Stress relaxation in epithelial monolayers is controlled by the actomyosin cortex

Epithelial monolayers are one-cell thick tissue sheets that separate internal and external environments. As part of their function, they have to withstand extrinsic mechanical stresses applied at high strain rates. However, little is known about how monolayers respond to mechanical deformations. Her...

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Autores: Khalilgharibi, Nargess, Fouchard, Jonathan, Asadipour, Nina, Barrientos, Ricardo, Duda, Maria, Bonfanti, Alessandra, Yonis, Amina, Harris, Andrew, Mosaffa, Payman|||0000-0003-2128-7584, Fujita, Yasuyuki, Kabla, Alexandre, Mao, Yanlan, Baum, Buzz, Muñoz Romero, José|||0000-0002-0083-3673, Miodownik, Mark, Charras, Guillaume
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/174696
Acceso en línea:https://hdl.handle.net/2117/174696
https://dx.doi.org/10.1038/s41567-019-0516-6
Access Level:acceso abierto
Palabra clave:Numerical calculations
Càlculs numèrics
Àrees temàtiques de la UPC::Matemàtiques i estadística::Matemàtica aplicada a les ciències
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spelling Stress relaxation in epithelial monolayers is controlled by the actomyosin cortexKhalilgharibi, NargessFouchard, JonathanAsadipour, NinaBarrientos, RicardoDuda, MariaBonfanti, AlessandraYonis, AminaHarris, AndrewMosaffa, Payman|||0000-0003-2128-7584Fujita, YasuyukiKabla, AlexandreMao, YanlanBaum, BuzzMuñoz Romero, José|||0000-0002-0083-3673Miodownik, MarkCharras, GuillaumeNumerical calculationsCàlculs numèricsÀrees temàtiques de la UPC::Matemàtiques i estadística::Matemàtica aplicada a les ciènciesEpithelial monolayers are one-cell thick tissue sheets that separate internal and external environments. As part of their function, they have to withstand extrinsic mechanical stresses applied at high strain rates. However, little is known about how monolayers respond to mechanical deformations. Here, by subjecting suspended epithelial monolayers to stretch, we find that they dissipate stresses on a minute time-scale in a process that involves an increase in monolayer length, pointing to active remodelling of cell architecture during relaxation. Strikingly, monolayers consisting of tens of thousands of cells relax stress with similar dynamics to single rounded cells and both respond similarly to perturbations of actomyosin. By contrast, cell-cell junctional complexes and intermediate filaments do not relax tissue stress, but form stable connections between cells, allowing monolayers to behave rheologically as single cells. Taken together our data show that actomyosin dynamics governs the rheological properties of epithelial monolayers, dissipating applied stresses, and enabling changes in monolayer length.Peer Reviewed20192019-01-0120202020-01-13journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/174696https://dx.doi.org/10.1038/s41567-019-0516-6reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)InglésengEuropean Commission http://doi.org/10.13039/100010661 Horizon 2020 Framework Programme 647186 Molecular and cellular determinants of cell monolayer mechanicsEuropean Commission http://dx.doi.org/10.13039/100011102 Seventh Framework Programme 240487 Predictive models and simulations in nano- and biomolecular mechanics: a multiscale approachopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/1746962026-05-27T15:37:01Z
dc.title.none.fl_str_mv Stress relaxation in epithelial monolayers is controlled by the actomyosin cortex
title Stress relaxation in epithelial monolayers is controlled by the actomyosin cortex
spellingShingle Stress relaxation in epithelial monolayers is controlled by the actomyosin cortex
Khalilgharibi, Nargess
Numerical calculations
Càlculs numèrics
Àrees temàtiques de la UPC::Matemàtiques i estadística::Matemàtica aplicada a les ciències
title_short Stress relaxation in epithelial monolayers is controlled by the actomyosin cortex
title_full Stress relaxation in epithelial monolayers is controlled by the actomyosin cortex
title_fullStr Stress relaxation in epithelial monolayers is controlled by the actomyosin cortex
title_full_unstemmed Stress relaxation in epithelial monolayers is controlled by the actomyosin cortex
title_sort Stress relaxation in epithelial monolayers is controlled by the actomyosin cortex
dc.creator.none.fl_str_mv Khalilgharibi, Nargess
Fouchard, Jonathan
Asadipour, Nina
Barrientos, Ricardo
Duda, Maria
Bonfanti, Alessandra
Yonis, Amina
Harris, Andrew
Mosaffa, Payman|||0000-0003-2128-7584
Fujita, Yasuyuki
Kabla, Alexandre
Mao, Yanlan
Baum, Buzz
Muñoz Romero, José|||0000-0002-0083-3673
Miodownik, Mark
Charras, Guillaume
author Khalilgharibi, Nargess
author_facet Khalilgharibi, Nargess
Fouchard, Jonathan
Asadipour, Nina
Barrientos, Ricardo
Duda, Maria
Bonfanti, Alessandra
Yonis, Amina
Harris, Andrew
Mosaffa, Payman|||0000-0003-2128-7584
Fujita, Yasuyuki
Kabla, Alexandre
Mao, Yanlan
Baum, Buzz
Muñoz Romero, José|||0000-0002-0083-3673
Miodownik, Mark
Charras, Guillaume
author_role author
author2 Fouchard, Jonathan
Asadipour, Nina
Barrientos, Ricardo
Duda, Maria
Bonfanti, Alessandra
Yonis, Amina
Harris, Andrew
Mosaffa, Payman|||0000-0003-2128-7584
Fujita, Yasuyuki
Kabla, Alexandre
Mao, Yanlan
Baum, Buzz
Muñoz Romero, José|||0000-0002-0083-3673
Miodownik, Mark
Charras, Guillaume
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Numerical calculations
Càlculs numèrics
Àrees temàtiques de la UPC::Matemàtiques i estadística::Matemàtica aplicada a les ciències
topic Numerical calculations
Càlculs numèrics
Àrees temàtiques de la UPC::Matemàtiques i estadística::Matemàtica aplicada a les ciències
description Epithelial monolayers are one-cell thick tissue sheets that separate internal and external environments. As part of their function, they have to withstand extrinsic mechanical stresses applied at high strain rates. However, little is known about how monolayers respond to mechanical deformations. Here, by subjecting suspended epithelial monolayers to stretch, we find that they dissipate stresses on a minute time-scale in a process that involves an increase in monolayer length, pointing to active remodelling of cell architecture during relaxation. Strikingly, monolayers consisting of tens of thousands of cells relax stress with similar dynamics to single rounded cells and both respond similarly to perturbations of actomyosin. By contrast, cell-cell junctional complexes and intermediate filaments do not relax tissue stress, but form stable connections between cells, allowing monolayers to behave rheologically as single cells. Taken together our data show that actomyosin dynamics governs the rheological properties of epithelial monolayers, dissipating applied stresses, and enabling changes in monolayer length.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01
2020
2020-01-13
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/174696
https://dx.doi.org/10.1038/s41567-019-0516-6
url https://hdl.handle.net/2117/174696
https://dx.doi.org/10.1038/s41567-019-0516-6
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission http://doi.org/10.13039/100010661 Horizon 2020 Framework Programme 647186 Molecular and cellular determinants of cell monolayer mechanics
European Commission http://dx.doi.org/10.13039/100011102 Seventh Framework Programme 240487 Predictive models and simulations in nano- and biomolecular mechanics: a multiscale approach
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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