Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa)

An experimental infection with highly pathogenic avian influenza virus (HPAIV) and low pathogenic avian influenza virus (LPAIV) was carried out in red-legged partridges (Alectoris rufa) in order to study clinical signs, gross and microscopic lesions, and viral distribution in tissues and viral shedd...

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Autores: Bertran, Kateri, Pérez-Ramírez, Elisa, Busquets, Núria, Dolz, Roser, Ramis, Antonio, Darji, Ayub, Abad, Francesc Xavier, Valle, Rosa, Chaves, Aida, Serra Gironella, Joan, Barral, Marta, Höfle, Ursula, Majó, Natàlia
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
Repositorio:IRTA Pubpro. Open Digital Archive
OAI Identifier:oai:repositori.irta.cat:20.500.12327/2999
Acceso en línea:http://hdl.handle.net/20.500.12327/2999
https://doi.org/10.1186/1297-9716-42-24
Access Level:acceso abierto
Palabra clave:619
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spelling Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa)Bertran, KateriPérez-Ramírez, ElisaBusquets, NúriaDolz, RoserRamis, AntonioDarji, AyubAbad, Francesc XavierValle, RosaChaves, AidaSerra Gironella, JoanBarral, MartaHöfle, UrsulaMajó, Natàlia619An experimental infection with highly pathogenic avian influenza virus (HPAIV) and low pathogenic avian influenza virus (LPAIV) was carried out in red-legged partridges (Alectoris rufa) in order to study clinical signs, gross and microscopic lesions, and viral distribution in tissues and viral shedding. Birds were infected with a HPAIV subtype H7N1 (A/Chicken/Italy/5093/1999) and a LPAIV subtype H7N9 (A/Anas crecca/Spain/1460/2008). Uninoculated birds were included as contacts in both groups. In HPAIV infected birds, the first clinical signs were observed at 3 dpi, and mortality started at 4 dpi, reaching 100% at 8 dpi. The presence of viral antigen in tissues and viral shedding were confirmed by immunohistochemistry and quantitative real time RT-PCR (qRRT-PCR), respectively, in all birds infected with HPAIV. However, neither clinical signs nor histopathological findings were observed in LPAIV infected partridges. In addition, only short-term viral shedding together with seroconversion was detected in some LPAIV inoculated animals. The present study demonstrates that the red-legged partridge is highly susceptible to the H7N1 HPAIV strain, causing severe disease, mortality and abundant viral shedding and thus contributing to the spread of a potential local outbreak of this virus. In contrast, our results concerning H7N9 LPAIV suggest that the red-legged partridge is not a reservoir species for this virus.This work was partially funded by the coordinated project FAU2006-00019- C03-00 of the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) and the Ministry of Education and Science of Spain. E. Pérez-Ramírez currently has an I3p fellowship from the Spanish Science Council (CSIC), and K. Bertran has an FPU Pre-Doctoral grant from the Ministry of Education of Spain. We are grateful to the personnel of “El Bonillo” farm who kindly provided the animals used in the study. We also thank the personnel of the experimental farm of IREC “Dehesa de Galiana” for their collaboration in bird maintenance, and the personnel of the BSL-3 of CReSA for their excellent technical assistance.info:eu-repo/semantics/publishedVersionBMCProducció AnimalSanitat Animal202420242011info:eu-repo/semantics/article9application/pdfhttp://hdl.handle.net/20.500.12327/2999https://doi.org/10.1186/1297-9716-42-24reponame:IRTA Pubpro. Open Digital Archiveinstname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)InglésVeterinary ResearchINIA/Programa nacional de recursos y tecnologías agroalimentarias/FAU2006-00019-C03-01/ES/Ensayos de persistencia ambiental del virus y estudio de la inmunopatogenia en aves criadas en regímenes no intensivos/Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.irta.cat:20.500.12327/29992026-06-16T08:51:17Z
dc.title.none.fl_str_mv Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa)
title Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa)
spellingShingle Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa)
Bertran, Kateri
619
title_short Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa)
title_full Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa)
title_fullStr Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa)
title_full_unstemmed Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa)
title_sort Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa)
dc.creator.none.fl_str_mv Bertran, Kateri
Pérez-Ramírez, Elisa
Busquets, Núria
Dolz, Roser
Ramis, Antonio
Darji, Ayub
Abad, Francesc Xavier
Valle, Rosa
Chaves, Aida
Serra Gironella, Joan
Barral, Marta
Höfle, Ursula
Majó, Natàlia
author Bertran, Kateri
author_facet Bertran, Kateri
Pérez-Ramírez, Elisa
Busquets, Núria
Dolz, Roser
Ramis, Antonio
Darji, Ayub
Abad, Francesc Xavier
Valle, Rosa
Chaves, Aida
Serra Gironella, Joan
Barral, Marta
Höfle, Ursula
Majó, Natàlia
author_role author
author2 Pérez-Ramírez, Elisa
Busquets, Núria
Dolz, Roser
Ramis, Antonio
Darji, Ayub
Abad, Francesc Xavier
Valle, Rosa
Chaves, Aida
Serra Gironella, Joan
Barral, Marta
Höfle, Ursula
Majó, Natàlia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Sanitat Animal
dc.subject.none.fl_str_mv 619
topic 619
description An experimental infection with highly pathogenic avian influenza virus (HPAIV) and low pathogenic avian influenza virus (LPAIV) was carried out in red-legged partridges (Alectoris rufa) in order to study clinical signs, gross and microscopic lesions, and viral distribution in tissues and viral shedding. Birds were infected with a HPAIV subtype H7N1 (A/Chicken/Italy/5093/1999) and a LPAIV subtype H7N9 (A/Anas crecca/Spain/1460/2008). Uninoculated birds were included as contacts in both groups. In HPAIV infected birds, the first clinical signs were observed at 3 dpi, and mortality started at 4 dpi, reaching 100% at 8 dpi. The presence of viral antigen in tissues and viral shedding were confirmed by immunohistochemistry and quantitative real time RT-PCR (qRRT-PCR), respectively, in all birds infected with HPAIV. However, neither clinical signs nor histopathological findings were observed in LPAIV infected partridges. In addition, only short-term viral shedding together with seroconversion was detected in some LPAIV inoculated animals. The present study demonstrates that the red-legged partridge is highly susceptible to the H7N1 HPAIV strain, causing severe disease, mortality and abundant viral shedding and thus contributing to the spread of a potential local outbreak of this virus. In contrast, our results concerning H7N9 LPAIV suggest that the red-legged partridge is not a reservoir species for this virus.
publishDate 2011
dc.date.none.fl_str_mv 2011
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/2999
https://doi.org/10.1186/1297-9716-42-24
url http://hdl.handle.net/20.500.12327/2999
https://doi.org/10.1186/1297-9716-42-24
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Veterinary Research
INIA/Programa nacional de recursos y tecnologías agroalimentarias/FAU2006-00019-C03-01/ES/Ensayos de persistencia ambiental del virus y estudio de la inmunopatogenia en aves criadas en regímenes no intensivos/
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9
application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:IRTA Pubpro. Open Digital Archive
instname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
instname_str Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
reponame_str IRTA Pubpro. Open Digital Archive
collection IRTA Pubpro. Open Digital Archive
repository.name.fl_str_mv
repository.mail.fl_str_mv
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