Microbiota-Derived Extracellular Vesicles Promote Immunityand Intestinal Maturation in Suckling Rats

<p>Microbiota–host communication is primarily achieved by secreted factors that can penetrate the mucosal surface, such as extracellular membrane vesicles (EVs). The EVs released by the gut microbiota have been extensively studied in cellular and experimental models of human diseases. However,...

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Autores: Gras Miravet, Dunia, Martínez-Ruiz, Sergio, Sáez-Fuertes, Laura, Casanova-Crespo, Sergi, Rodríguez Lagunas, María José, Pérez-Cano, Francisco J., Badía Palacín, Josefa, Baldomà Llavinés, Laura
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/217675
Acceso en línea:https://hdl.handle.net/2445/217675
Access Level:acceso abierto
Palabra clave:Diarrea
Malalties intestinals
Microbiota intestinal
Diarrhea
Intestinal diseases
Gastrointestinal microbiome
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spelling Microbiota-Derived Extracellular Vesicles Promote Immunityand Intestinal Maturation in Suckling RatsGras Miravet, DuniaMartínez-Ruiz, SergioSáez-Fuertes, LauraCasanova-Crespo, SergiRodríguez Lagunas, María JoséPérez-Cano, Francisco J.Badía Palacín, JosefaBaldomà Llavinés, LauraDiarreaMalalties intestinalsMicrobiota intestinalDiarrheaIntestinal diseasesGastrointestinal microbiome<p>Microbiota–host communication is primarily achieved by secreted factors that can penetrate the mucosal surface, such as extracellular membrane vesicles (EVs). The EVs released by the gut microbiota have been extensively studied in cellular and experimental models of human diseases. However, little is known about their in vivo effects in early life, specifically regarding immune and intestinal maturation. This study aimed to investigate the effects of daily administration of EVs from probiotic and commensal E. coli strains in healthy suckling rats during the first 16 days of life. On days 8 and 16, we assessed various intestinal and systemic variables in relation to animal growth, humoral and cellular immunity, epithelial barrier maturation, and intestinal architecture. On day 16, animals given probiotic/microbiota EVs exhibited higher levels of plasma IgG, IgA, and IgM and a greater proportion of Tc, NK, and NKT cells in the spleen. In the small intestine, EVs increased</p><p>the villi area and modulated the expression of genes related to immune function, inflammation, and intestinal permeability, shifting towards an anti-inflammatory and barrier protective profile from day 8. In conclusion, interventions involving probiotic/microbiota EVs may represent a safe postbiotic strategy to stimulate immunity and intestinal maturation in early life.</p>MDPI2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/217675Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/nu15214701Nutrients, 2023, vol. 15, p. 4701https://doi.org/10.3390/nu15214701cc-by (c) Martínez-Ruiz, S. et al., 2023http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2176752026-05-27T06:46:51Z
dc.title.none.fl_str_mv Microbiota-Derived Extracellular Vesicles Promote Immunityand Intestinal Maturation in Suckling Rats
title Microbiota-Derived Extracellular Vesicles Promote Immunityand Intestinal Maturation in Suckling Rats
spellingShingle Microbiota-Derived Extracellular Vesicles Promote Immunityand Intestinal Maturation in Suckling Rats
Gras Miravet, Dunia
Diarrea
Malalties intestinals
Microbiota intestinal
Diarrhea
Intestinal diseases
Gastrointestinal microbiome
title_short Microbiota-Derived Extracellular Vesicles Promote Immunityand Intestinal Maturation in Suckling Rats
title_full Microbiota-Derived Extracellular Vesicles Promote Immunityand Intestinal Maturation in Suckling Rats
title_fullStr Microbiota-Derived Extracellular Vesicles Promote Immunityand Intestinal Maturation in Suckling Rats
title_full_unstemmed Microbiota-Derived Extracellular Vesicles Promote Immunityand Intestinal Maturation in Suckling Rats
title_sort Microbiota-Derived Extracellular Vesicles Promote Immunityand Intestinal Maturation in Suckling Rats
dc.creator.none.fl_str_mv Gras Miravet, Dunia
Martínez-Ruiz, Sergio
Sáez-Fuertes, Laura
Casanova-Crespo, Sergi
Rodríguez Lagunas, María José
Pérez-Cano, Francisco J.
Badía Palacín, Josefa
Baldomà Llavinés, Laura
author Gras Miravet, Dunia
author_facet Gras Miravet, Dunia
Martínez-Ruiz, Sergio
Sáez-Fuertes, Laura
Casanova-Crespo, Sergi
Rodríguez Lagunas, María José
Pérez-Cano, Francisco J.
Badía Palacín, Josefa
Baldomà Llavinés, Laura
author_role author
author2 Martínez-Ruiz, Sergio
Sáez-Fuertes, Laura
Casanova-Crespo, Sergi
Rodríguez Lagunas, María José
Pérez-Cano, Francisco J.
Badía Palacín, Josefa
Baldomà Llavinés, Laura
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Diarrea
Malalties intestinals
Microbiota intestinal
Diarrhea
Intestinal diseases
Gastrointestinal microbiome
topic Diarrea
Malalties intestinals
Microbiota intestinal
Diarrhea
Intestinal diseases
Gastrointestinal microbiome
description <p>Microbiota–host communication is primarily achieved by secreted factors that can penetrate the mucosal surface, such as extracellular membrane vesicles (EVs). The EVs released by the gut microbiota have been extensively studied in cellular and experimental models of human diseases. However, little is known about their in vivo effects in early life, specifically regarding immune and intestinal maturation. This study aimed to investigate the effects of daily administration of EVs from probiotic and commensal E. coli strains in healthy suckling rats during the first 16 days of life. On days 8 and 16, we assessed various intestinal and systemic variables in relation to animal growth, humoral and cellular immunity, epithelial barrier maturation, and intestinal architecture. On day 16, animals given probiotic/microbiota EVs exhibited higher levels of plasma IgG, IgA, and IgM and a greater proportion of Tc, NK, and NKT cells in the spleen. In the small intestine, EVs increased</p><p>the villi area and modulated the expression of genes related to immune function, inflammation, and intestinal permeability, shifting towards an anti-inflammatory and barrier protective profile from day 8. In conclusion, interventions involving probiotic/microbiota EVs may represent a safe postbiotic strategy to stimulate immunity and intestinal maturation in early life.</p>
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/217675
url https://hdl.handle.net/2445/217675
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/nu15214701
Nutrients, 2023, vol. 15, p. 4701
https://doi.org/10.3390/nu15214701
dc.rights.none.fl_str_mv cc-by (c) Martínez-Ruiz, S. et al., 2023
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Martínez-Ruiz, S. et al., 2023
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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