Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model

Background/Aims: Alternatively activated macrophages (AAM) have regenerative and anti-inflammatory characteristics. Here, we sought to evaluate whether AAM cell therapy reduces renal inflammation and fibrosis in the unilateral ureteral obstruction (UUO) mice model. Methods: We stabilized macrophages...

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Autores: Guiteras, Roser, Sola Martínez, Anna, Flaquer, Maria, Hotter Corripio, Georgina, Torras Ambròs, Joan, Grinyó Boira, Josep M., Cruzado, Josep Ma.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/125841
Acceso en línea:https://hdl.handle.net/2445/125841
Access Level:acceso abierto
Palabra clave:Malalties cròniques
Nefrologia
Malalties del ronyó
Inflamació
Chronic diseases
Nephrology
Kidney diseases
Inflammation
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spelling Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice modelGuiteras, RoserSola Martínez, AnnaFlaquer, MariaHotter Corripio, GeorginaTorras Ambròs, JoanGrinyó Boira, Josep M.Cruzado, Josep Ma.Malalties cròniquesNefrologiaMalalties del ronyóInflamacióChronic diseasesNephrologyKidney diseasesInflammationBackground/Aims: Alternatively activated macrophages (AAM) have regenerative and anti-inflammatory characteristics. Here, we sought to evaluate whether AAM cell therapy reduces renal inflammation and fibrosis in the unilateral ureteral obstruction (UUO) mice model. Methods: We stabilized macrophages by adenoviral vector NGAL (Neutrophil gelatinase-associated lipocalin-2) and infused them into UUO mice. To ascertain whether macrophages were capable of reaching the obstructed kidney, macrophages were stained and detected by in vivo cell tracking. Results: We demonstrated that some infused macrophages reached the obstructed kidney and that infusion of macrophages overexpressing NGAL was associated with reduced kidney interstitial fibrosis and inflammation. This therapeutic effect was mainly associated with the phenotype and function preservation of the transferred macrophages isolated from the obstructed kidney Conclusions: Macrophage plasticity is a major hurdle for achieving macrophage therapy success in chronic nephropathies and could be overcome by transferring lipocalin-2.Karger2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/125841Articles publicats en revistes (Ciències Clíniques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1159/000479835Cellular Physiology and Biochemistry, 2017, vol. 42, num. 5, p. 1945-1960https://doi.org/10.1159/000479835cc-by-nc (c) Karger, 2017http://creativecommons.org/licenses/by-nc/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1258412026-05-27T06:46:51Z
dc.title.none.fl_str_mv Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model
title Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model
spellingShingle Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model
Guiteras, Roser
Malalties cròniques
Nefrologia
Malalties del ronyó
Inflamació
Chronic diseases
Nephrology
Kidney diseases
Inflammation
title_short Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model
title_full Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model
title_fullStr Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model
title_full_unstemmed Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model
title_sort Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model
dc.creator.none.fl_str_mv Guiteras, Roser
Sola Martínez, Anna
Flaquer, Maria
Hotter Corripio, Georgina
Torras Ambròs, Joan
Grinyó Boira, Josep M.
Cruzado, Josep Ma.
author Guiteras, Roser
author_facet Guiteras, Roser
Sola Martínez, Anna
Flaquer, Maria
Hotter Corripio, Georgina
Torras Ambròs, Joan
Grinyó Boira, Josep M.
Cruzado, Josep Ma.
author_role author
author2 Sola Martínez, Anna
Flaquer, Maria
Hotter Corripio, Georgina
Torras Ambròs, Joan
Grinyó Boira, Josep M.
Cruzado, Josep Ma.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Malalties cròniques
Nefrologia
Malalties del ronyó
Inflamació
Chronic diseases
Nephrology
Kidney diseases
Inflammation
topic Malalties cròniques
Nefrologia
Malalties del ronyó
Inflamació
Chronic diseases
Nephrology
Kidney diseases
Inflammation
description Background/Aims: Alternatively activated macrophages (AAM) have regenerative and anti-inflammatory characteristics. Here, we sought to evaluate whether AAM cell therapy reduces renal inflammation and fibrosis in the unilateral ureteral obstruction (UUO) mice model. Methods: We stabilized macrophages by adenoviral vector NGAL (Neutrophil gelatinase-associated lipocalin-2) and infused them into UUO mice. To ascertain whether macrophages were capable of reaching the obstructed kidney, macrophages were stained and detected by in vivo cell tracking. Results: We demonstrated that some infused macrophages reached the obstructed kidney and that infusion of macrophages overexpressing NGAL was associated with reduced kidney interstitial fibrosis and inflammation. This therapeutic effect was mainly associated with the phenotype and function preservation of the transferred macrophages isolated from the obstructed kidney Conclusions: Macrophage plasticity is a major hurdle for achieving macrophage therapy success in chronic nephropathies and could be overcome by transferring lipocalin-2.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/125841
url https://hdl.handle.net/2445/125841
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1159/000479835
Cellular Physiology and Biochemistry, 2017, vol. 42, num. 5, p. 1945-1960
https://doi.org/10.1159/000479835
dc.rights.none.fl_str_mv cc-by-nc (c) Karger, 2017
http://creativecommons.org/licenses/by-nc/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc (c) Karger, 2017
http://creativecommons.org/licenses/by-nc/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Clíniques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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