Combi-CLEAs of glucose oxidase and catalase for conversion of glucose to gluconic acid eliminating the hydrogen peroxide to maintain enzyme activity in a bubble column reactor

© The Author(s).

Detalles Bibliográficos
Autores: Oliveira Mafra, Agnes Cristina, Gazzotto Ulrich, Letícia, Kornecki, Jakub F., Fernández-Lafuente, Roberto, Tardioli, Paulo W., Perencin de Arruda Ribeiro, Marcelo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/188940
Acceso en línea:http://hdl.handle.net/10261/188940
Access Level:acceso abierto
Palabra clave:Glucose-oxidase
Catalase
Combi-CLEAs
Gluconic acid
Pneumatic reactor
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spelling Combi-CLEAs of glucose oxidase and catalase for conversion of glucose to gluconic acid eliminating the hydrogen peroxide to maintain enzyme activity in a bubble column reactorOliveira Mafra, Agnes CristinaGazzotto Ulrich, LetíciaKornecki, Jakub F.Fernández-Lafuente, RobertoTardioli, Paulo W.Perencin de Arruda Ribeiro, MarceloGlucose-oxidaseCatalaseCombi-CLEAsGluconic acidPneumatic reactor© The Author(s).In this study combined cross-linked aggregates of catalase from bovine liver and glucose-oxidase from Aspergillus niger were prepared, and the effects of the precipitant and crosslinking agents, as well as the use of bovine serum albumin (BSA) as a feeder protein, on enzyme immobilization yield and thermal stability of both enzymes, were evaluated. Combi- crosslinking of enzyme aggregates (CLEAs) prepared using dimethoxyethane as precipitant, 25 mM glutaraldehyde and BSA/enzymes mass ratio of 5.45 (w/w), exhibited the highest enzyme activities and stabilities at 40 °C, pH 6.0, and 250 rpm for 5 h. The stability of both immobilized enzymes was fairly similar, eliminating one of the problems of enzyme coimmobilization. Combi-CLEAs were used in gluconic acid (GA) production in a bubble column reactor operated at 40 °C, pH 6.0 and 10 vvm of aeration, using 26 g L−1 glucose as the substrate. Results showed conversion of around 96% and a reaction course very similar to the same process using free enzymes. The operational half-life was 34 h, determined from kinetic profiles and the first order inactivation model. Combi-CLEAs of glucose-oxidase and catalase were shown to be a robust biocatalyst for applications in the production of gluconic acid from glucose.This work was supported by the National Council for Scientific and Technological Development (CNPq) (grants #141647/2013-2 and #402850/2013-0), and in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior–Brazil (CAPES)–Finance Code 001, and by the Comunidad Autónoma de Madrid (project Ref. IND2017/IND-7640) and MINECO from Spanish Government, (project number CTQ2017-86170-R).Peer reviewedMultidisciplinary Digital Publishing InstituteConselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)Fundaçao Capes (Brasil)Comunidad de MadridMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/188940reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2017-86170-Rhttps://doi.org/10.3390/catal9080657Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1889402026-05-22T06:33:51Z
dc.title.none.fl_str_mv Combi-CLEAs of glucose oxidase and catalase for conversion of glucose to gluconic acid eliminating the hydrogen peroxide to maintain enzyme activity in a bubble column reactor
title Combi-CLEAs of glucose oxidase and catalase for conversion of glucose to gluconic acid eliminating the hydrogen peroxide to maintain enzyme activity in a bubble column reactor
spellingShingle Combi-CLEAs of glucose oxidase and catalase for conversion of glucose to gluconic acid eliminating the hydrogen peroxide to maintain enzyme activity in a bubble column reactor
Oliveira Mafra, Agnes Cristina
Glucose-oxidase
Catalase
Combi-CLEAs
Gluconic acid
Pneumatic reactor
title_short Combi-CLEAs of glucose oxidase and catalase for conversion of glucose to gluconic acid eliminating the hydrogen peroxide to maintain enzyme activity in a bubble column reactor
title_full Combi-CLEAs of glucose oxidase and catalase for conversion of glucose to gluconic acid eliminating the hydrogen peroxide to maintain enzyme activity in a bubble column reactor
title_fullStr Combi-CLEAs of glucose oxidase and catalase for conversion of glucose to gluconic acid eliminating the hydrogen peroxide to maintain enzyme activity in a bubble column reactor
title_full_unstemmed Combi-CLEAs of glucose oxidase and catalase for conversion of glucose to gluconic acid eliminating the hydrogen peroxide to maintain enzyme activity in a bubble column reactor
title_sort Combi-CLEAs of glucose oxidase and catalase for conversion of glucose to gluconic acid eliminating the hydrogen peroxide to maintain enzyme activity in a bubble column reactor
dc.creator.none.fl_str_mv Oliveira Mafra, Agnes Cristina
Gazzotto Ulrich, Letícia
Kornecki, Jakub F.
Fernández-Lafuente, Roberto
Tardioli, Paulo W.
Perencin de Arruda Ribeiro, Marcelo
author Oliveira Mafra, Agnes Cristina
author_facet Oliveira Mafra, Agnes Cristina
Gazzotto Ulrich, Letícia
Kornecki, Jakub F.
Fernández-Lafuente, Roberto
Tardioli, Paulo W.
Perencin de Arruda Ribeiro, Marcelo
author_role author
author2 Gazzotto Ulrich, Letícia
Kornecki, Jakub F.
Fernández-Lafuente, Roberto
Tardioli, Paulo W.
Perencin de Arruda Ribeiro, Marcelo
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)
Fundaçao Capes (Brasil)
Comunidad de Madrid
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Glucose-oxidase
Catalase
Combi-CLEAs
Gluconic acid
Pneumatic reactor
topic Glucose-oxidase
Catalase
Combi-CLEAs
Gluconic acid
Pneumatic reactor
description © The Author(s).
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/188940
url http://hdl.handle.net/10261/188940
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2017-86170-R
https://doi.org/10.3390/catal9080657
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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