Alkane-degrading properties of Dietzia sp. H0B, a key player in the Prestige oil spill biodegradation (NW Spain)

4 figuras

Detalles Bibliográficos
Autores: Alonso-Gutiérrez, Jorge, Teramoto, M., Yamazoe, A., Harayama, S., Figueras Huerta, Antonio, Novoa, Beatriz
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/47717
Acceso en línea:http://hdl.handle.net/10261/47717
Access Level:acceso abierto
Palabra clave:Alkane degradation
AlkB
Constitutive expression
CYP153
Dietzia
Oil spill
Prestige
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spelling Alkane-degrading properties of Dietzia sp. H0B, a key player in the Prestige oil spill biodegradation (NW Spain)Alonso-Gutiérrez, JorgeTeramoto, M.Yamazoe, A.Harayama, S.Figueras Huerta, AntonioNovoa, BeatrizAlkane degradationAlkBConstitutive expressionCYP153DietziaOil spillPrestige4 figurasAims: Investigation of the alkane-degrading properties of Dietzia sp. H0B, one of the isolated Corynebacterineae strains that became dominant after the Prestige oil spill. Methods and Results: Using molecular and chemical analyses, the alkanedegrading properties of strain Dietzia sp. H0B were analysed. This Grampositive isolate was able to grow on n-alkanes ranging from C12 to C38 and branched alkanes (pristane and phytane). 8-Hexadecene was detected as an intermediate of hexadecane degradation by Dietzia H0B, suggesting a novel alkane-degrading pathway in this strain. Three putative alkane hydroxylase genes (one alkB homologue and two CYP153 gene homologues of cytochrome P450 family) were PCR-amplified from Dietzia H0B and differed from previously known hydroxylase genes, which might be related to the novel degrading activity observed on Dietzia H0B. The alkane degradation activity and the alkB and CYP153 gene expression were observed constitutively regardless of the presence of the substrate, suggesting additional, novel pathways for alkane degradation. Conclusions: The results from this study suggest novel alkane-degrading pathways in Dietzia H0B and a genetic background coding for two different putative oil-degrading enzymes, which is mostly unexplored and worth to be subject of further functional analysis. Significance and Impact of the Study: This study increases the scarce information available about the genetic background of alkane degradation in genus Dietzia and suggests new pathways and novel expression mechanisms of alkane degradation.This research was supported by project VEM 2003-20068-C05-01 of the Spanish Ministerio de Educacio´n y Ciencia.Peer reviewedJohn Wiley & Sons201220122011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/47717reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1111/j.1365-2672.2011.05104.xinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/477172026-05-22T06:33:51Z
dc.title.none.fl_str_mv Alkane-degrading properties of Dietzia sp. H0B, a key player in the Prestige oil spill biodegradation (NW Spain)
title Alkane-degrading properties of Dietzia sp. H0B, a key player in the Prestige oil spill biodegradation (NW Spain)
spellingShingle Alkane-degrading properties of Dietzia sp. H0B, a key player in the Prestige oil spill biodegradation (NW Spain)
Alonso-Gutiérrez, Jorge
Alkane degradation
AlkB
Constitutive expression
CYP153
Dietzia
Oil spill
Prestige
title_short Alkane-degrading properties of Dietzia sp. H0B, a key player in the Prestige oil spill biodegradation (NW Spain)
title_full Alkane-degrading properties of Dietzia sp. H0B, a key player in the Prestige oil spill biodegradation (NW Spain)
title_fullStr Alkane-degrading properties of Dietzia sp. H0B, a key player in the Prestige oil spill biodegradation (NW Spain)
title_full_unstemmed Alkane-degrading properties of Dietzia sp. H0B, a key player in the Prestige oil spill biodegradation (NW Spain)
title_sort Alkane-degrading properties of Dietzia sp. H0B, a key player in the Prestige oil spill biodegradation (NW Spain)
dc.creator.none.fl_str_mv Alonso-Gutiérrez, Jorge
Teramoto, M.
Yamazoe, A.
Harayama, S.
Figueras Huerta, Antonio
Novoa, Beatriz
author Alonso-Gutiérrez, Jorge
author_facet Alonso-Gutiérrez, Jorge
Teramoto, M.
Yamazoe, A.
Harayama, S.
Figueras Huerta, Antonio
Novoa, Beatriz
author_role author
author2 Teramoto, M.
Yamazoe, A.
Harayama, S.
Figueras Huerta, Antonio
Novoa, Beatriz
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Alkane degradation
AlkB
Constitutive expression
CYP153
Dietzia
Oil spill
Prestige
topic Alkane degradation
AlkB
Constitutive expression
CYP153
Dietzia
Oil spill
Prestige
description 4 figuras
publishDate 2011
dc.date.none.fl_str_mv 2011
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/47717
url http://hdl.handle.net/10261/47717
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1111/j.1365-2672.2011.05104.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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