Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally

Ovine enzootic abortion, caused by Chlamydia abortus, leads to important economic losses worldwide. In addition to reproductive failures, infection may impact lamb growth during the first weeks after birth, yet this effect has not been well characterized. Vaccination can help to control the disease...

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Authors: Pérez Sancho, Marta, Díez Guerrier, Alberto Antoine, Salinas, Jesús, Navarro Gómez, Alejandro, García Benzaquén, Nerea, Pozo Piñol, Pilar, Goyache Goñi, Joaquín, Álvarez, Julio, Domínguez Rodríguez, Lucas José, García-Seco Romero, María Teresa
Format: article
Publication Date:2016
Country:España
Institution:Universidad Complutense de Madrid (UCM)
Repository:Docta Complutense
Language:English
OAI Identifier:oai:docta.ucm.es:20.500.14352/24671
Online Access:https://hdl.handle.net/20.500.14352/24671
Access Level:Open access
Keyword:Chlamydia abortus
enzootic abortion
vaccination
challenge
sheep
offspring development
Veterinaria
3109 Ciencias Veterinarias
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spelling Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged ExperimentallyPérez Sancho, MartaDíez Guerrier, Alberto AntoineSalinas, JesúsNavarro Gómez, AlejandroGarcía Benzaquén, NereaPozo Piñol, PilarGoyache Goñi, JoaquínÁlvarez, JulioDomínguez Rodríguez, Lucas JoséGarcía-Seco Romero, María TeresaChlamydia abortusenzootic abortionvaccinationchallengesheepoffspring developmentVeterinaria3109 Ciencias VeterinariasOvine enzootic abortion, caused by Chlamydia abortus, leads to important economic losses worldwide. In addition to reproductive failures, infection may impact lamb growth during the first weeks after birth, yet this effect has not been well characterized. Vaccination can help to control the disease but variable efficacy values have been described, possibly related with factors associated with the host, the vaccine, the parameter used for efficacy determination and the challenge conditions. In this context, we evaluated the efficacy of an inactivated standard commercial vaccine and a 1/2 diluted dose in pregnant sheep challenged with C. abortus by examining multiple indicators ofvaccine effect (including incidence of reproductive failures, bacterial excretion, and evolution of weight gain of viable lambs during the first month of life). Three groups of ewes [control non-vaccinated, C (n = 18); vaccinated with standard dose, SV (n = 16) and vaccinated with 1/2 dose, DV (n = 17)], were challenged approximately 90 days post-mating and tested using direct PCR (tissue samples and vaginal swabs) and ELISA (serum) until 31 days post-reproductive outcome. There were not significant differences in the proportions of reproductive failures or bacterial shedding after birth/abortion regardless the vaccination protocol. However, a beneficial effect of vaccination on offspring growth was detected in both vaccinated groups compared with the controls, with a mean increase in weight measured at 30 days of life of 1.5 and 2.5 Kg (p = 0.056) and an increase in the geometric mean of the daily gain of 8.4 and 9.7% in lambs born from DV and SV ewes compared to controls, respectively. Our results demonstrate the effect of an inactivated vaccine in the development of the offspring of C. abortus-infected ewes at a standard and a diluted dose, an interesting finding given the difficulty in achieving sufficient antigen concentration in the production of EAE-commercial vaccines.Frontiers MediaUniversidad Complutense de Madrid20162016-01-0120162016-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/24671reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/246712026-06-02T12:44:21Z
dc.title.none.fl_str_mv Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally
title Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally
spellingShingle Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally
Pérez Sancho, Marta
Chlamydia abortus
enzootic abortion
vaccination
challenge
sheep
offspring development
Veterinaria
3109 Ciencias Veterinarias
title_short Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally
title_full Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally
title_fullStr Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally
title_full_unstemmed Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally
title_sort Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally
dc.creator.none.fl_str_mv Pérez Sancho, Marta
Díez Guerrier, Alberto Antoine
Salinas, Jesús
Navarro Gómez, Alejandro
García Benzaquén, Nerea
Pozo Piñol, Pilar
Goyache Goñi, Joaquín
Álvarez, Julio
Domínguez Rodríguez, Lucas José
García-Seco Romero, María Teresa
author Pérez Sancho, Marta
author_facet Pérez Sancho, Marta
Díez Guerrier, Alberto Antoine
Salinas, Jesús
Navarro Gómez, Alejandro
García Benzaquén, Nerea
Pozo Piñol, Pilar
Goyache Goñi, Joaquín
Álvarez, Julio
Domínguez Rodríguez, Lucas José
García-Seco Romero, María Teresa
author_role author
author2 Díez Guerrier, Alberto Antoine
Salinas, Jesús
Navarro Gómez, Alejandro
García Benzaquén, Nerea
Pozo Piñol, Pilar
Goyache Goñi, Joaquín
Álvarez, Julio
Domínguez Rodríguez, Lucas José
García-Seco Romero, María Teresa
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv Chlamydia abortus
enzootic abortion
vaccination
challenge
sheep
offspring development
Veterinaria
3109 Ciencias Veterinarias
topic Chlamydia abortus
enzootic abortion
vaccination
challenge
sheep
offspring development
Veterinaria
3109 Ciencias Veterinarias
description Ovine enzootic abortion, caused by Chlamydia abortus, leads to important economic losses worldwide. In addition to reproductive failures, infection may impact lamb growth during the first weeks after birth, yet this effect has not been well characterized. Vaccination can help to control the disease but variable efficacy values have been described, possibly related with factors associated with the host, the vaccine, the parameter used for efficacy determination and the challenge conditions. In this context, we evaluated the efficacy of an inactivated standard commercial vaccine and a 1/2 diluted dose in pregnant sheep challenged with C. abortus by examining multiple indicators ofvaccine effect (including incidence of reproductive failures, bacterial excretion, and evolution of weight gain of viable lambs during the first month of life). Three groups of ewes [control non-vaccinated, C (n = 18); vaccinated with standard dose, SV (n = 16) and vaccinated with 1/2 dose, DV (n = 17)], were challenged approximately 90 days post-mating and tested using direct PCR (tissue samples and vaginal swabs) and ELISA (serum) until 31 days post-reproductive outcome. There were not significant differences in the proportions of reproductive failures or bacterial shedding after birth/abortion regardless the vaccination protocol. However, a beneficial effect of vaccination on offspring growth was detected in both vaccinated groups compared with the controls, with a mean increase in weight measured at 30 days of life of 1.5 and 2.5 Kg (p = 0.056) and an increase in the geometric mean of the daily gain of 8.4 and 9.7% in lambs born from DV and SV ewes compared to controls, respectively. Our results demonstrate the effect of an inactivated vaccine in the development of the offspring of C. abortus-infected ewes at a standard and a diluted dose, an interesting finding given the difficulty in achieving sufficient antigen concentration in the production of EAE-commercial vaccines.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01
2016
2016-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/24671
url https://hdl.handle.net/20.500.14352/24671
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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