Development and validation of a methodology based on Captiva EMR-lipid clean-up and LC-MS/MS analysis for the simultaneous determination of mycotoxins in human plasma
We report the methodology for the quantification of 19 mycotoxins in human plasma using high performance liquid chromatography-mass spectrometry (triple quadrupole). The studied mycotoxins were: deepoxy-deoxynivalenol, aflatoxins (B1, B2, G1, G2 and M1), T-2 and HT-2, ochratoxins A and B, zearalenon...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/58063 |
| Acceso en línea: | https://hdl.handle.net/10171/58063 |
| Access Level: | acceso abierto |
| Palabra clave: | Mycotoxins Multi-detection Human plasma LC-MS/MS Human biomonitoring |
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Development and validation of a methodology based on Captiva EMR-lipid clean-up and LC-MS/MS analysis for the simultaneous determination of mycotoxins in human plasmaArce-López, B. (Beatriz)|||/items/7a8c3b9b-a592-4167-9042-d6e308b2b562Lizarraga, E. (Elena)|||/items/84976e6e-e72a-45c2-a275-612ce7e38f3fFlores-Flores, M.E. (Myra Evelyn)|||/items/168ac9fb-32d6-48c2-9193-591082874a13Irigoyen-Barrio, A. (Angel)|||/items/2b0c2de8-4355-469d-8514-327f500bbc71Gonzalez-Peñas, E. (Elena)|||/items/0e4b9ed7-4224-4664-a4f1-c6403a8c833fMycotoxinsMulti-detectionHuman plasmaLC-MS/MSHuman biomonitoringWe report the methodology for the quantification of 19 mycotoxins in human plasma using high performance liquid chromatography-mass spectrometry (triple quadrupole). The studied mycotoxins were: deepoxy-deoxynivalenol, aflatoxins (B1, B2, G1, G2 and M1), T-2 and HT-2, ochratoxins A and B, zearalenone, sterigmatocystin, nivalenol, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, neosolaniol, diacetoxyscirpenol and fusarenon-X. Sample deproteinization and cleanup were performed in one step using Captiva EMR-lipid (3 mL) cartridges and acetonitrile (with 1% formic acid). The extraction step was simple and fast. Validation was based on the evaluation of limits of detection (LOD) and quantification, linearity, precision, recovery, matrix effect, and stability. LOD values ranged from 0.04 ng/mL for aflatoxin B1 to 2.7 ng/mL for HT-2, except for nivalenol, which was 9.1 ng/mL. Recovery was obtained in intermediate precision conditions and at three concentration levels. Mean values ranged from 68.8% for sterigmatocystin to 97.6% for diacetoxyscirpenol (RDS ≤ 15% for all the mycotoxins). Matrix effects (assessed at three concentration levels and in intermediate conditions) were not significant for most of the mycotoxins and were between 75.4% for sterigmatocystin and 109.3% for ochratoxin B (RDS ≤ 15% for all the mycotoxins). This methodology will be useful in human biomonitoring studies of mycotoxins for its reliability.ElsevierDadun. Depósito Académico Digital Universidad de Navarra20192019-09-0520202020-01-0120202020-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/58063reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/580632026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Development and validation of a methodology based on Captiva EMR-lipid clean-up and LC-MS/MS analysis for the simultaneous determination of mycotoxins in human plasma |
| title |
Development and validation of a methodology based on Captiva EMR-lipid clean-up and LC-MS/MS analysis for the simultaneous determination of mycotoxins in human plasma |
| spellingShingle |
Development and validation of a methodology based on Captiva EMR-lipid clean-up and LC-MS/MS analysis for the simultaneous determination of mycotoxins in human plasma Arce-López, B. (Beatriz)|||/items/7a8c3b9b-a592-4167-9042-d6e308b2b562 Mycotoxins Multi-detection Human plasma LC-MS/MS Human biomonitoring |
| title_short |
Development and validation of a methodology based on Captiva EMR-lipid clean-up and LC-MS/MS analysis for the simultaneous determination of mycotoxins in human plasma |
