Epithelial cell cluster size affects force distribution in response to EGF-induced collective contractility

Several factors present in the extracellular environment regulate epithelial cell adhesion and dynamics. Among them, growth factors such as EGF, upon binding to their receptors at the cell surface, get internalized and directly activate the acto-myosin machinery. In this study we present the effects...

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Autores: Zambarda, Chiara, Pérez González, Carlos, Schoenit, Andreas, Veits, Nisha, Schimmer, Clara, Jung, Raimund, Ollech, Dirk, Christian, Joel, Roca-Cusachs Soulere, Pere, Trepat Guixer, Xavier, Cavalcanti Adam, Elisabetta Ada
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/191215
Acesso em linha:https://hdl.handle.net/2445/191215
Access Level:acceso abierto
Palavra-chave:Cèl·lules
Factor de creixement epidèrmic
Proteïnes
Cèl·lules canceroses
Cèl·lules epitelials
Interacció cel·lular
Cells
Epidermal growth factor
Proteins
Cancer cells
Epithelial cells
Cell interaction
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spelling Epithelial cell cluster size affects force distribution in response to EGF-induced collective contractilityZambarda, ChiaraPérez González, CarlosSchoenit, AndreasVeits, NishaSchimmer, ClaraJung, RaimundOllech, DirkChristian, JoelRoca-Cusachs Soulere, PereTrepat Guixer, XavierCavalcanti Adam, Elisabetta AdaCèl·lulesFactor de creixement epidèrmicProteïnesCèl·lules cancerosesCèl·lules epitelialsInteracció cel·lularCellsEpidermal growth factorProteinsCancer cellsEpithelial cellsCell interactionSeveral factors present in the extracellular environment regulate epithelial cell adhesion and dynamics. Among them, growth factors such as EGF, upon binding to their receptors at the cell surface, get internalized and directly activate the acto-myosin machinery. In this study we present the effects of EGF on the contractility of epithelial cancer cell colonies in confined geometry of different sizes. We show that the extent to which EGF triggers contractility scales with the cluster size and thus the number of cells. Moreover, the collective contractility results in a radial distribution of traction forces, which are dependent on integrin β1 peripheral adhesions and transmitted to neighboring cells through adherens junctions. Taken together, EGF-induced contractility acts on the mechanical crosstalk and linkage between the cell-cell and cell-matrix compartments, regulating collective responses.Elsevier2022202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12 p.application/pdfhttps://hdl.handle.net/2445/191215Articles publicats en revistes (Biomedicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1016/j.ejcb.2022.151274European Journal of Cell Biology, 2022, vol. 101, num. 4, p. 151274https://doi.org/10.1016/j.ejcb.2022.151274cc-by-nc-nd (c) Zambarda, Chiara et al., 2022https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1912152026-05-29T05:05:01Z
dc.title.none.fl_str_mv Epithelial cell cluster size affects force distribution in response to EGF-induced collective contractility
title Epithelial cell cluster size affects force distribution in response to EGF-induced collective contractility
spellingShingle Epithelial cell cluster size affects force distribution in response to EGF-induced collective contractility
Zambarda, Chiara
Cèl·lules
Factor de creixement epidèrmic
Proteïnes
Cèl·lules canceroses
Cèl·lules epitelials
Interacció cel·lular
Cells
Epidermal growth factor
Proteins
Cancer cells
Epithelial cells
Cell interaction
title_short Epithelial cell cluster size affects force distribution in response to EGF-induced collective contractility
title_full Epithelial cell cluster size affects force distribution in response to EGF-induced collective contractility
title_fullStr Epithelial cell cluster size affects force distribution in response to EGF-induced collective contractility
title_full_unstemmed Epithelial cell cluster size affects force distribution in response to EGF-induced collective contractility
title_sort Epithelial cell cluster size affects force distribution in response to EGF-induced collective contractility
dc.creator.none.fl_str_mv Zambarda, Chiara
Pérez González, Carlos
Schoenit, Andreas
Veits, Nisha
Schimmer, Clara
Jung, Raimund
Ollech, Dirk
Christian, Joel
Roca-Cusachs Soulere, Pere
Trepat Guixer, Xavier
Cavalcanti Adam, Elisabetta Ada
author Zambarda, Chiara
author_facet Zambarda, Chiara
Pérez González, Carlos
Schoenit, Andreas
Veits, Nisha
Schimmer, Clara
Jung, Raimund
Ollech, Dirk
Christian, Joel
Roca-Cusachs Soulere, Pere
Trepat Guixer, Xavier
Cavalcanti Adam, Elisabetta Ada
author_role author
author2 Pérez González, Carlos
Schoenit, Andreas
Veits, Nisha
Schimmer, Clara
Jung, Raimund
Ollech, Dirk
Christian, Joel
Roca-Cusachs Soulere, Pere
Trepat Guixer, Xavier
Cavalcanti Adam, Elisabetta Ada
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cèl·lules
Factor de creixement epidèrmic
Proteïnes
Cèl·lules canceroses
Cèl·lules epitelials
Interacció cel·lular
Cells
Epidermal growth factor
Proteins
Cancer cells
Epithelial cells
Cell interaction
topic Cèl·lules
Factor de creixement epidèrmic
Proteïnes
Cèl·lules canceroses
Cèl·lules epitelials
Interacció cel·lular
Cells
Epidermal growth factor
Proteins
Cancer cells
Epithelial cells
Cell interaction
description Several factors present in the extracellular environment regulate epithelial cell adhesion and dynamics. Among them, growth factors such as EGF, upon binding to their receptors at the cell surface, get internalized and directly activate the acto-myosin machinery. In this study we present the effects of EGF on the contractility of epithelial cancer cell colonies in confined geometry of different sizes. We show that the extent to which EGF triggers contractility scales with the cluster size and thus the number of cells. Moreover, the collective contractility results in a radial distribution of traction forces, which are dependent on integrin β1 peripheral adhesions and transmitted to neighboring cells through adherens junctions. Taken together, EGF-induced contractility acts on the mechanical crosstalk and linkage between the cell-cell and cell-matrix compartments, regulating collective responses.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/191215
url https://hdl.handle.net/2445/191215
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.ejcb.2022.151274
European Journal of Cell Biology, 2022, vol. 101, num. 4, p. 151274
https://doi.org/10.1016/j.ejcb.2022.151274
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Zambarda, Chiara et al., 2022
https://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Zambarda, Chiara et al., 2022
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869416595266207744
score 15.81155

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