Complete Loss of EPCAM Immunoexpression Identifies EPCAM Deletion Carriers in MSH2-Negative Colorectal Neoplasia

The use of epithelial cell adhesion molecule (EPCAM) immunohistochemistry (IHC) is not included in the colorectal cancer (CRC) screening algorithm to detect Lynch syndrome (LS) patients. The aim of the present study was to demonstrate that EPCAM IHC is a useful tool to guide the LS germ-line analysi...

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Autores: Cuatrecasas Freixas, Miriam, Gorostiaga, Iñigo, Riera, Cristina, Saperas, Esteban, Llort, Gemma, Costa, Irmgard, Matias-Guiu, Xavier, 1958-, Carrato, Cristina, Navarro, Matilde, Pineda Riu, Marta, Dueñas, Nuria, Brunet, Joan, Marco, Vicente, Trias Puigsureda, Isabel, Busteros, José Ignacio, Mateu, Gemma, Balaguer Prunés, Francesc, Fernández Figueras, María Teresa, Esteller, Manel, 1968-, Musulén, Eva
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/173916
Acceso en línea:https://hdl.handle.net/2445/173916
Access Level:acceso abierto
Palabra clave:Càncer colorectal
Malalties del còlon
Immunohistoquímica
Pòlips (Patologia)
Colorectal cancer
Colonic diseases
Immunohistochemistry
Polyps (Pathology)
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spelling Complete Loss of EPCAM Immunoexpression Identifies EPCAM Deletion Carriers in MSH2-Negative Colorectal NeoplasiaCuatrecasas Freixas, MiriamGorostiaga, IñigoRiera, CristinaSaperas, EstebanLlort, GemmaCosta, IrmgardMatias-Guiu, Xavier, 1958-Carrato, CristinaNavarro, MatildePineda Riu, MartaDueñas, NuriaBrunet, JoanMarco, VicenteTrias Puigsureda, IsabelBusteros, José IgnacioMateu, GemmaBalaguer Prunés, FrancescFernández Figueras, María TeresaEsteller, Manel, 1968-Musulén, EvaCàncer colorectalMalalties del còlonImmunohistoquímicaPòlips (Patologia)Colorectal cancerColonic diseasesImmunohistochemistryPolyps (Pathology)The use of epithelial cell adhesion molecule (EPCAM) immunohistochemistry (IHC) is not included in the colorectal cancer (CRC) screening algorithm to detect Lynch syndrome (LS) patients. The aim of the present study was to demonstrate that EPCAM IHC is a useful tool to guide the LS germ-line analysis when a loss of MSH2 expression was present. We retrospectively studied MSH2 and EPCAM IHC in a large series of 190 lesions composed of malignant neoplasms (102), precursor lesions of gastrointestinal (71) and extra-gastrointestinal origin (9), and benign neoplasms (8) from different organs of 71 patients suspicious of being LS due to MSH2 alterations. LS was confirmed in 68 patients, 53 with MSH2 mutations and 15 with EPCAM 3'-end deletions. Tissue microarrays were constructed with human normal tissues and their malignant counterparts to assist in the evaluation of EPCAM staining. Among 154 MSH2-negative lesions, 17 were EPCAM-negative, including 10 CRC and 7 colorectal polyps, and 5 of them showed only isolated negative glands. All lesions showing a lack of EPCAM expression belonged to patients with EPCAM 3'-end deletions. EPCAM IHC is a useful screening tool, with 100% specificity to identify LS patients due to EPCAM 3'-end deletions in MSH2-negative CRC and MSH2-negative colorectal polyps.MDPI2021202120202021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion20 p.application/pdfhttps://hdl.handle.net/2445/173916Articles publicats en revistes (Ciències Fisiològiques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/cancers12102803Cancers, 2020, vol. 12(10), num. 2803https://doi.org/10.3390/cancers12102803cc-by (c) Cuatrecasas Freixas, Miriam et al., 2020http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1739162026-05-29T05:05:01Z
dc.title.none.fl_str_mv Complete Loss of EPCAM Immunoexpression Identifies EPCAM Deletion Carriers in MSH2-Negative Colorectal Neoplasia
title Complete Loss of EPCAM Immunoexpression Identifies EPCAM Deletion Carriers in MSH2-Negative Colorectal Neoplasia
spellingShingle Complete Loss of EPCAM Immunoexpression Identifies EPCAM Deletion Carriers in MSH2-Negative Colorectal Neoplasia
Cuatrecasas Freixas, Miriam
Càncer colorectal
Malalties del còlon
Immunohistoquímica
Pòlips (Patologia)
Colorectal cancer
Colonic diseases
Immunohistochemistry
Polyps (Pathology)