| title_full |
Development and validation of a methodology based on Captiva EMR-lipid clean-up and LC-MS/MS analysis for the simultaneous determination of mycotoxins in human plasma |
| title_fullStr |
Development and validation of a methodology based on Captiva EMR-lipid clean-up and LC-MS/MS analysis for the simultaneous determination of mycotoxins in human plasma |
| title_full_unstemmed |
Development and validation of a methodology based on Captiva EMR-lipid clean-up and LC-MS/MS analysis for the simultaneous determination of mycotoxins in human plasma |
| title_sort |
Development and validation of a methodology based on Captiva EMR-lipid clean-up and LC-MS/MS analysis for the simultaneous determination of mycotoxins in human plasma |
| dc.creator.none.fl_str_mv |
Arce-López, B. (Beatriz)|||/items/7a8c3b9b-a592-4167-9042-d6e308b2b562 Lizarraga, E. (Elena)|||/items/84976e6e-e72a-45c2-a275-612ce7e38f3f Flores-Flores, M.E. (Myra Evelyn)|||/items/168ac9fb-32d6-48c2-9193-591082874a13 Irigoyen-Barrio, A. (Angel)|||/items/2b0c2de8-4355-469d-8514-327f500bbc71 Gonzalez-Peñas, E. (Elena)|||/items/0e4b9ed7-4224-4664-a4f1-c6403a8c833f |
| author |
Arce-López, B. (Beatriz)|||/items/7a8c3b9b-a592-4167-9042-d6e308b2b562 |
| author_facet |
Arce-López, B. (Beatriz)|||/items/7a8c3b9b-a592-4167-9042-d6e308b2b562 Lizarraga, E. (Elena)|||/items/84976e6e-e72a-45c2-a275-612ce7e38f3f Flores-Flores, M.E. (Myra Evelyn)|||/items/168ac9fb-32d6-48c2-9193-591082874a13 Irigoyen-Barrio, A. (Angel)|||/items/2b0c2de8-4355-469d-8514-327f500bbc71 Gonzalez-Peñas, E. (Elena)|||/items/0e4b9ed7-4224-4664-a4f1-c6403a8c833f |
| author_role |
author |
| author2 |
Lizarraga, E. (Elena)|||/items/84976e6e-e72a-45c2-a275-612ce7e38f3f Flores-Flores, M.E. (Myra Evelyn)|||/items/168ac9fb-32d6-48c2-9193-591082874a13 Irigoyen-Barrio, A. (Angel)|||/items/2b0c2de8-4355-469d-8514-327f500bbc71 Gonzalez-Peñas, E. (Elena)|||/items/0e4b9ed7-4224-4664-a4f1-c6403a8c833f |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Mycotoxins Multi-detection Human plasma LC-MS/MS Human biomonitoring |
| topic |
Mycotoxins Multi-detection Human plasma LC-MS/MS Human biomonitoring |
| description |
We report the methodology for the quantification of 19 mycotoxins in human plasma using high performance liquid chromatography-mass spectrometry (triple quadrupole). The studied mycotoxins were: deepoxy-deoxynivalenol, aflatoxins (B1, B2, G1, G2 and M1), T-2 and HT-2, ochratoxins A and B, zearalenone, sterigmatocystin, nivalenol, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, neosolaniol, diacetoxyscirpenol and fusarenon-X. Sample deproteinization and cleanup were performed in one step using Captiva EMR-lipid (3 mL) cartridges and acetonitrile (with 1% formic acid). The extraction step was simple and fast. Validation was based on the evaluation of limits of detection (LOD) and quantification, linearity, precision, recovery, matrix effect, and stability. LOD values ranged from 0.04 ng/mL for aflatoxin B1 to 2.7 ng/mL for HT-2, except for nivalenol, which was 9.1 ng/mL. Recovery was obtained in intermediate precision conditions and at three concentration levels. Mean values ranged from 68.8% for sterigmatocystin to 97.6% for diacetoxyscirpenol (RDS ≤ 15% for all the mycotoxins). Matrix effects (assessed at three concentration levels and in intermediate conditions) were not significant for most of the mycotoxins and were between 75.4% for sterigmatocystin and 109.3% for ochratoxin B (RDS ≤ 15% for all the mycotoxins). This methodology will be useful in human biomonitoring studies of mycotoxins for its reliability. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019 2019-09-05 2020 2020-01-01 2020 2020-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/58063 |
| url |
https://hdl.handle.net/10171/58063 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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15,300719 |