A Validated Methodology for Genetic Identification of Tuna Species (Genus Thunnus)

Tuna species of the genus Thunnus, such as the bluefin tunas, are some of the most important and yet most endangered trade fish in the world. Identification of these species in traded forms, however, may be difficult depending on the presentation of the products, which may hamper conservation effort...

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Detalles Bibliográficos
Autores: Viñas de Puig, Jordi, Tudela, Sergi
Tipo de recurso: artículo
Fecha de publicación:2009
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/3594
Acceso en línea:http://hdl.handle.net/10256/3594
Access Level:acceso abierto
Palabra clave:Tonyina -- Genètica
Tuna -- Genetics
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spelling A Validated Methodology for Genetic Identification of Tuna Species (Genus Thunnus)Viñas de Puig, JordiTudela, SergiTonyina -- GenèticaTuna -- GeneticsTuna species of the genus Thunnus, such as the bluefin tunas, are some of the most important and yet most endangered trade fish in the world. Identification of these species in traded forms, however, may be difficult depending on the presentation of the products, which may hamper conservation efforts on trade control. In this paper, we validated a genetic methodology that can fully distinguish between the eight Thunnus species from any kind of processed tissue. Methodology: After testing several genetic markers, a complete discrimination of the eight tuna species was achieved using Forensically Informative Nucleotide Sequencing based primarily on the sequence variability of the hypervariable genetic marker mitochondrial DNA control region (mtDNA CR), followed, in some specific cases, by a second validation by a nuclear marker rDNA first internal transcribed spacer (ITS1). This methodology was able to distinguish all tuna species, including those belonging to the subgenus Neothunnus that are very closely related, and in consequence can not be differentiated with other genetic markers of lower variability. This methodology also took into consideration the presence of introgression that has been reported in past studies between T. thynnus, T. orientalis and T. alalunga. Finally, we applied the methodology to cross-check the species identity of 26 processed tuna samples. Conclusions: Using the combination of two genetic markers, one mitochondrial and another nuclear, allows a full discrimination between all eight tuna species. Unexpectedly, the genetic marker traditionally used for DNA barcoding, cytochrome oxidase 1, could not differentiate all species, thus its use as a genetic marker for tuna species identification is questionedPublic Library of Science2009info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10256/3594http://hdl.handle.net/10256/3594PLoS ONE, 2009, vol.4, núm.10, p. e7606Articles publicats (D-B)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0007606info:eu-repo/semantics/altIdentifier/eissn/1932-6203Aquest document està subjecte a una llicència Creative Commons: Reconeixement (by)http://creativecommons.org/licenses/by/2.5/es/deed.cainfo:eu-repo/semantics/openAccessoai:recercat.cat:10256/35942026-05-29T05:05:01Z
dc.title.none.fl_str_mv A Validated Methodology for Genetic Identification of Tuna Species (Genus Thunnus)
title A Validated Methodology for Genetic Identification of Tuna Species (Genus Thunnus)
spellingShingle A Validated Methodology for Genetic Identification of Tuna Species (Genus Thunnus)
Viñas de Puig, Jordi
Tonyina -- Genètica
Tuna -- Genetics
title_short A Validated Methodology for Genetic Identification of Tuna Species (Genus Thunnus)
title_full A Validated Methodology for Genetic Identification of Tuna Species (Genus Thunnus)
title_fullStr A Validated Methodology for Genetic Identification of Tuna Species (Genus Thunnus)
title_full_unstemmed A Validated Methodology for Genetic Identification of Tuna Species (Genus Thunnus)
title_sort A Validated Methodology for Genetic Identification of Tuna Species (Genus Thunnus)
dc.creator.none.fl_str_mv Viñas de Puig, Jordi
Tudela, Sergi
author Viñas de Puig, Jordi
author_facet Viñas de Puig, Jordi
Tudela, Sergi
author_role author
author2 Tudela, Sergi
author2_role author
dc.subject.none.fl_str_mv Tonyina -- Genètica
Tuna -- Genetics
topic Tonyina -- Genètica
Tuna -- Genetics
description Tuna species of the genus Thunnus, such as the bluefin tunas, are some of the most important and yet most endangered trade fish in the world. Identification of these species in traded forms, however, may be difficult depending on the presentation of the products, which may hamper conservation efforts on trade control. In this paper, we validated a genetic methodology that can fully distinguish between the eight Thunnus species from any kind of processed tissue. Methodology: After testing several genetic markers, a complete discrimination of the eight tuna species was achieved using Forensically Informative Nucleotide Sequencing based primarily on the sequence variability of the hypervariable genetic marker mitochondrial DNA control region (mtDNA CR), followed, in some specific cases, by a second validation by a nuclear marker rDNA first internal transcribed spacer (ITS1). This methodology was able to distinguish all tuna species, including those belonging to the subgenus Neothunnus that are very closely related, and in consequence can not be differentiated with other genetic markers of lower variability. This methodology also took into consideration the presence of introgression that has been reported in past studies between T. thynnus, T. orientalis and T. alalunga. Finally, we applied the methodology to cross-check the species identity of 26 processed tuna samples. Conclusions: Using the combination of two genetic markers, one mitochondrial and another nuclear, allows a full discrimination between all eight tuna species. Unexpectedly, the genetic marker traditionally used for DNA barcoding, cytochrome oxidase 1, could not differentiate all species, thus its use as a genetic marker for tuna species identification is questioned
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/3594
http://hdl.handle.net/10256/3594
url http://hdl.handle.net/10256/3594
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0007606
info:eu-repo/semantics/altIdentifier/eissn/1932-6203
dc.rights.none.fl_str_mv Aquest document està subjecte a una llicència Creative Commons: Reconeixement (by)
http://creativecommons.org/licenses/by/2.5/es/deed.ca
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Aquest document està subjecte a una llicència Creative Commons: Reconeixement (by)
http://creativecommons.org/licenses/by/2.5/es/deed.ca
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv PLoS ONE, 2009, vol.4, núm.10, p. e7606
Articles publicats (D-B)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
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