Tetracycline and multidrug resistance in the oral microbiota: differences between healthy subjects and patients with periodontitis in Spain

Introduction: Antibiotic resistance is widely found even among bacterial populations not having been exposed to selective pressure by antibiotics, such as tetracycline. In this study we analyzed the tetracycline-resistant subgingival microbiota of healthy subjects and of patients with periodontitis,...

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Detalles Bibliográficos
Autores: Arredondo, Alexandre, Blanc, Vanessa, Mor Reinoso, Carolina, Nart Molina, José, León, Rubén
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12328/2599
Acceso en línea:http://hdl.handle.net/20.500.12328/2599
https://doi.org/10.1080/20002297.2020.1847431
Access Level:acceso abierto
Palabra clave:Resistència als antibiòtics
Antibiòtics
Tetraciclina
Periodontitis
Microbiota subgingival
Resistència a múltiples fàrmacs
Resistencia a los antibióticos
Antibióticos
Resistencia a múltiples fármacos
Antibiotic resistance
Antibiotics
Tetracycline
Subgingival microbiota
Resistance to multiple drugs
616.3
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spelling Tetracycline and multidrug resistance in the oral microbiota: differences between healthy subjects and patients with periodontitis in SpainArredondo, AlexandreBlanc, VanessaMor Reinoso, CarolinaNart Molina, JoséLeón, RubénResistència als antibiòticsAntibiòticsTetraciclinaPeriodontitisMicrobiota subgingivalResistència a múltiples fàrmacsResistencia a los antibióticosAntibióticosTetraciclinaPeriodontitisMicrobiota subgingivalResistencia a múltiples fármacosAntibiotic resistanceAntibioticsTetracyclinePeriodontitisSubgingival microbiotaResistance to multiple drugs616.3Introduction: Antibiotic resistance is widely found even among bacterial populations not having been exposed to selective pressure by antibiotics, such as tetracycline. In this study we analyzed the tetracycline-resistant subgingival microbiota of healthy subjects and of patients with periodontitis, comparing the prevalence of tet genes and their multidrug resistance profiles. Methods: Samples from 259 volunteers were analyzed, obtaining 813 tetracycline-resistant isolates. The prevalence of 12 antibiotic resistance genes was assessed, and multidrug profiles were built. Each isolate was identified by 16S rRNA sequencing. Differences in qualitative data and quantitative data were evaluated using the chi-square test and the Mann-Whitney-U test, respectively. Results: tet(M) was the most frequently detected tet gene (52.03%). We observed significant differences between the prevalence of tet(M), tet(W), tet(O), tet(32) and tet(L) in both populations studied. Multidrug resistance was largely observed, with resistance to kanamycin being the most detected (83.64%). There were significant differences between the populations in the prevalence of kanamycin, chloramphenicol, and cefotaxime resistance. Resistant isolates showed significantly different prevalence between the two studied groups. Conclusion: The high prevalence of multidrug resistance and tetracycline resistance genes found in the subgingival microbiota, highlights the importance of performing wider and more in-depth analysis of antibiotic resistance in the oral microbiota.info:eu-repo/semantics/publishedVersionTaylor & Francis2020info:eu-repo/semantics/article10http://hdl.handle.net/20.500.12328/2599https://doi.org/10.1080/20002297.2020.1847431reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésJournal of Oral Microbiology13;1© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.https://creativecommons.org/licenses/by/3.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12328/25992026-05-29T05:05:01Z
dc.title.none.fl_str_mv Tetracycline and multidrug resistance in the oral microbiota: differences between healthy subjects and patients with periodontitis in Spain
title Tetracycline and multidrug resistance in the oral microbiota: differences between healthy subjects and patients with periodontitis in Spain
spellingShingle Tetracycline and multidrug resistance in the oral microbiota: differences between healthy subjects and patients with periodontitis in Spain
Arredondo, Alexandre
Resistència als antibiòtics
Antibiòtics