title_short Complete Loss of EPCAM Immunoexpression Identifies EPCAM Deletion Carriers in MSH2-Negative Colorectal Neoplasia
title_full Complete Loss of EPCAM Immunoexpression Identifies EPCAM Deletion Carriers in MSH2-Negative Colorectal Neoplasia
title_fullStr Complete Loss of EPCAM Immunoexpression Identifies EPCAM Deletion Carriers in MSH2-Negative Colorectal Neoplasia
title_full_unstemmed Complete Loss of EPCAM Immunoexpression Identifies EPCAM Deletion Carriers in MSH2-Negative Colorectal Neoplasia
title_sort Complete Loss of EPCAM Immunoexpression Identifies EPCAM Deletion Carriers in MSH2-Negative Colorectal Neoplasia
dc.creator.none.fl_str_mv Cuatrecasas Freixas, Miriam
Gorostiaga, Iñigo
Riera, Cristina
Saperas, Esteban
Llort, Gemma
Costa, Irmgard
Matias-Guiu, Xavier, 1958-
Carrato, Cristina
Navarro, Matilde
Pineda Riu, Marta
Dueñas, Nuria
Brunet, Joan
Marco, Vicente
Trias Puigsureda, Isabel
Busteros, José Ignacio
Mateu, Gemma
Balaguer Prunés, Francesc
Fernández Figueras, María Teresa
Esteller, Manel, 1968-
Musulén, Eva
author Cuatrecasas Freixas, Miriam
author_facet Cuatrecasas Freixas, Miriam
Gorostiaga, Iñigo
Riera, Cristina
Saperas, Esteban
Llort, Gemma
Costa, Irmgard
Matias-Guiu, Xavier, 1958-
Carrato, Cristina
Navarro, Matilde
Pineda Riu, Marta
Dueñas, Nuria
Brunet, Joan
Marco, Vicente
Trias Puigsureda, Isabel
Busteros, José Ignacio
Mateu, Gemma
Balaguer Prunés, Francesc
Fernández Figueras, María Teresa
Esteller, Manel, 1968-
Musulén, Eva
author_role author
author2 Gorostiaga, Iñigo
Riera, Cristina
Saperas, Esteban
Llort, Gemma
Costa, Irmgard
Matias-Guiu, Xavier, 1958-
Carrato, Cristina
Navarro, Matilde
Pineda Riu, Marta
Dueñas, Nuria
Brunet, Joan
Marco, Vicente
Trias Puigsureda, Isabel
Busteros, José Ignacio
Mateu, Gemma
Balaguer Prunés, Francesc
Fernández Figueras, María Teresa
Esteller, Manel, 1968-
Musulén, Eva
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Càncer colorectal
Malalties del còlon
Immunohistoquímica
Pòlips (Patologia)
Colorectal cancer
Colonic diseases
Immunohistochemistry
Polyps (Pathology)
topic Càncer colorectal
Malalties del còlon
Immunohistoquímica
Pòlips (Patologia)
Colorectal cancer
Colonic diseases
Immunohistochemistry
Polyps (Pathology)
description The use of epithelial cell adhesion molecule (EPCAM) immunohistochemistry (IHC) is not included in the colorectal cancer (CRC) screening algorithm to detect Lynch syndrome (LS) patients. The aim of the present study was to demonstrate that EPCAM IHC is a useful tool to guide the LS germ-line analysis when a loss of MSH2 expression was present. We retrospectively studied MSH2 and EPCAM IHC in a large series of 190 lesions composed of malignant neoplasms (102), precursor lesions of gastrointestinal (71) and extra-gastrointestinal origin (9), and benign neoplasms (8) from different organs of 71 patients suspicious of being LS due to MSH2 alterations. LS was confirmed in 68 patients, 53 with MSH2 mutations and 15 with EPCAM 3'-end deletions. Tissue microarrays were constructed with human normal tissues and their malignant counterparts to assist in the evaluation of EPCAM staining. Among 154 MSH2-negative lesions, 17 were EPCAM-negative, including 10 CRC and 7 colorectal polyps, and 5 of them showed only isolated negative glands. All lesions showing a lack of EPCAM expression belonged to patients with EPCAM 3'-end deletions. EPCAM IHC is a useful screening tool, with 100% specificity to identify LS patients due to EPCAM 3'-end deletions in MSH2-negative CRC and MSH2-negative colorectal polyps.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/173916
url https://hdl.handle.net/2445/173916
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/cancers12102803
Cancers, 2020, vol. 12(10), num. 2803
https://doi.org/10.3390/cancers12102803
dc.rights.none.fl_str_mv cc-by (c) Cuatrecasas Freixas, Miriam et al., 2020
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Cuatrecasas Freixas, Miriam et al., 2020
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 20 p.
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Fisiològiques)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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