Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1

Background: Rhodococcus jostii RHA1 and other actinobacteria accumulate triglycerides (TAG) under nutrient starvation. This property has an important biotechnological potential in the production of sustainable oils. Results: To gain insight into the metabolic pathways involved in TAG accumulation, w...

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Autores: Juárez Giménez, Antonio, Villa, Juan A., Lanza, Val F., Lázaro, Beatriz, Cruz, Fernando de la, Alvarez, Héctor M., Moncalián, Gabriel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/123371
Acceso en línea:https://hdl.handle.net/2445/123371
Access Level:acceso abierto
Palabra clave:Bacteris anaerobis
Triglicèrids
Anaerobic bacteria
Triglycerides
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spelling Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1Juárez Giménez, AntonioVilla, Juan A.Lanza, Val F.Lázaro, BeatrizCruz, Fernando de laAlvarez, Héctor M.Moncalián, GabrielBacteris anaerobisTriglicèridsAnaerobic bacteriaTriglyceridesBackground: Rhodococcus jostii RHA1 and other actinobacteria accumulate triglycerides (TAG) under nutrient starvation. This property has an important biotechnological potential in the production of sustainable oils. Results: To gain insight into the metabolic pathways involved in TAG accumulation, we analysed the transcriptome of R jostii RHA1 under nutrient-limiting conditions. We correlate these physiological conditions with significant changes in cell physiology. The main consequence was a global switch from catabolic to anabolic pathways. Interestingly, the Entner-Doudoroff (ED) pathway was upregulated in detriment of the glycolysis or pentose phosphate pathways. ED induction was independent of the carbon source (either gluconate or glucose). Some of the diacylglycerol acyltransferase genes involved in the last step of the Kennedy pathway were also upregulated. A common feature of the promoter region of most upregulated genes was the presence of a consensus binding sequence for the cAMPdependent CRP regulator. Conclusion: This is the first experimental observation of an ED shift under nutrient starvation conditions. Knowledge of this switch could help in the design of metabolomic approaches to optimize carbon derivation for single cell oil production.BioMed Central2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/123371Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1186/s12934-017-0651-7Microbial Cell Factories, 2017, vol. 16, num. 35https://doi.org/10.1186/s12934-017-0651-7(c) BioMed Central, 2017info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1233712026-05-27T06:46:51Z
dc.title.none.fl_str_mv Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
spellingShingle Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
Juárez Giménez, Antonio
Bacteris anaerobis
Triglicèrids
Anaerobic bacteria
Triglycerides
title_short Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title_full Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title_fullStr Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title_full_unstemmed Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title_sort Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
dc.creator.none.fl_str_mv Juárez Giménez, Antonio
Villa, Juan A.
Lanza, Val F.
Lázaro, Beatriz
Cruz, Fernando de la
Alvarez, Héctor M.
Moncalián, Gabriel
author Juárez Giménez, Antonio
author_facet Juárez Giménez, Antonio
Villa, Juan A.
Lanza, Val F.
Lázaro, Beatriz
Cruz, Fernando de la
Alvarez, Héctor M.
Moncalián, Gabriel
author_role author
author2 Villa, Juan A.
Lanza, Val F.
Lázaro, Beatriz
Cruz, Fernando de la
Alvarez, Héctor M.
Moncalián, Gabriel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Bacteris anaerobis
Triglicèrids
Anaerobic bacteria
Triglycerides
topic Bacteris anaerobis
Triglicèrids
Anaerobic bacteria
Triglycerides
description Background: Rhodococcus jostii RHA1 and other actinobacteria accumulate triglycerides (TAG) under nutrient starvation. This property has an important biotechnological potential in the production of sustainable oils. Results: To gain insight into the metabolic pathways involved in TAG accumulation, we analysed the transcriptome of R jostii RHA1 under nutrient-limiting conditions. We correlate these physiological conditions with significant changes in cell physiology. The main consequence was a global switch from catabolic to anabolic pathways. Interestingly, the Entner-Doudoroff (ED) pathway was upregulated in detriment of the glycolysis or pentose phosphate pathways. ED induction was independent of the carbon source (either gluconate or glucose). Some of the diacylglycerol acyltransferase genes involved in the last step of the Kennedy pathway were also upregulated. A common feature of the promoter region of most upregulated genes was the presence of a consensus binding sequence for the cAMPdependent CRP regulator. Conclusion: This is the first experimental observation of an ED shift under nutrient starvation conditions. Knowledge of this switch could help in the design of metabolomic approaches to optimize carbon derivation for single cell oil production.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/123371
url https://hdl.handle.net/2445/123371
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1186/s12934-017-0651-7
Microbial Cell Factories, 2017, vol. 16, num. 35
https://doi.org/10.1186/s12934-017-0651-7
dc.rights.none.fl_str_mv (c) BioMed Central, 2017
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) BioMed Central, 2017
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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