Tetraciclina
Periodontitis
Microbiota subgingival
Resistència a múltiples fàrmacs
Resistencia a los antibióticos
Antibióticos
Tetraciclina
Periodontitis
Microbiota subgingival
Resistencia a múltiples fármacos
Antibiotic resistance
Antibiotics
Tetracycline
Periodontitis
Subgingival microbiota
Resistance to multiple drugs
616.3
title_short Tetracycline and multidrug resistance in the oral microbiota: differences between healthy subjects and patients with periodontitis in Spain
title_full Tetracycline and multidrug resistance in the oral microbiota: differences between healthy subjects and patients with periodontitis in Spain
title_fullStr Tetracycline and multidrug resistance in the oral microbiota: differences between healthy subjects and patients with periodontitis in Spain
title_full_unstemmed Tetracycline and multidrug resistance in the oral microbiota: differences between healthy subjects and patients with periodontitis in Spain
title_sort Tetracycline and multidrug resistance in the oral microbiota: differences between healthy subjects and patients with periodontitis in Spain
dc.creator.none.fl_str_mv Arredondo, Alexandre
Blanc, Vanessa
Mor Reinoso, Carolina
Nart Molina, José
León, Rubén
author Arredondo, Alexandre
author_facet Arredondo, Alexandre
Blanc, Vanessa
Mor Reinoso, Carolina
Nart Molina, José
León, Rubén
author_role author
author2 Blanc, Vanessa
Mor Reinoso, Carolina
Nart Molina, José
León, Rubén
author2_role author
author
author
author
dc.subject.none.fl_str_mv Resistència als antibiòtics
Antibiòtics
Tetraciclina
Periodontitis
Microbiota subgingival
Resistència a múltiples fàrmacs
Resistencia a los antibióticos
Antibióticos
Tetraciclina
Periodontitis
Microbiota subgingival
Resistencia a múltiples fármacos
Antibiotic resistance
Antibiotics
Tetracycline
Periodontitis
Subgingival microbiota
Resistance to multiple drugs
616.3
topic Resistència als antibiòtics
Antibiòtics
Tetraciclina
Periodontitis
Microbiota subgingival
Resistència a múltiples fàrmacs
Resistencia a los antibióticos
Antibióticos
Tetraciclina
Periodontitis
Microbiota subgingival
Resistencia a múltiples fármacos
Antibiotic resistance
Antibiotics
Tetracycline
Periodontitis
Subgingival microbiota
Resistance to multiple drugs
616.3
description Introduction: Antibiotic resistance is widely found even among bacterial populations not having been exposed to selective pressure by antibiotics, such as tetracycline. In this study we analyzed the tetracycline-resistant subgingival microbiota of healthy subjects and of patients with periodontitis, comparing the prevalence of tet genes and their multidrug resistance profiles. Methods: Samples from 259 volunteers were analyzed, obtaining 813 tetracycline-resistant isolates. The prevalence of 12 antibiotic resistance genes was assessed, and multidrug profiles were built. Each isolate was identified by 16S rRNA sequencing. Differences in qualitative data and quantitative data were evaluated using the chi-square test and the Mann-Whitney-U test, respectively. Results: tet(M) was the most frequently detected tet gene (52.03%). We observed significant differences between the prevalence of tet(M), tet(W), tet(O), tet(32) and tet(L) in both populations studied. Multidrug resistance was largely observed, with resistance to kanamycin being the most detected (83.64%). There were significant differences between the populations in the prevalence of kanamycin, chloramphenicol, and cefotaxime resistance. Resistant isolates showed significantly different prevalence between the two studied groups. Conclusion: The high prevalence of multidrug resistance and tetracycline resistance genes found in the subgingival microbiota, highlights the importance of performing wider and more in-depth analysis of antibiotic resistance in the oral microbiota.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12328/2599
https://doi.org/10.1080/20002297.2020.1847431
url http://hdl.handle.net/20.500.12328/2599
https://doi.org/10.1080/20002297.2020.1847431
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Oral Microbiology
13;1
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/3.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/3.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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