Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni.

Campylobacter, a major cause of food-borne gastroenteritis worldwide, colonize the gastrointestinal tract of a wide range of animals, being birds the main reservoir. The mechanisms involved in the interaction of Campylobacter with the different hosts are poorly understood. The cytolethal distending...

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Detalles Bibliográficos
Autores: Guirado, Pedro, Iglesias-Torrens, Yaidelis, Miró, Elisenda, Navarro, Ferran, Stephan-Otto Attolini, Camille, Balsalobre Parra, Carlos, Madrid Xufré, Cristina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/191801
Acceso en línea:https://hdl.handle.net/2445/191801
Access Level:acceso abierto
Palabra clave:Reacció en cadena de la polimerasa
Bacils
Polymerase chain reaction
Bacillus (Bacteria)
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spelling Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni.Guirado, PedroIglesias-Torrens, YaidelisMiró, ElisendaNavarro, FerranStephan-Otto Attolini, CamilleBalsalobre Parra, CarlosMadrid Xufré, CristinaReacció en cadena de la polimerasaBacilsPolymerase chain reactionBacillus (Bacteria)Campylobacter, a major cause of food-borne gastroenteritis worldwide, colonize the gastrointestinal tract of a wide range of animals, being birds the main reservoir. The mechanisms involved in the interaction of Campylobacter with the different hosts are poorly understood. The cytolethal distending toxin, encoded in the cdtABC operon, is considered a pivotal virulence factor during human infection. Differences in the prevalence of cdtABC genes in Campylobacter isolates from three distinct origins (wild birds, broiler chickens and humans) prompted us to further characterize their allelic variability. The sequence of cdtABC is highly conserved among broiler and human isolates. A high diversity of cdtABC alleles was found among wild bird isolates, including several alleles that do not produce any functional CDT. These results suggest that specific variants of the cdtABC operon might define the host range of specific Campylobacter jejuni isolates. Moreover, our data indicate that PCR methodology is inaccurate to characterize the prevalence of the cdt genes, since negative PCR detection can be the result of divergences in the sequence used for primer design rather than indicating the absence of a specific gene.Wiley2022202320222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12 p.application/pdfhttps://hdl.handle.net/2445/191801Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1111/zph.12994Zoonoses and Public Health, 2022, vol. 69, num. 8, p. 966-977https://doi.org/10.1111/zph.12994cc by-nc-nd (c) Guirado, Pedro et al., 2022http://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1918012026-05-29T05:05:01Z
dc.title.none.fl_str_mv Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni.
title Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni.
spellingShingle Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni.
Guirado, Pedro
Reacció en cadena de la polimerasa
Bacils
Polymerase chain reaction
Bacillus (Bacteria)
title_short Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni.
title_full Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni.
title_fullStr Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni.
title_full_unstemmed Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni.
title_sort Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni.
dc.creator.none.fl_str_mv Guirado, Pedro
Iglesias-Torrens, Yaidelis
Miró, Elisenda
Navarro, Ferran
Stephan-Otto Attolini, Camille
Balsalobre Parra, Carlos
Madrid Xufré, Cristina
author Guirado, Pedro
author_facet Guirado, Pedro
Iglesias-Torrens, Yaidelis
Miró, Elisenda
Navarro, Ferran
Stephan-Otto Attolini, Camille
Balsalobre Parra, Carlos
Madrid Xufré, Cristina
author_role author
author2 Iglesias-Torrens, Yaidelis
Miró, Elisenda
Navarro, Ferran
Stephan-Otto Attolini, Camille
Balsalobre Parra, Carlos
Madrid Xufré, Cristina
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Reacció en cadena de la polimerasa
Bacils
Polymerase chain reaction
Bacillus (Bacteria)
topic Reacció en cadena de la polimerasa
Bacils
Polymerase chain reaction
Bacillus (Bacteria)
description Campylobacter, a major cause of food-borne gastroenteritis worldwide, colonize the gastrointestinal tract of a wide range of animals, being birds the main reservoir. The mechanisms involved in the interaction of Campylobacter with the different hosts are poorly understood. The cytolethal distending toxin, encoded in the cdtABC operon, is considered a pivotal virulence factor during human infection. Differences in the prevalence of cdtABC genes in Campylobacter isolates from three distinct origins (wild birds, broiler chickens and humans) prompted us to further characterize their allelic variability. The sequence of cdtABC is highly conserved among broiler and human isolates. A high diversity of cdtABC alleles was found among wild bird isolates, including several alleles that do not produce any functional CDT. These results suggest that specific variants of the cdtABC operon might define the host range of specific Campylobacter jejuni isolates. Moreover, our data indicate that PCR methodology is inaccurate to characterize the prevalence of the cdt genes, since negative PCR detection can be the result of divergences in the sequence used for primer design rather than indicating the absence of a specific gene.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/191801
url https://hdl.handle.net/2445/191801
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1111/zph.12994
Zoonoses and Public Health, 2022, vol. 69, num. 8, p. 966-977
https://doi.org/10.1111/zph.12994
dc.rights.none.fl_str_mv cc by-nc-nd (c) Guirado, Pedro et al., 2022
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by-nc-nd (c) Guirado, Pedro et al., 2022
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12 p.
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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