Detection of reassortant influenza B strains from 2004 to 2015 seasons in Barcelona (Catalonia, Spain) by whole genome sequencing
Influenza B viruses (FLUBV) have segmented genomes which enables the virus to evolve by segment reassortment. Since the divergence of both FLUBV lineages, B/Victoria / 2/87 (FLUBV/VIC) and B/Yamagata/16/88 (FLUBV/YAM), PB2, PB1 and HA have kept the same ancestor, while some reassortment events in th...
| Autores: | , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:286223 |
| Acceso en línea: | https://ddd.uab.cat/record/286223 https://dx.doi.org/urn:doi:10.1016/j.virusres.2023.199089 |
| Access Level: | acceso abierto |
| Palabra clave: | Influenza B viruses Reassortment event Lineage Molecular epidemiology |
| id |
ES_ae799abe1bb2cb8d6725688bcdf3d8e6 |
|---|---|
| oai_identifier_str |
oai:ddd.uab.cat:286223 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| dc.title.none.fl_str_mv |
Detection of reassortant influenza B strains from 2004 to 2015 seasons in Barcelona (Catalonia, Spain) by whole genome sequencing |
| title |
Detection of reassortant influenza B strains from 2004 to 2015 seasons in Barcelona (Catalonia, Spain) by whole genome sequencing |
| spellingShingle |
Detection of reassortant influenza B strains from 2004 to 2015 seasons in Barcelona (Catalonia, Spain) by whole genome sequencing Andrés, Cristina|||0000-0002-3200-0895 Influenza B viruses Reassortment event Lineage Molecular epidemiology |
| title_short |
Detection of reassortant influenza B strains from 2004 to 2015 seasons in Barcelona (Catalonia, Spain) by whole genome sequencing |
| title_full |
Detection of reassortant influenza B strains from 2004 to 2015 seasons in Barcelona (Catalonia, Spain) by whole genome sequencing |
| title_fullStr |
Detection of reassortant influenza B strains from 2004 to 2015 seasons in Barcelona (Catalonia, Spain) by whole genome sequencing |
| title_full_unstemmed |
Detection of reassortant influenza B strains from 2004 to 2015 seasons in Barcelona (Catalonia, Spain) by whole genome sequencing |
| title_sort |
Detection of reassortant influenza B strains from 2004 to 2015 seasons in Barcelona (Catalonia, Spain) by whole genome sequencing |
| dc.creator.none.fl_str_mv |
Andrés, Cristina|||0000-0002-3200-0895 del Cuerpo Casas, Margarita|||0000-0001-7973-5496 Rabella, Núria|||0000-0002-3409-0468 Piñana, Maria|||0000-0002-4766-2613 Iglesias Cabezas, Manuel Jesús González Sánchez, Alejandra|||0000-0002-1809-3415 Esperalba, Juliana|||0000-0003-1326-1341 Rando-Segura, Ariadna|||0000-0003-4555-7286 Martín, Maria Carmen Fuentes, Francisco Rubio, Susana Saubi, Narcis|||0000-0001-5493-1044 Pumarola Suñé, Tomàs|||0000-0002-5171-7461 Antón Pagarolas, Andrés, 1976-|||0000-0002-1476-0815 |
| author |
Andrés, Cristina|||0000-0002-3200-0895 |
| author_facet |
Andrés, Cristina|||0000-0002-3200-0895 del Cuerpo Casas, Margarita|||0000-0001-7973-5496 Rabella, Núria|||0000-0002-3409-0468 Piñana, Maria|||0000-0002-4766-2613 Iglesias Cabezas, Manuel Jesús González Sánchez, Alejandra|||0000-0002-1809-3415 Esperalba, Juliana|||0000-0003-1326-1341 Rando-Segura, Ariadna|||0000-0003-4555-7286 Martín, Maria Carmen Fuentes, Francisco Rubio, Susana Saubi, Narcis|||0000-0001-5493-1044 Pumarola Suñé, Tomàs|||0000-0002-5171-7461 Antón Pagarolas, Andrés, 1976-|||0000-0002-1476-0815 |
| author_role |
author |
| author2 |
del Cuerpo Casas, Margarita|||0000-0001-7973-5496 Rabella, Núria|||0000-0002-3409-0468 Piñana, Maria|||0000-0002-4766-2613 Iglesias Cabezas, Manuel Jesús González Sánchez, Alejandra|||0000-0002-1809-3415 Esperalba, Juliana|||0000-0003-1326-1341 Rando-Segura, Ariadna|||0000-0003-4555-7286 Martín, Maria Carmen Fuentes, Francisco Rubio, Susana Saubi, Narcis|||0000-0001-5493-1044 Pumarola Suñé, Tomàs|||0000-0002-5171-7461 Antón Pagarolas, Andrés, 1976-|||0000-0002-1476-0815 |
