Genomic Validation of Endometrial Cancer Patient-Derived Xenograft Models as a Preclinical Tool

Bioinformatics; Endometrial cancer; Molecular marker

Detalhes bibliográficos
Autores: Villafranca Magdalena, Beatriz, Masferrer Ferragutcasas, Carina, Lopez Gil, Carlos, Coll De la Rubia, Eva, Rebull Santamaria, Marta, Parra, Genis, García Jiménez, Angel, Cabrera Diaz, Silvia, Colas Ortega, Eva, Gil Moreno, Antonio, Reques Llanos, Armando, Moiola, Cristian Pablo
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:11351/7849
Acesso em linha:https://hdl.handle.net/11351/7849
Access Level:acceso abierto
Palavra-chave:Endometri - Càncer
Genòmica
DISEASES::Neoplasms::Neoplasms by Site::Urogenital Neoplasms::Genital Neoplasms, Female::Uterine Neoplasms::Endometrial Neoplasms
Other subheadings::Other subheadings::Other subheadings::/genetics
ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias urogenitales::neoplasias de los genitales femeninos::neoplasias uterinas::neoplasias endometriales
Otros calificadores::Otros calificadores::Otros calificadores::/genética
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oai_identifier_str oai:recercat.cat:11351/7849
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Genomic Validation of Endometrial Cancer Patient-Derived Xenograft Models as a Preclinical Tool
title Genomic Validation of Endometrial Cancer Patient-Derived Xenograft Models as a Preclinical Tool
spellingShingle Genomic Validation of Endometrial Cancer Patient-Derived Xenograft Models as a Preclinical Tool
Villafranca Magdalena, Beatriz
Endometri - Càncer
Genòmica
DISEASES::Neoplasms::Neoplasms by Site::Urogenital Neoplasms::Genital Neoplasms, Female::Uterine Neoplasms::Endometrial Neoplasms
Other subheadings::Other subheadings::Other subheadings::/genetics
ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias urogenitales::neoplasias de los genitales femeninos::neoplasias uterinas::neoplasias endometriales
Otros calificadores::Otros calificadores::Otros calificadores::/genética
title_short Genomic Validation of Endometrial Cancer Patient-Derived Xenograft Models as a Preclinical Tool
title_full Genomic Validation of Endometrial Cancer Patient-Derived Xenograft Models as a Preclinical Tool
title_fullStr Genomic Validation of Endometrial Cancer Patient-Derived Xenograft Models as a Preclinical Tool
title_full_unstemmed Genomic Validation of Endometrial Cancer Patient-Derived Xenograft Models as a Preclinical Tool
title_sort Genomic Validation of Endometrial Cancer Patient-Derived Xenograft Models as a Preclinical Tool
dc.creator.none.fl_str_mv Villafranca Magdalena, Beatriz
Masferrer Ferragutcasas, Carina
Lopez Gil, Carlos
Coll De la Rubia, Eva
Rebull Santamaria, Marta
Parra, Genis
García Jiménez, Angel
Cabrera Diaz, Silvia
Colas Ortega, Eva
Gil Moreno, Antonio
Reques Llanos, Armando
Moiola, Cristian Pablo
author Villafranca Magdalena, Beatriz
author_facet Villafranca Magdalena, Beatriz
Masferrer Ferragutcasas, Carina
Lopez Gil, Carlos
Coll De la Rubia, Eva
Rebull Santamaria, Marta
Parra, Genis
García Jiménez, Angel
Cabrera Diaz, Silvia
Colas Ortega, Eva
Gil Moreno, Antonio
Reques Llanos, Armando
Moiola, Cristian Pablo
author_role author
author2 Masferrer Ferragutcasas, Carina
Lopez Gil, Carlos
Coll De la Rubia, Eva
Rebull Santamaria, Marta
Parra, Genis
García Jiménez, Angel
Cabrera Diaz, Silvia
Colas Ortega, Eva
Gil Moreno, Antonio
Reques Llanos, Armando
Moiola, Cristian Pablo
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Institut Català de la Salut
[Villafranca-Magdalena B, Masferrer-Ferragutcasas C, Lopez-Gil C, Rebull M, Moiola CP] Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Escola de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Coll-de la Rubia E, Colas E] Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Escola de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. Biomedical Research Center Network (CIBERONC), Madrid, Spain. [Parra G] National Center for Genomic Analysis—Genomic Regulation Center (CNAG-CRG), Scientific Park of Barcelona, Barcelona, Spain. [García A, Reques A] Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Servei de Patologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Cabrera S, Gil-Moreno A] Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Escola de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. Biomedical Research Center Network (CIBERONC), Madrid, Spain. Servei de Ginecologia Oncològica, Vall d’Hebron Hospital Universitari, Barcelona, Spain
Vall d'Hebron Barcelona Hospital Campus
dc.subject.none.fl_str_mv Endometri - Càncer
Genòmica
DISEASES::Neoplasms::Neoplasms by Site::Urogenital Neoplasms::Genital Neoplasms, Female::Uterine Neoplasms::Endometrial Neoplasms
Other subheadings::Other subheadings::Other subheadings::/genetics
ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias urogenitales::neoplasias de los genitales femeninos::neoplasias uterinas::neoplasias endometriales
Otros calificadores::Otros calificadores::Otros calificadores::/genética
topic Endometri - Càncer
Genòmica
DISEASES::Neoplasms::Neoplasms by Site::Urogenital Neoplasms::Genital Neoplasms, Female::Uterine Neoplasms::Endometrial Neoplasms
Other subheadings::Other subheadings::Other subheadings::/genetics
ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias urogenitales::neoplasias de los genitales femeninos::neoplasias uterinas::neoplasias endometriales
Otros calificadores::Otros calificadores::Otros calificadores::/genética
