Cell Stress by Phosphate of Two Protozoa Tetrahymena thermophila and Tetrahymena pyriformis

Phosphorus is one of the bioelements most needed as a compound cell by living organisms. Phosphorus is involved in several pathologies: in human with bone and kidney diseases, in mammals with metabolism disorder (glucose, insulin¿¿¿), in microorganisms whose phosphorus is involved in cell growth. Ph...

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Autores: Papa Daouda, Mar, El Khalfi, B., Pérez-Castiñeira, J. R., Serrano, Aurelio, Soikri, A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/178174
Acceso en línea:http://hdl.handle.net/10261/178174
Access Level:acceso abierto
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spelling Cell Stress by Phosphate of Two Protozoa Tetrahymena thermophila and Tetrahymena pyriformisPapa Daouda, MarEl Khalfi, B.Pérez-Castiñeira, J. R.Serrano, AurelioSoikri, A.Phosphorus is one of the bioelements most needed as a compound cell by living organisms. Phosphorus is involved in several pathologies: in human with bone and kidney diseases, in mammals with metabolism disorder (glucose, insulin¿¿¿), in microorganisms whose phosphorus is involved in cell growth. Phosphorus has various forms including pyrophosphate, a by-product of multiple pathways of biosynthesis. Enzymes that hydrolyze pyrophosphate are called inorganic pyrophosphatases (PPases). Two major types of inorganic pyrophosphatases are distinguished: the soluble pyrophosphatases (sPPases) and the membrane pyrophosphatases (mPPases or H+/Na+-PPases). They play a key role in the control of intracellular inorganic pyrophosphate level and produce an important ions gradient (H+ or Na+) to the cells. In this work, we primarily focused on the physiological study in a phosphate-poor medium of two models Tetrahymena thermophila and Tetrahymena pyriformis , following the mobility, the growth and the morphology of cells. Secondly, we evaluated the enzymatic activity of soluble and membrane pyrophosphatases in both species grown in the same complex medium. A decrease of cell growth is correlated with unusual morphologies and different mobility in the stress medium. The measurement of soluble and membrane inorganic pyrophosphatases activities also shows a decrease which illustrates the lack of phosphate found in the stress medium. Deficiency of phosphate is a limiting factor for protozoan growth. These results indicate that Tetrahymena can be used as a model of cellular stress and consists of a target to study inorganic pyrophosphatases for a better understanding of phosphate cycle in higher organisms.Peer ReviewedScientific Research PublishingConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920172019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/178174reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1781742026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cell Stress by Phosphate of Two Protozoa Tetrahymena thermophila and Tetrahymena pyriformis
title Cell Stress by Phosphate of Two Protozoa Tetrahymena thermophila and Tetrahymena pyriformis
spellingShingle Cell Stress by Phosphate of Two Protozoa Tetrahymena thermophila and Tetrahymena pyriformis
Papa Daouda, Mar
title_short Cell Stress by Phosphate of Two Protozoa Tetrahymena thermophila and Tetrahymena pyriformis
title_full Cell Stress by Phosphate of Two Protozoa Tetrahymena thermophila and Tetrahymena pyriformis
title_fullStr Cell Stress by Phosphate of Two Protozoa Tetrahymena thermophila and Tetrahymena pyriformis
title_full_unstemmed Cell Stress by Phosphate of Two Protozoa Tetrahymena thermophila and Tetrahymena pyriformis
title_sort Cell Stress by Phosphate of Two Protozoa Tetrahymena thermophila and Tetrahymena pyriformis
dc.creator.none.fl_str_mv Papa Daouda, Mar
El Khalfi, B.
Pérez-Castiñeira, J. R.
Serrano, Aurelio
Soikri, A.
author Papa Daouda, Mar
author_facet Papa Daouda, Mar
El Khalfi, B.
Pérez-Castiñeira, J. R.
Serrano, Aurelio
Soikri, A.
author_role author
author2 El Khalfi, B.
Pérez-Castiñeira, J. R.
Serrano, Aurelio
Soikri, A.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Phosphorus is one of the bioelements most needed as a compound cell by living organisms. Phosphorus is involved in several pathologies: in human with bone and kidney diseases, in mammals with metabolism disorder (glucose, insulin¿¿¿), in microorganisms whose phosphorus is involved in cell growth. Phosphorus has various forms including pyrophosphate, a by-product of multiple pathways of biosynthesis. Enzymes that hydrolyze pyrophosphate are called inorganic pyrophosphatases (PPases). Two major types of inorganic pyrophosphatases are distinguished: the soluble pyrophosphatases (sPPases) and the membrane pyrophosphatases (mPPases or H+/Na+-PPases). They play a key role in the control of intracellular inorganic pyrophosphate level and produce an important ions gradient (H+ or Na+) to the cells. In this work, we primarily focused on the physiological study in a phosphate-poor medium of two models Tetrahymena thermophila and Tetrahymena pyriformis , following the mobility, the growth and the morphology of cells. Secondly, we evaluated the enzymatic activity of soluble and membrane pyrophosphatases in both species grown in the same complex medium. A decrease of cell growth is correlated with unusual morphologies and different mobility in the stress medium. The measurement of soluble and membrane inorganic pyrophosphatases activities also shows a decrease which illustrates the lack of phosphate found in the stress medium. Deficiency of phosphate is a limiting factor for protozoan growth. These results indicate that Tetrahymena can be used as a model of cellular stress and consists of a target to study inorganic pyrophosphatases for a better understanding of phosphate cycle in higher organisms.
publishDate 2017
dc.date.none.fl_str_mv 2017
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/178174
url http://hdl.handle.net/10261/178174
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Scientific Research Publishing
publisher.none.fl_str_mv Scientific Research Publishing
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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