| author2_role |
author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Influenza B viruses Reassortment event Lineage Molecular epidemiology |
| topic |
Influenza B viruses Reassortment event Lineage Molecular epidemiology |
| description |
Influenza B viruses (FLUBV) have segmented genomes which enables the virus to evolve by segment reassortment. Since the divergence of both FLUBV lineages, B/Victoria / 2/87 (FLUBV/VIC) and B/Yamagata/16/88 (FLUBV/YAM), PB2, PB1 and HA have kept the same ancestor, while some reassortment events in the other segments have been reported worldwide. The aim of the present study was to find out reassortment episodes in FLUBV strains detected in cases attended at Hospital Universitari Vall d'Hebron and Hospital de la Santa Creu i Sant Pau (Barcelona, Spain) from 2004 to 2015 seasons. From October 2004 to May 2015, respiratory specimens were received from patients with respiratory tract infection suspicion. Influenza detection was carried out by either cell culture isolation, immunofluorescence or PCR-based assays. A RT-PCR was performed to distinguish both lineages by agarose gel electrophoresis. Whole genome amplification was performed using the universal primer set by Zhou et al. in 2012, and subsequently sequenced using Roche 454 GS Junior platform. Bioinformatic analysis was performed to characterise the sequences with B/Malaysia/2506/2007 and B/Florida/4/2006 corresponding sequences as reference of (B/VIC) and (B/YAM), respectively. A total of 118 FLUBV (75 FLUBV/VIC and 43 FLUBV/YAM), from 2004 to 2006, 2008-2011 and 2012-2015 seasons, were studied. The whole genome of 58 FLUBV/VIC and 42 FLUBV/YAM viruses was successfully amplified. Based on HA sequences, most FLUBV/VIC viruses (37; 64%) belonged to clade 1A (B/Brisbane/60/2008) except to 11 (19%), which fell within clade 1B (B/HongKong/514/2009) and 10 (17%) to B/Malaysia/2506/2004. Nine (20%) FLUBV/YAM viruses belonged to clade 2 (B/Massachusetts/02/2012), 18 (42%) to clade 3 (B/Phuket/3073/2013) and 15 (38%) fell within Florida/4/2006. Numerous intra-lineage reassortments in PB2, PB1, NA and NS were found in 2 2010-2011 viruses. An important inter-lineage reassortment event from 2008 to 2009 (11), 2010-2011 (26) and 2012-2013 (3) FLUBV/VIC (clade 1) strains to FLUBV/YAM (clade 3) was found, in addition to 1 reassortant NS in 2010-2011 B/VIC virus. Intra- and inter-lineage reassortment episodes were revealed by WGS. While PB2-PB1-HA remained in complex, NP and NS reassortant viruses were found in both lineages. Despite reassorment events are not often, the characterisation only by HA and NA sequences might be underestimating their detection. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2 2023-01-01 2023 2023-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/286223 https://dx.doi.org/urn:doi:10.1016/j.virusres.2023.199089 |
| url |
https://ddd.uab.cat/record/286223 https://dx.doi.org/urn:doi:10.1016/j.virusres.2023.199089 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI08/0118 Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 PI11/01864 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI14/01838 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0016/0003 |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.source.none.fl_str_mv |
reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
| instname_str |
Universitat Autònoma de Barcelona |
| reponame_str |
Dipòsit Digital de Documents de la UAB |
| collection |
