Mycobiome Profiles in Breast Milk from Healthy Women Depend on Mode of Delivery, Geographic Location, and Interaction with Bacteria

Recent studies report the presence of fungal species in breast milk of healthy mothers, suggesting a potential role in infant mycobiome development. In the present work, we aimed to determine whether the healthy human breast milk mycobiota is influenced by geographical location and mode of delivery,...

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Authors: Boix-Amorós, Alba, Puente-Sánchez, Fernando, Du Toit, Elloise, Linderborg, Kaisa M., Zhang, Yumei, Yang, Baoru, Salminen, Seppo, Isolauri, Erika, Tamames, Javier, Mira, Alex, Collado, María Carmen
Format: article
Status:Versión aceptada para publicación
Publication Date:2019
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/184050
Online Access:http://hdl.handle.net/10261/184050
Access Level:Open access
Keyword:Multiple sequence alignment
Candida albicans
Fungal communities
Microbiota
Oral Candida
Lactation
Breast milk
Illumina sequencing
id ES_aebfd08f989a608da89e8fdf3bce1a79
oai_identifier_str oai:digital.csic.es:10261/184050
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Mycobiome Profiles in Breast Milk from Healthy Women Depend on Mode of Delivery, Geographic Location, and Interaction with Bacteria
title Mycobiome Profiles in Breast Milk from Healthy Women Depend on Mode of Delivery, Geographic Location, and Interaction with Bacteria
spellingShingle Mycobiome Profiles in Breast Milk from Healthy Women Depend on Mode of Delivery, Geographic Location, and Interaction with Bacteria
Boix-Amorós, Alba
Multiple sequence alignment
Candida albicans
Fungal communities
Microbiota
Oral Candida
Lactation
Breast milk
Illumina sequencing
title_short Mycobiome Profiles in Breast Milk from Healthy Women Depend on Mode of Delivery, Geographic Location, and Interaction with Bacteria
title_full Mycobiome Profiles in Breast Milk from Healthy Women Depend on Mode of Delivery, Geographic Location, and Interaction with Bacteria
title_fullStr Mycobiome Profiles in Breast Milk from Healthy Women Depend on Mode of Delivery, Geographic Location, and Interaction with Bacteria
title_full_unstemmed Mycobiome Profiles in Breast Milk from Healthy Women Depend on Mode of Delivery, Geographic Location, and Interaction with Bacteria
title_sort Mycobiome Profiles in Breast Milk from Healthy Women Depend on Mode of Delivery, Geographic Location, and Interaction with Bacteria
dc.creator.none.fl_str_mv Boix-Amorós, Alba
Puente-Sánchez, Fernando
Du Toit, Elloise
Linderborg, Kaisa M.
Zhang, Yumei
Yang, Baoru
Salminen, Seppo
Isolauri, Erika
Tamames, Javier
Mira, Alex
Collado, María Carmen
author Boix-Amorós, Alba
author_facet Boix-Amorós, Alba
Puente-Sánchez, Fernando
Du Toit, Elloise
Linderborg, Kaisa M.
Zhang, Yumei
Yang, Baoru
Salminen, Seppo
Isolauri, Erika
Tamames, Javier
Mira, Alex
Collado, María Carmen
author_role author
author2 Puente-Sánchez, Fernando
Du Toit, Elloise
Linderborg, Kaisa M.
Zhang, Yumei
Yang, Baoru
Salminen, Seppo
Isolauri, Erika
Tamames, Javier
Mira, Alex
Collado, María Carmen
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Research Council
Ministerio de Economía y Competitividad (España)
Beijing science and Technology Development
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Multiple sequence alignment
Candida albicans
Fungal communities
Microbiota
Oral Candida
Lactation
Breast milk
Illumina sequencing
topic Multiple sequence alignment
Candida albicans
Fungal communities
Microbiota
Oral Candida
Lactation
Breast milk
Illumina sequencing
description Recent studies report the presence of fungal species in breast milk of healthy mothers, suggesting a potential role in infant mycobiome development. In the present work, we aimed to determine whether the healthy human breast milk mycobiota is influenced by geographical location and mode of delivery, as well as to investigate its interaction with bacterial profiles in the same samples. A total of 80 mature breast milk samples from 4 different countries were analyzed by Illumina sequencing of the internal transcribed spacer 1 (ITS1) region, joining the 18S and 5.8S regions of the fungal rRNA region. Basidiomycota and Ascomycota were found to be the dominant phyla, with Malassezia and Davidiella being the most prevalent genera across countries. A core formed by Malassezia, Davidiella, Sistotrema, and Penicillium was shared in the milk samples from the different origins, although specific shifts in mycobiome composition were associated with geographic location and delivery mode. The presence of fungi in the breast milk samples was further confirmed by culture and isolate characterization, and fungal loads were estimated by quantitative PCR (qPCR) targeting the fungal ITS1 region. Cooccurrence network analysis of bacteria and fungi showed complex interactions that were influenced by geographical location, mode of delivery, maternal age, and pregestational body mass index. The presence of a breast milk mycobiome was confirmed in all samples analyzed, regardless