325info:eu-repo/grantAgreement/EC/H2020/714317info:eu-repo/grantAgreement/EC/H2020/863037cc by (c) Camaló Vila, Judith et al, 2021http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1821972026-05-29T05:05:01Z
dc.title.none.fl_str_mv Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring
title Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring
spellingShingle Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring
Camaló Vila, Judith
Biosensors
Cultiu cel·lular
Biosensors
Cell culture
title_short Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring
title_full Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring
title_fullStr Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring
title_full_unstemmed Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring
title_sort Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring
dc.creator.none.fl_str_mv Camaló Vila, Judith
Castro Aguirre, Nerea
López Muñoz, Gerardo A.
Ferret-Miñana, Ainhoa
De Chiara, Francesco
Ramón Azcón, Javier
author Camaló Vila, Judith
author_facet Camaló Vila, Judith
Castro Aguirre, Nerea
López Muñoz, Gerardo A.
Ferret-Miñana, Ainhoa
De Chiara, Francesco
Ramón Azcón, Javier
author_role author
author2 Castro Aguirre, Nerea
López Muñoz, Gerardo A.
Ferret-Miñana, Ainhoa
De Chiara, Francesco
Ramón Azcón, Javier
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biosensors
Cultiu cel·lular
Biosensors
Cell culture
topic Biosensors
Cultiu cel·lular
Biosensors
Cell culture
description Over the last years, optical biosensors based on plasmonic nanomaterials have gained great scientific interest due to their unquestionable advantages compared to other biosensing technologies. They can achieve sensitive, direct, and label-free analysis with exceptional potential for multiplexing and miniaturization. Recently, it has been demonstrated the potential of using optical discs as high throughput nanotemplates for the development of plasmonic biosensors in a cost-effective way. This work is a pilot study focused on the development of an integrated plasmonic biosensor for the monitoring of cell adhesion and growth of human retinal pigmented cell line (ARPE-19) under different media conditions (0 and 2% of FBS). We observed an increase of the plasmonic band displacement under 2% FBS compared to 0% conditions over time (1, 3, and 5 h). These preliminary results show that the proposed plasmonic biosensing approach is a direct, non-destructive, and real-time tool that could be employed in the study of living cells behavior and culture conditions. Furthermore, this setup could assess the viability of the cells and their growth over time with low variability between the technical replicates improving the experimental replicability.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/182197
url https://hdl.handle.net/2445/182197
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fbioe.2021.799325
Frontiers In Bioengineering And Biotechnology, 2021, vol. 9
https://doi.org/10.3389/fbioe.2021.799325
info:eu-repo/grantAgreement/EC/H2020/714317
info:eu-repo/grantAgreement/EC/H2020/863037
dc.rights.none.fl_str_mv cc by (c) Camaló Vila, Judith et al, 2021
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Camaló Vila, Judith et al, 2021
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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