Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells
The aim of this study was to assess free fatty acids' (FAs) ex vivo anti-/proinflammatory capabilities and their influence on inflammatory gene expression and H2O2 production by human peripheral blood mononuclear cells (PBMCs). Anthropometric and clinical measurements were performed in 26 parti...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Conselleria de Salut i Consum del Govern de les Illes Balears |
| Repositorio: | Docusalut |
| Idioma: | inglés |
| OAI Identifier: | oai:docusalut.com:20.500.13003/18237 |
| Acceso en línea: | https://hdl.handle.net/20.500.13003/18237 |
| Access Level: | acceso abierto |
| Palabra clave: | Metabolic Syndrome Aged, 80 and over Oxidative Stress Aged Humans Leukocytes, Mononuclear Middle Aged Hydrogen Peroxide Male Fatty Acids, Nonesterified NF-kappa B Inflammation Mediators Tumor Necrosis Factor-alpha Female Toll-Like Receptor 2 Interleukin-6 Gene Expression Lipopolysaccharides Cyclooxygenase 2 Lipopolisacáridos Expresión Génica Interleucina-6 Mediadores de Inflamación FN-kappa B Síndrome Metabólico Femenino Factor de Necrosis Tumoral alfa Ácidos Grasos no Esterificados Masculino Peróxido de Hidrógeno Receptor Toll-Like 2 Humanos Persona de Mediana Edad Leucocitos Mononucleares Anciano Anciano de 80 o más Años Ciclooxigenasa 2 Estrés Oxidativo |
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Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear CellsSureda, AntoniMartorell, MiquelBibiloni Esteva, Maria Del MarBouzas, CristinaGallardo-Alfaro, LauraMateos, DavidCapó Fiol, XavierTur, Josep A.Pons, AntoniMetabolic SyndromeAged, 80 and overOxidative StressAgedHumansLeukocytes, MononuclearMiddle AgedHydrogen PeroxideMaleFatty Acids, NonesterifiedNF-kappa BInflammation MediatorsTumor Necrosis Factor-alphaFemaleToll-Like Receptor 2Interleukin-6Gene ExpressionLipopolysaccharidesCyclooxygenase 2Aged, 80 and overLipopolisacáridosExpresión GénicaInterleucina-6Mediadores de InflamaciónFN-kappa BSíndrome MetabólicoFemeninoFactor de Necrosis Tumoral alfaÁcidos Grasos no EsterificadosMasculinoPeróxido de HidrógenoReceptor Toll-Like 2HumanosPersona de Mediana EdadLeucocitos MononuclearesAncianoAnciano de 80 o más AñosCiclooxigenasa 2Estrés OxidativoThe aim of this study was to assess free fatty acids' (FAs) ex vivo anti-/proinflammatory capabilities and their influence on inflammatory gene expression and H2O2 production by human peripheral blood mononuclear cells (PBMCs). Anthropometric and clinical measurements were performed in 26 participants with metabolic syndrome. Isolated PBMCs were incubated ex vivo for 2 h with several free fatty acids-palmitic, oleic, α-linolenic, γ-linolenic, arachidonic and docosahexaenoic at 50 μM, and lipopolysaccharide (LPS) alone or in combination. H2O2 production and IL6, NFκB, TLR2, TNFα, and COX-2 gene expressions were determined. Palmitic, γ-linolenic, and arachidonic acids showed minor effects on inflammatory gene expression, whereas oleic, α-linolenic, and docosahexaenoic acids reduced proinflammatory gene expression in LPS-stimulated PBMCs. Arachidonic and α-linolenic acids treatment enhanced LPS-stimulated H2O2 production by PBMCs, while palmitic, oleic, γ-linolenic, and docosahexaenoic acids did not exert significant effects. Oleic, α-linolenic, and docosahexaenoic acids induced anti-inflammatory responses in PBMCs. Arachidonic and α-linolenic acids enhanced the oxidative status of LPS-stimulated PBMCs. In conclusion, PBMC ex vivo assays are useful to assess the anti-/proinflammatory and redox-modulatory effects of fatty acids or other food bioactive compounds.MDPI20202020-01-0420202020-01-04research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.13003/18237reponame:Docusalutinstname:Conselleria de Salut i Consum del Govern de les Illes BalearsInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docusalut.com:20.500.13003/182372026-06-22T12:44:07Z |
| dc.title.none.fl_str_mv |
Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells |
| title |
Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells |
| spellingShingle |
Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells Sureda, Antoni Metabolic Syndrome Aged, 80 and over Oxidative Stress Aged Humans Leukocytes, Mononuclear Middle Aged Hydrogen Peroxide Male Fatty Acids, Nonesterified NF-kappa B Inflammation Mediators Tumor Necrosis Factor-alpha Female Toll-Like Receptor 2 Interleukin-6 Gene Expression Lipopolysaccharides Cyclooxygenase 2 Aged, 80 and over Lipopolisacáridos Expresión Génica Interleucina-6 Mediadores de Inflamación FN-kappa B Síndrome Metabólico Femenino Factor de Necrosis Tumoral alfa Ácidos Grasos no Esterificados Masculino Peróxido de Hidrógeno Receptor Toll-Like 2 Humanos Persona de Mediana Edad Leucocitos Mononucleares Anciano Anciano de 80 o más Años Ciclooxigenasa 2 Estrés Oxidativo |