of the geographic origin. IMPORTANCE During recent years, human breast milk has been documented as a potential source of bacteria for the newborn. Recently, we have reported the presence of fungi in breast milk from healthy mothers. It is well known that environmental and perinatal factors can affect milk bacteria; however, the impact on milk fungi is still unknown. The current report describes fungal communities (mycobiota) in breast milk samples across different geographic locations and the influence of the mode of delivery. We also provide novel insights on bacterium-fungus interactions, taking into account environmental and perinatal factors. We identified a core of four genera shared across locations, consisting of Malassezia, Davidiella, Sistotrema, and Penicillium, which have been reported to be present in the infant gut. Our data confirm the presence of fungi in breast milk across continents and support the potential role of breast milk in the initial seeding of fungal species in the infant gut.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/184050
url http://hdl.handle.net/10261/184050
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/FP7/639226
https://doi.org/10.1128/AEM.02994-18
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Mycobiome Profiles in Breast Milk from Healthy Women Depend on Mode of Delivery, Geographic Location, and Interaction with BacteriaBoix-Amorós, AlbaPuente-Sánchez, FernandoDu Toit, ElloiseLinderborg, Kaisa M.Zhang, YumeiYang, BaoruSalminen, SeppoIsolauri, ErikaTamames, JavierMira, AlexCollado, María CarmenMultiple sequence alignmentCandida albicansFungal communitiesMicrobiotaOral CandidaLactationBreast milkIllumina sequencingRecent studies report the presence of fungal species in breast milk of healthy mothers, suggesting a potential role in infant mycobiome development. In the present work, we aimed to determine whether the healthy human breast milk mycobiota is influenced by geographical location and mode of delivery, as well as to investigate its interaction with bacterial profiles in the same samples. A total of 80 mature breast milk samples from 4 different countries were analyzed by Illumina sequencing of the internal transcribed spacer 1 (ITS1) region, joining the 18S and 5.8S regions of the fungal rRNA region. Basidiomycota and Ascomycota were found to be the dominant phyla, with Malassezia and Davidiella being the most prevalent genera across countries. A core formed by Malassezia, Davidiella, Sistotrema, and Penicillium was shared in the milk samples from the different origins, although specific shifts in mycobiome composition were associated with geographic location and delivery mode. The presence of fungi in the breast milk samples was further confirmed by culture and isolate characterization, and fungal loads were estimated by quantitative PCR (qPCR) targeting the fungal ITS1 region. Cooccurrence network analysis of bacteria and fungi showed complex interactions that were influenced by geographical location, mode of delivery, maternal age, and pregestational body mass index. The presence of a breast milk mycobiome was confirmed in all samples analyzed, regardless of the geographic origin. IMPORTANCE During recent years, human breast milk has been documented as a potential source of bacteria for the newborn. Recently, we have reported the presence of fungi in breast milk from healthy mothers. It is well known that environmental and perinatal factors can affect milk bacteria; however, the impact on milk fungi is still unknown. The current report describes fungal communities (mycobiota) in breast milk samples across different geographic locations and the influence of the mode of delivery. We also provide novel insights on bacterium-fungus interactions, taking into account environmental and perinatal factors. We identified a core of four genera shared across locations, consisting of Malassezia, Davidiella, Sistotrema, and Penicillium, which have been reported to be present in the infant gut. Our data confirm the presence of fungi in breast milk across continents and support the potential role of breast milk in the initial seeding of fungal species in the infant gut.A.B.-A. and M.C.C. acknowledge the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (ERC Starting Grant project no. 639226). F.P.-S. was supported by Ministerio de Economía y Competitividad (MINECO) grant no. CTM2016-80095-C2-1-R (NOVAMAR). The Chinese group acknowledges support from Key Projects of Beijing Science and Technology (D141100004814002) and the Natural Scientific Foundation of Beijing (Z140001).Peer reviewedAmerican Society for MicrobiologyEuropean Research CouncilMinisterio de Economía y Competitividad (España)Beijing science and Technology DevelopmentConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/184050reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/FP7/639226https://doi.org/10.1128/AEM.02994-18Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1840502026-05-22T06:33:51Z
score 15,811543

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