description Bioinformatics; Endometrial cancer; Molecular marker
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11351/7849
url https://hdl.handle.net/11351/7849
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv International Journal of Molecular Sciences;23(11)
https://doi.org/10.3390/ijms23116266
info:eu-repo/grantAgreement/ES/PE2013-2016/PI17%2F02071
info:eu-repo/grantAgreement/ES/PE2017-2020/PI20%2F01566
info:eu-repo/grantAgreement/ES/PERIS2016-2020/SLT017%2F20%2F000183
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Scientia
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869416589028229120
spelling Genomic Validation of Endometrial Cancer Patient-Derived Xenograft Models as a Preclinical ToolVillafranca Magdalena, BeatrizMasferrer Ferragutcasas, CarinaLopez Gil, CarlosColl De la Rubia, EvaRebull Santamaria, MartaParra, GenisGarcía Jiménez, AngelCabrera Diaz, SilviaColas Ortega, EvaGil Moreno, AntonioReques Llanos, ArmandoMoiola, Cristian PabloEndometri - CàncerGenòmicaDISEASES::Neoplasms::Neoplasms by Site::Urogenital Neoplasms::Genital Neoplasms, Female::Uterine Neoplasms::Endometrial NeoplasmsOther subheadings::Other subheadings::Other subheadings::/geneticsENFERMEDADES::neoplasias::neoplasias por localización::neoplasias urogenitales::neoplasias de los genitales femeninos::neoplasias uterinas::neoplasias endometrialesOtros calificadores::Otros calificadores::Otros calificadores::/genéticaBioinformatics; Endometrial cancer; Molecular markerBioinformática; Cáncer endometrial; Marcador molecularBioinformàtica; Càncer d'endometri; Marcador molecularEndometrial cancer (EC) is the second most frequent gynecological cancer worldwide. Although improvements in EC classification have enabled an accurate establishment of disease prognosis, women with a high-risk or recurrent EC face a dramatic situation due to limited further treatment options. Therefore, new strategies that closely mimic the disease are required to maximize drug development success. Patient-derived xenografts (PDXs) are widely recognized as a physiologically relevant preclinical model. Hence, we propose to molecularly and histologically validate EC PDX models. To reveal the molecular landscape of PDXs generated from 13 EC patients, we performed histological characterization and whole-exome sequencing analysis of tumor samples. We assessed the similarity between PDXs and their corresponding patient’s tumor and, additionally, to an extended cohort of EC patients obtained from The Cancer Genome Atlas (TCGA). Finally, we performed functional enrichment analysis to reveal differences in molecular pathway activation in PDX models. We demonstrated that the PDX models had a well-defined and differentiated molecular profile that matched the genomic profile described by the TCGA for each EC subtype. Thus, we validated EC PDX’s potential to reliably recapitulate the majority of histologic and molecular EC features. This work highlights the importance of a thorough characterization of preclinical models for the improvement of the success rate of drug-screening assays for personalized medicine.This research was funded by grants from the Instituto de Salud Carlos III (ISCIII) grant number PI17/02071, PI20/01566, and from the Ministerio de ciencia, Innovación y Universidades through a RETOS Colaboración (RTC-2017-6261-1), both co-financed by the European Regional Development Fund (FEDER); from Fundación Científica Asociación Española Contra el Cáncer (AECC) grant number GCTRA1804MATI, Biomedical Research Center Network (CIBERONC) grant number CB16/12/00328 and Generalitat de Catalunya, grant number 2017SGR1661. B.V.-M. is supported by a predoctoral fellowship (PERIS-SLT017/20/000183) from Generalitat de Catalunya. E.C. is supported by an Investigator Grant from AECC (INVES20051COLA).MDPIInstitut Català de la Salut[Villafranca-Magdalena B, Masferrer-Ferragutcasas C, Lopez-Gil C, Rebull M, Moiola CP] Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Escola de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Coll-de la Rubia E, Colas E] Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Escola de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. Biomedical Research Center Network (CIBERONC), Madrid, Spain. [Parra G] National Center for Genomic Analysis—Genomic Regulation Center (CNAG-CRG), Scientific Park of Barcelona, Barcelona, Spain. [García A, Reques A] Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Servei de Patologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Cabrera S, Gil-Moreno A] Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Escola de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. Biomedical Research Center Network (CIBERONC), Madrid, Spain. Servei de Ginecologia Oncològica, Vall d’Hebron Hospital Universitari, Barcelona, SpainVall d'Hebron Barcelona Hospital Campus202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/11351/7849Scientiareponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésInternational Journal of Molecular Sciences;23(11)https://doi.org/10.3390/ijms23116266info:eu-repo/grantAgreement/ES/PE2013-2016/PI17%2F02071info:eu-repo/grantAgreement/ES/PE2017-2020/PI20%2F01566info:eu-repo/grantAgreement/ES/PERIS2016-2020/SLT017%2F20%2F000183Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:11351/78492026-05-29T05:05:01Z
score 15.81155

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