| title_short |
Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells |
| title_full |
Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells |
| title_fullStr |
Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells |
| title_full_unstemmed |
Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells |
| title_sort |
Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells |
| dc.creator.none.fl_str_mv |
Sureda, Antoni Martorell, Miquel Bibiloni Esteva, Maria Del Mar Bouzas, Cristina Gallardo-Alfaro, Laura Mateos, David Capó Fiol, Xavier Tur, Josep A. Pons, Antoni |
| author |
Sureda, Antoni |
| author_facet |
Sureda, Antoni Martorell, Miquel Bibiloni Esteva, Maria Del Mar Bouzas, Cristina Gallardo-Alfaro, Laura Mateos, David Capó Fiol, Xavier Tur, Josep A. Pons, Antoni |
| author_role |
author |
| author2 |
Martorell, Miquel Bibiloni Esteva, Maria Del Mar Bouzas, Cristina Gallardo-Alfaro, Laura Mateos, David Capó Fiol, Xavier Tur, Josep A. Pons, Antoni |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
|
| dc.subject.none.fl_str_mv |
Metabolic Syndrome Aged, 80 and over Oxidative Stress Aged Humans Leukocytes, Mononuclear Middle Aged Hydrogen Peroxide Male Fatty Acids, Nonesterified NF-kappa B Inflammation Mediators Tumor Necrosis Factor-alpha Female Toll-Like Receptor 2 Interleukin-6 Gene Expression Lipopolysaccharides Cyclooxygenase 2 Aged, 80 and over Lipopolisacáridos Expresión Génica Interleucina-6 Mediadores de Inflamación FN-kappa B Síndrome Metabólico Femenino Factor de Necrosis Tumoral alfa Ácidos Grasos no Esterificados Masculino Peróxido de Hidrógeno Receptor Toll-Like 2 Humanos Persona de Mediana Edad Leucocitos Mononucleares Anciano Anciano de 80 o más Años Ciclooxigenasa 2 Estrés Oxidativo |
| topic |
Metabolic Syndrome Aged, 80 and over Oxidative Stress Aged Humans Leukocytes, Mononuclear Middle Aged Hydrogen Peroxide Male Fatty Acids, Nonesterified NF-kappa B Inflammation Mediators Tumor Necrosis Factor-alpha Female Toll-Like Receptor 2 Interleukin-6 Gene Expression Lipopolysaccharides Cyclooxygenase 2 Aged, 80 and over Lipopolisacáridos Expresión Génica Interleucina-6 Mediadores de Inflamación FN-kappa B Síndrome Metabólico Femenino Factor de Necrosis Tumoral alfa Ácidos Grasos no Esterificados Masculino Peróxido de Hidrógeno Receptor Toll-Like 2 Humanos Persona de Mediana Edad Leucocitos Mononucleares Anciano Anciano de 80 o más Años Ciclooxigenasa 2 Estrés Oxidativo |
| description |
The aim of this study was to assess free fatty acids' (FAs) ex vivo anti-/proinflammatory capabilities and their influence on inflammatory gene expression and H2O2 production by human peripheral blood mononuclear cells (PBMCs). Anthropometric and clinical measurements were performed in 26 participants with metabolic syndrome. Isolated PBMCs were incubated ex vivo for 2 h with several free fatty acids-palmitic, oleic, α-linolenic, γ-linolenic, arachidonic and docosahexaenoic at 50 μM, and lipopolysaccharide (LPS) alone or in combination. H2O2 production and IL6, NFκB, TLR2, TNFα, and COX-2 gene expressions were determined. Palmitic, γ-linolenic, and arachidonic acids showed minor effects on inflammatory gene expression, whereas oleic, α-linolenic, and docosahexaenoic acids reduced proinflammatory gene expression in LPS-stimulated PBMCs. Arachidonic and α-linolenic acids treatment enhanced LPS-stimulated H2O2 production by PBMCs, while palmitic, oleic, γ-linolenic, and docosahexaenoic acids did not exert significant effects. Oleic, α-linolenic, and docosahexaenoic acids induced anti-inflammatory responses in PBMCs. Arachidonic and α-linolenic acids enhanced the oxidative status of LPS-stimulated PBMCs. In conclusion, PBMC ex vivo assays are useful to assess the anti-/proinflammatory and redox-modulatory effects of fatty acids or other food bioactive compounds. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2020-01-04 2020 2020-01-04 |
| dc.type.none.fl_str_mv |
research article http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/20.500.13003/18237 |
| url |
https://hdl.handle.net/20.500.13003/18237 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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reponame:Docusalut instname:Conselleria de Salut i Consum del Govern de les Illes Balears |
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Conselleria de Salut i Consum del Govern de les Illes Balears |
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