Super-Resolution Microscopy to Study Interorganelle Contact Sites

Interorganelle membrane contact sites (MCS) are areas of close vicinity between the membranes of two organelles that are maintained by protein tethers. Recently, a significant research effort has been made to study MCS, as they are implicated in a wide range of biological functions, such as organell...

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Detalles Bibliográficos
Autores: Nieto Garai, Jon Ander, Olazar Intxausti, June, Anso, Itxaso, Lorizate Nogales, Maier, Terrones Urio, Oihana, Contreras, F. Xabier
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/58909
Acceso en línea:http://hdl.handle.net/10810/58909
Access Level:acceso abierto
Palabra clave:super-resolution microscopy
SIM
TIRFM
STED
SMLM
PALM
STORM
organelles
membrane contact sites
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spelling Super-Resolution Microscopy to Study Interorganelle Contact SitesNieto Garai, Jon AnderOlazar Intxausti, JuneAnso, ItxasoLorizate Nogales, MaierTerrones Urio, OihanaContreras, F. Xabiersuper-resolution microscopySIMTIRFMSTEDSMLMPALMSTORMorganellesmembrane contact sitesInterorganelle membrane contact sites (MCS) are areas of close vicinity between the membranes of two organelles that are maintained by protein tethers. Recently, a significant research effort has been made to study MCS, as they are implicated in a wide range of biological functions, such as organelle biogenesis and division, apoptosis, autophagy, and ion and phospholipid homeostasis. Their composition, characteristics, and dynamics can be studied by different techniques, but in recent years super-resolution fluorescence microscopy (SRFM) has emerged as a powerful tool for studying MCS. In this review, we first explore the main characteristics and biological functions of MCS and summarize the different approaches for studying them. Then, we center on SRFM techniques that have been used to study MCS. For each of the approaches, we summarize their working principle, discuss their advantages and limitations, and explore the main discoveries they have uncovered in the field of MCS.The APC was funded by grant PID2020-117405GBI00, funded by MCIN/AEI/10.13039/501100011033 and, as appropriate, by “ERDF A way of making Europe” by the “European Union” or by the “European Union NextGenerationEU/PRTR”; by the Basque Government, grant numbers ELKARTEK22/86 and IT1625-22; and by Fundación Ramón Areces, grant number CIVP20S11276.MDPI2022202220222022info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/58909reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MICINN/PID2020-117405GBI00/https://www.mdpi.com/1422-0067/23/23/15354info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/© 2022 by the authors.Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).oai:addi.ehu.eus:10810/589092026-06-18T09:23:17Z
dc.title.none.fl_str_mv Super-Resolution Microscopy to Study Interorganelle Contact Sites
title Super-Resolution Microscopy to Study Interorganelle Contact Sites
spellingShingle Super-Resolution Microscopy to Study Interorganelle Contact Sites
Nieto Garai, Jon Ander
super-resolution microscopy
SIM
TIRFM
STED
SMLM
PALM
STORM
organelles
membrane contact sites
title_short Super-Resolution Microscopy to Study Interorganelle Contact Sites
title_full Super-Resolution Microscopy to Study Interorganelle Contact Sites
title_fullStr Super-Resolution Microscopy to Study Interorganelle Contact Sites
title_full_unstemmed Super-Resolution Microscopy to Study Interorganelle Contact Sites
title_sort Super-Resolution Microscopy to Study Interorganelle Contact Sites
dc.creator.none.fl_str_mv Nieto Garai, Jon Ander
Olazar Intxausti, June
Anso, Itxaso
Lorizate Nogales, Maier
Terrones Urio, Oihana
Contreras, F. Xabier
author Nieto Garai, Jon Ander
author_facet Nieto Garai, Jon Ander
Olazar Intxausti, June
Anso, Itxaso
Lorizate Nogales, Maier
Terrones Urio, Oihana
Contreras, F. Xabier
author_role author
author2 Olazar Intxausti, June
Anso, Itxaso
Lorizate Nogales, Maier
Terrones Urio, Oihana
Contreras, F. Xabier
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv super-resolution microscopy
SIM
TIRFM
STED
SMLM
PALM
STORM
organelles
membrane contact sites
topic super-resolution microscopy
SIM
TIRFM
STED
SMLM
PALM
STORM
organelles
membrane contact sites
description Interorganelle membrane contact sites (MCS) are areas of close vicinity between the membranes of two organelles that are maintained by protein tethers. Recently, a significant research effort has been made to study MCS, as they are implicated in a wide range of biological functions, such as organelle biogenesis and division, apoptosis, autophagy, and ion and phospholipid homeostasis. Their composition, characteristics, and dynamics can be studied by different techniques, but in recent years super-resolution fluorescence microscopy (SRFM) has emerged as a powerful tool for studying MCS. In this review, we first explore the main characteristics and biological functions of MCS and summarize the different approaches for studying them. Then, we center on SRFM techniques that have been used to study MCS. For each of the approaches, we summarize their working principle, discuss their advantages and limitations, and explore the main discoveries they have uncovered in the field of MCS.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/58909
url http://hdl.handle.net/10810/58909
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN/PID2020-117405GBI00/
https://www.mdpi.com/1422-0067/23/23/15354
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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