Insights into the light response of Skeletonema marinoi: Involvement of ovothiol

This article belongs to the Special Issue Sulfur-Containing Marine Bioactives.

Bibliographic Details
Authors: Milito, Alfonsina, Orefice, Ida, Smerilli, Arianna, Castellano, Immacolata, Napolitano, Alessandra, Brunet, Christophe, Palumbo, Anna
Format: article
Status:Published version
Publication Date:2020
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/229186
Online Access:http://hdl.handle.net/10261/229186
Access Level:Open access
Keyword:Algae
Ovothiol
Antioxidants
Diatoms
Light
Nitric oxide
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repository_id_str
spelling Insights into the light response of Skeletonema marinoi: Involvement of ovothiolMilito, AlfonsinaOrefice, IdaSmerilli, AriannaCastellano, ImmacolataNapolitano, AlessandraBrunet, ChristophePalumbo, AnnaAlgaeOvothiolAntioxidantsDiatomsLightNitric oxideThis article belongs to the Special Issue Sulfur-Containing Marine Bioactives.Diatoms are one of the most widespread groups of microalgae on Earth. They possess extraordinary metabolic capabilities, including a great ability to adapt to different light conditions. Recently, we have discovered that the diatom Skeletonema marinoi produces the natural antioxidant ovothiol B, until then identified only in clams. In this study, we investigated the light-dependent modulation of ovothiol biosynthesis in S. marinoi. Diatoms were exposed to different light conditions, ranging from prolonged darkness to low or high light, also differing in the velocity of intensity increase (sinusoidal versus square-wave distribution). The expression of the gene encoding the key ovothiol biosynthetic enzyme, ovoA, was upregulated by high sinusoidal light mimicking natural conditions. Under this situation higher levels of reactive oxygen species and nitric oxide as well as ovothiol and glutathione increase were detected. No ovoA modulation was observed under prolonged darkness nor low sinusoidal light. Unnatural conditions such as continuous square-wave light induced a very high oxidative stress leading to a drop in cell growth, without enhancing ovoA gene expression. Only one of the inducible forms of nitric oxide synthase, nos2, was upregulated by light with consequent production of NO under sinusoidal light and darkness conditions. Our data suggest that ovothiol biosynthesis is triggered by a combined light stress caused by natural distribution and increased photon flux density, with no influence from the daily light dose. These results open new perspectives for the biotechnological production of ovothiols, which are receiving a great interest for their biological activities in human model systems.A.M. and A.S. were supported by SZN Ph.D fellowships via the Open University.Peer reviewedMultidisciplinary Digital Publishing InstituteOpen UniversityConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/229186reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3390/md18090477Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2291862026-05-22T06:33:51Z
dc.title.none.fl_str_mv Insights into the light response of Skeletonema marinoi: Involvement of ovothiol
title Insights into the light response of Skeletonema marinoi: Involvement of ovothiol
spellingShingle Insights into the light response of Skeletonema marinoi: Involvement of ovothiol
Milito, Alfonsina
Algae
Ovothiol
Antioxidants
Diatoms
Light
Nitric oxide
title_short Insights into the light response of Skeletonema marinoi: Involvement of ovothiol
title_full Insights into the light response of Skeletonema marinoi: Involvement of ovothiol
title_fullStr Insights into the light response of Skeletonema marinoi: Involvement of ovothiol
title_full_unstemmed Insights into the light response of Skeletonema marinoi: Involvement of ovothiol
title_sort Insights into the light response of Skeletonema marinoi: Involvement of ovothiol
dc.creator.none.fl_str_mv Milito, Alfonsina
Orefice, Ida
Smerilli, Arianna
Castellano, Immacolata
Napolitano, Alessandra
Brunet, Christophe
Palumbo, Anna
author Milito, Alfonsina
author_facet Milito, Alfonsina
Orefice, Ida
Smerilli, Arianna
Castellano, Immacolata
Napolitano, Alessandra
Brunet, Christophe
Palumbo, Anna
author_role author
author2 Orefice, Ida
Smerilli, Arianna
Castellano, Immacolata
Napolitano, Alessandra
Brunet, Christophe
Palumbo, Anna
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Open University
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Algae
Ovothiol
Antioxidants
Diatoms
Light
Nitric oxide
topic Algae
Ovothiol
Antioxidants
Diatoms
Light
Nitric oxide
description This article belongs to the Special Issue Sulfur-Containing Marine Bioactives.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/229186
url http://hdl.handle.net/10261/229186
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/md18090477
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869416672975126528
score 15,812429

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