Ecological and genomic features of two widespread freshwater picocyanobacteria

We present two genomes of widespread freshwater picocyanobacteria isolated by extinction dilution from a Spanish oligotrophic reservoir. Based on microscopy and genomic properties, both picocyanobacteria were tentatively designated Synechococcus lacustris Tous, formerly described as a metagenome ass...

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Autores: Cabello Yeves, Pedro J., Picazo, Antonio, Camacho, Antonio, Callieri, Cristiana, Rosselli, Riccardo, Roda Garcia, Juan J., Coutinho, Felipe H., Rodríguez Valera, Francisco, Hess, Wolfgang R.
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad Miguel Hernández de Elche
Repositorio:REDIUMH. Depósito Digital de la UMH
OAI Identifier:oai:dspace.umh.es:11000/5257
Acceso en línea:http://hdl.handle.net/11000/5257
Access Level:acceso abierto
Palabra clave:579 - Microbiología
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spelling Ecological and genomic features of two widespread freshwater picocyanobacteriaCabello Yeves, Pedro J.Picazo, AntonioCamacho, AntonioCallieri, CristianaRosselli, RiccardoRoda Garcia, Juan J.Coutinho, Felipe H.Rodríguez Valera, FranciscoHess, Wolfgang R.579 - MicrobiologíaWe present two genomes of widespread freshwater picocyanobacteria isolated by extinction dilution from a Spanish oligotrophic reservoir. Based on microscopy and genomic properties, both picocyanobacteria were tentatively designated Synechococcus lacustris Tous, formerly described as a metagenome assembled genome (MAG) from the same habitat, and Cyanobium usitatum Tous, described here for the first time. Both strains were purified in unicyanobacterial cultures, and their genomes were sequenced. They are broadly distributed in freshwater systems; the first seems to be a specialist on temperate reservoirs (Tous, Amadorio, Dexter, Lake Lanier, Sparkling), and the second appears to also be abundant in cold environments including ice-covered lakes such as Lake Baikal, Lake Erie or the brackish Baltic Sea. Having complete genomes provided access to the flexible genome that does not assemble in MAGs. We found several genomic islands in both genomes, within which there were genes for nitrogen acquisition, transporters for a wide set of compounds and biosynthesis of phycobilisomes in both strains. Some of these regions of low coverage in metagenomes also included antimicrobial compounds, transposases and phage defence systems, including a novel type III CRISPR-Cas phage defence system that was only detected in Synechococcus lacustris Tous.FR-V was supported by grant‘VIREVO’CGL2016–76273-P[AEI/FEDER, EU], (cofunded with FEDER funds)Acciones de dinamización‘REDES DE EXCELENCIA’CONSOLIDER-CGL2015–71523-REDC from the Spanish Ministerio de Economía, Industria y Competitividad and PROMETEO II/2014/012‘AQUAMET’from Generalitat Valenciana.AC and AP were also supported by the projectCLIMAWET, CGL2015-69557-R’granted to AC and funded by the Spanish Ministry of Economy and Competitiveness,and by the European Union through the European Fund for Regional Development‘One way to make EuropeDepartamentos de la UMH::Producción Vegetal y Microbiología2019201920182019info:eu-repo/semantics/articleapplication/pdf26application/pdfhttp://hdl.handle.net/11000/5257reponame:REDIUMH. Depósito Digital de la UMHinstname:Universidad Miguel Hernández de ElcheIngléshttps://doi.org/10.1111/1462-2920.14377info:eu-repo/semantics/openAccessoai:dspace.umh.es:11000/52572026-05-27T13:36:21Z
dc.title.none.fl_str_mv Ecological and genomic features of two widespread freshwater picocyanobacteria
title Ecological and genomic features of two widespread freshwater picocyanobacteria
spellingShingle Ecological and genomic features of two widespread freshwater picocyanobacteria
Cabello Yeves, Pedro J.
579 - Microbiología
title_short Ecological and genomic features of two widespread freshwater picocyanobacteria
title_full Ecological and genomic features of two widespread freshwater picocyanobacteria
title_fullStr Ecological and genomic features of two widespread freshwater picocyanobacteria
title_full_unstemmed Ecological and genomic features of two widespread freshwater picocyanobacteria
title_sort Ecological and genomic features of two widespread freshwater picocyanobacteria
dc.creator.none.fl_str_mv Cabello Yeves, Pedro J.
Picazo, Antonio
Camacho, Antonio
Callieri, Cristiana
Rosselli, Riccardo
Roda Garcia, Juan J.
Coutinho, Felipe H.
Rodríguez Valera, Francisco
Hess, Wolfgang R.
author Cabello Yeves, Pedro J.
author_facet Cabello Yeves, Pedro J.
Picazo, Antonio
Camacho, Antonio
Callieri, Cristiana
Rosselli, Riccardo
Roda Garcia, Juan J.
Coutinho, Felipe H.
Rodríguez Valera, Francisco
Hess, Wolfgang R.
author_role author
author2 Picazo, Antonio
Camacho, Antonio
Callieri, Cristiana
Rosselli, Riccardo
Roda Garcia, Juan J.
Coutinho, Felipe H.
Rodríguez Valera, Francisco
Hess, Wolfgang R.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamentos de la UMH::Producción Vegetal y Microbiología
dc.subject.none.fl_str_mv 579 - Microbiología
topic 579 - Microbiología
description We present two genomes of widespread freshwater picocyanobacteria isolated by extinction dilution from a Spanish oligotrophic reservoir. Based on microscopy and genomic properties, both picocyanobacteria were tentatively designated Synechococcus lacustris Tous, formerly described as a metagenome assembled genome (MAG) from the same habitat, and Cyanobium usitatum Tous, described here for the first time. Both strains were purified in unicyanobacterial cultures, and their genomes were sequenced. They are broadly distributed in freshwater systems; the first seems to be a specialist on temperate reservoirs (Tous, Amadorio, Dexter, Lake Lanier, Sparkling), and the second appears to also be abundant in cold environments including ice-covered lakes such as Lake Baikal, Lake Erie or the brackish Baltic Sea. Having complete genomes provided access to the flexible genome that does not assemble in MAGs. We found several genomic islands in both genomes, within which there were genes for nitrogen acquisition, transporters for a wide set of compounds and biosynthesis of phycobilisomes in both strains. Some of these regions of low coverage in metagenomes also included antimicrobial compounds, transposases and phage defence systems, including a novel type III CRISPR-Cas phage defence system that was only detected in Synechococcus lacustris Tous.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/11000/5257
url http://hdl.handle.net/11000/5257
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1111/1462-2920.14377
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
26
application/pdf
dc.source.none.fl_str_mv reponame:REDIUMH. Depósito Digital de la UMH
instname:Universidad Miguel Hernández de Elche
instname_str Universidad Miguel Hernández de Elche
reponame_str REDIUMH. Depósito Digital de la UMH
collection REDIUMH. Depósito Digital de la UMH
repository.name.fl_str_mv
repository.mail.fl_str_mv
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