Extracellular vesicles from L. paracasei improve neuroinflammation, GABA neurotransmission and motor incoordination in hyperammonemic rats

Patients with liver cirrhosis may show minimal hepatic encephalopathy (MHE) with motor incoordination and cognitive impairment that reduce life quality and span. Motor incoordination is due to neuroinflammation and enhanced GABAergic neurotransmission in cerebellum. Recent reports support that probi...

Full description

Bibliographic Details
Authors: Arenas YM, Pérez-Martinez G, Montoliu C, Llansola M, Felipo V
Format: article
Status:Published version
Publication Date:2025
Country:España
Institution:INCLIVA
Repository:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
OAI Identifier:oai:incliva.fundanetsuite.com:p19012
Online Access:https://incliva.portalinvestigacion.com/publicaciones/19012
Access Level:Open access
Keyword:Lacticaseibacillus paracasei
Extracellular vesicles
Hepatic encephalopathy
Hyperammonemia
Neuroinflammation
Motor incoordination
GABAergic neurotransmission
id ES_af88fbbdd77cf7ffef70b5992ab8a981
oai_identifier_str oai:incliva.fundanetsuite.com:p19012
network_acronym_str ES
network_name_str España
repository_id_str
spelling Extracellular vesicles from L. paracasei improve neuroinflammation, GABA neurotransmission and motor incoordination in hyperammonemic ratsArenas YMPérez-Martinez GMontoliu CLlansola MFelipo VLacticaseibacillus paracaseiExtracellular vesiclesHepatic encephalopathyHyperammonemiaNeuroinflammationMotor incoordinationGABAergic neurotransmissionPatients with liver cirrhosis may show minimal hepatic encephalopathy (MHE) with motor incoordination and cognitive impairment that reduce life quality and span. Motor incoordination is due to neuroinflammation and enhanced GABAergic neurotransmission in cerebellum. Recent reports support that probiotics, including L. casei, may improve cognitive function in different pathologies and MHE in cirrhotic patients. Extracellular vesicles (EV) are small cell-derived membrane vesicles that carry bioactive molecules released from cells, including bacteria. We hypothesized that EV from Lacticaseibacillus paracasei (LC-EV) could improve neuroinflammation, GABAergic neurotransmission and motor function in MHE. We show that LC-EV treatment reverses glial activation and neuroinflammation in cerebellum and restore motor coordination in hyperammonemic rats. Moreover, ex vivo treatment of cerebellar slices from hyperammonemic rats with LC-EV also reverses glial activation and neuroinflammation, and the enhancement of the TNFR1-S1PR2-BDNF-TrkB and TNFR1-TrkB-pAKT-NF kappa Bglutaminase-GAT3 pathways and of GABAergic neurotransmission. The results reported support that LC-EV may be used as a therapeutic tool to improve motor incoordination in patients with MHE.ACADEMIC PRESS INC ELSEVIER SCIENCE2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://incliva.portalinvestigacion.com/publicaciones/19012BRAIN BEHAVIOR AND IMMUNITYISSN: 08891591ISSNe: 10902139reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVAinstname:INCLIVAInglésinfo:eu-repo/semantics/openAccessoai:incliva.fundanetsuite.com:p190122026-06-07T16:35:31Z
dc.title.none.fl_str_mv Extracellular vesicles from L. paracasei improve neuroinflammation, GABA neurotransmission and motor incoordination in hyperammonemic rats
title Extracellular vesicles from L. paracasei improve neuroinflammation, GABA neurotransmission and motor incoordination in hyperammonemic rats
spellingShingle Extracellular vesicles from L. paracasei improve neuroinflammation, GABA neurotransmission and motor incoordination in hyperammonemic rats
Arenas YM
Lacticaseibacillus paracasei
Extracellular vesicles
Hepatic encephalopathy
Hyperammonemia
Neuroinflammation
Motor incoordination
GABAergic neurotransmission
title_short Extracellular vesicles from L. paracasei improve neuroinflammation, GABA neurotransmission and motor incoordination in hyperammonemic rats
title_full Extracellular vesicles from L. paracasei improve neuroinflammation, GABA neurotransmission and motor incoordination in hyperammonemic rats
title_fullStr Extracellular vesicles from L. paracasei improve neuroinflammation, GABA neurotransmission and motor incoordination in hyperammonemic rats
title_full_unstemmed Extracellular vesicles from L. paracasei improve neuroinflammation, GABA neurotransmission and motor incoordination in hyperammonemic rats
title_sort Extracellular vesicles from L. paracasei improve neuroinflammation, GABA neurotransmission and motor incoordination in hyperammonemic rats
dc.creator.none.fl_str_mv Arenas YM
Pérez-Martinez G
Montoliu C
Llansola M
Felipo V
author Arenas YM
author_facet Arenas YM
Pérez-Martinez G
Montoliu C
Llansola M
Felipo V
author_role author
author2 Pérez-Martinez G
Montoliu C
Llansola M
Felipo V
author2_role author
author
author
author
dc.subject.none.fl_str_mv Lacticaseibacillus paracasei
Extracellular vesicles
Hepatic encephalopathy
Hyperammonemia
Neuroinflammation
Motor incoordination
GABAergic neurotransmission
topic Lacticaseibacillus paracasei
Extracellular vesicles
Hepatic encephalopathy
Hyperammonemia
Neuroinflammation
Motor incoordination
GABAergic neurotransmission
description Patients with liver cirrhosis may show minimal hepatic encephalopathy (MHE) with motor incoordination and cognitive impairment that reduce life quality and span. Motor incoordination is due to neuroinflammation and enhanced GABAergic neurotransmission in cerebellum. Recent reports support that probiotics, including L. casei, may improve cognitive function in different pathologies and MHE in cirrhotic patients. Extracellular vesicles (EV) are small cell-derived membrane vesicles that carry bioactive molecules released from cells, including bacteria. We hypothesized that EV from Lacticaseibacillus paracasei (LC-EV) could improve neuroinflammation, GABAergic neurotransmission and motor function in MHE. We show that LC-EV treatment reverses glial activation and neuroinflammation in cerebellum and restore motor coordination in hyperammonemic rats. Moreover, ex vivo treatment of cerebellar slices from hyperammonemic rats with LC-EV also reverses glial activation and neuroinflammation, and the enhancement of the TNFR1-S1PR2-BDNF-TrkB and TNFR1-TrkB-pAKT-NF kappa Bglutaminase-GAT3 pathways and of GABAergic neurotransmission. The results reported support that LC-EV may be used as a therapeutic tool to improve motor incoordination in patients with MHE.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://incliva.portalinvestigacion.com/publicaciones/19012
url https://incliva.portalinvestigacion.com/publicaciones/19012
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ACADEMIC PRESS INC ELSEVIER SCIENCE
publisher.none.fl_str_mv ACADEMIC PRESS INC ELSEVIER SCIENCE
dc.source.none.fl_str_mv BRAIN BEHAVIOR AND IMMUNITY
ISSN: 08891591
ISSNe: 10902139
reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
instname:INCLIVA
instname_str INCLIVA
reponame_str r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
collection r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869416692873953280
score 15.81155

Similar Items