Dipòsit Digital de Documents de la UAB |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869416565117550593 |
| spelling |
Detection of reassortant influenza B strains from 2004 to 2015 seasons in Barcelona (Catalonia, Spain) by whole genome sequencingAndrés, Cristina|||0000-0002-3200-0895del Cuerpo Casas, Margarita|||0000-0001-7973-5496Rabella, Núria|||0000-0002-3409-0468Piñana, Maria|||0000-0002-4766-2613Iglesias Cabezas, Manuel JesúsGonzález Sánchez, Alejandra|||0000-0002-1809-3415Esperalba, Juliana|||0000-0003-1326-1341Rando-Segura, Ariadna|||0000-0003-4555-7286Martín, Maria CarmenFuentes, FranciscoRubio, SusanaSaubi, Narcis|||0000-0001-5493-1044Pumarola Suñé, Tomàs|||0000-0002-5171-7461Antón Pagarolas, Andrés, 1976-|||0000-0002-1476-0815Influenza B virusesReassortment eventLineageMolecular epidemiologyInfluenza B viruses (FLUBV) have segmented genomes which enables the virus to evolve by segment reassortment. Since the divergence of both FLUBV lineages, B/Victoria / 2/87 (FLUBV/VIC) and B/Yamagata/16/88 (FLUBV/YAM), PB2, PB1 and HA have kept the same ancestor, while some reassortment events in the other segments have been reported worldwide. The aim of the present study was to find out reassortment episodes in FLUBV strains detected in cases attended at Hospital Universitari Vall d'Hebron and Hospital de la Santa Creu i Sant Pau (Barcelona, Spain) from 2004 to 2015 seasons. From October 2004 to May 2015, respiratory specimens were received from patients with respiratory tract infection suspicion. Influenza detection was carried out by either cell culture isolation, immunofluorescence or PCR-based assays. A RT-PCR was performed to distinguish both lineages by agarose gel electrophoresis. Whole genome amplification was performed using the universal primer set by Zhou et al. in 2012, and subsequently sequenced using Roche 454 GS Junior platform. Bioinformatic analysis was performed to characterise the sequences with B/Malaysia/2506/2007 and B/Florida/4/2006 corresponding sequences as reference of (B/VIC) and (B/YAM), respectively. A total of 118 FLUBV (75 FLUBV/VIC and 43 FLUBV/YAM), from 2004 to 2006, 2008-2011 and 2012-2015 seasons, were studied. The whole genome of 58 FLUBV/VIC and 42 FLUBV/YAM viruses was successfully amplified. Based on HA sequences, most FLUBV/VIC viruses (37; 64%) belonged to clade 1A (B/Brisbane/60/2008) except to 11 (19%), which fell within clade 1B (B/HongKong/514/2009) and 10 (17%) to B/Malaysia/2506/2004. Nine (20%) FLUBV/YAM viruses belonged to clade 2 (B/Massachusetts/02/2012), 18 (42%) to clade 3 (B/Phuket/3073/2013) and 15 (38%) fell within Florida/4/2006. Numerous intra-lineage reassortments in PB2, PB1, NA and NS were found in 2 2010-2011 viruses. An important inter-lineage reassortment event from 2008 to 2009 (11), 2010-2011 (26) and 2012-2013 (3) FLUBV/VIC (clade 1) strains to FLUBV/YAM (clade 3) was found, in addition to 1 reassortant NS in 2010-2011 B/VIC virus. Intra- and inter-lineage reassortment episodes were revealed by WGS. While PB2-PB1-HA remained in complex, NP and NS reassortant viruses were found in both lineages. Despite reassorment events are not often, the characterisation only by HA and NA sequences might be underestimating their detection. 22023-01-0120232023-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/286223https://dx.doi.org/urn:doi:10.1016/j.virusres.2023.199089reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI08/0118Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 PI11/01864Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI14/01838Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0016/0003open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2862232026-06-06T12:50:31Z |
| score |
15.300724 |