Cyclosporine A-induced apoptosis in renal tubular cells is related to oxidative damage and mitochondrial fission

22 p.

Bibliographic Details
Authors: Arriba De La Fuente, Gabriel De|||0000-0001-6626-623X, Calvino Fernández, Mirian, Benito Martínez, Selma|||0000-0002-3118-0564, Parra Cid, Trinidad
Format: article
Publication Date:2013
Country:España
Institution:Universidad de Alcalá (UAH)
Repository:e_Buah Biblioteca Digital Universidad de Alcalá
Language:English
OAI Identifier:oai:ebuah.uah.es:10017/59599
Online Access:http://hdl.handle.net/10017/59599
https://dx.doi.org/10.1016/j.toxlet.2013.01.007
Access Level:Open access
Keyword:Cyclosporine
Reactive oxygen species
Mitochondrial fission
Mitochondrial fusion
Apoptosis
Medicina
Medicine
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spelling Cyclosporine A-induced apoptosis in renal tubular cells is related to oxidative damage and mitochondrial fissionArriba De La Fuente, Gabriel De|||0000-0001-6626-623XCalvino Fernández, MirianBenito Martínez, Selma|||0000-0002-3118-0564Parra Cid, TrinidadCyclosporineReactive oxygen speciesMitochondrial fissionMitochondrial fusionApoptosisMedicinaMedicine22 p.Cyclosporine A (CsA) nephrotoxicity has been linked to reactive oxygen species (ROS) production in renal cells. We have demonstrated that the antioxidant Vitamin E (Vit E) abolished renal toxicity in vivo and in vitro models. As one of the main sources of intracellular ROS are mitochondria, we studied the effects of CsA on several mitochondrial functions in LLC-PK1 cells. CsA induced ROS synthesis and decreased reduced glutathione (GSH). The drug decreased mitochondrial membrane potential (m) and induced physiological modifications in both the inner (IMM) and the outer mitochondrial membranes (OMM). In the IMM, CsA provoked mitochondrial permeability tran-sition pores (MPTP) and cytochrome c was liberated into the intermembrane space. CsA also induced pore formation in the OMM, allowing that intermembrane space contents can reach cytosol. Furthermore, CsA altered the mitochondrial dynamics, inducing an increase in mitochondrial fission; CsA increased the expression of dynamin related protein 1 (Drp1) that contributes to mitochondrial fission, and decreased the expression of mitofusin 2 (Mfn2) and optic atrophy protein 1 (Opa1), proteins involved in the fusion process. All these phenomena were related to apoptosis. These effects were inhibited when cells were treated with the antioxidant Vit E suggesting that they were mediated by the synthesis of ROS.Junta de Comunidades de Castilla la ManchaInstituto de Salud Carlos III20132013-06-21journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/59599https://dx.doi.org/10.1016/j.toxlet.2013.01.007reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengJunta de Comunidades de Castilla-La Mancha http://dx.doi.org/10.13039/501100011698 Not available Not availableJunta de Comunidades de Castilla-La Mancha http://dx.doi.org/10.13039/501100011698 Not available Not availableNot available Not available Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/595992026-06-18T11:13:07Z
dc.title.none.fl_str_mv Cyclosporine A-induced apoptosis in renal tubular cells is related to oxidative damage and mitochondrial fission
title Cyclosporine A-induced apoptosis in renal tubular cells is related to oxidative damage and mitochondrial fission
spellingShingle Cyclosporine A-induced apoptosis in renal tubular cells is related to oxidative damage and mitochondrial fission
Arriba De La Fuente, Gabriel De|||0000-0001-6626-623X
Cyclosporine
Reactive oxygen species
Mitochondrial fission
Mitochondrial fusion
Apoptosis
Medicina
Medicine
title_short Cyclosporine A-induced apoptosis in renal tubular cells is related to oxidative damage and mitochondrial fission
title_full Cyclosporine A-induced apoptosis in renal tubular cells is related to oxidative damage and mitochondrial fission
title_fullStr Cyclosporine A-induced apoptosis in renal tubular cells is related to oxidative damage and mitochondrial fission
title_full_unstemmed Cyclosporine A-induced apoptosis in renal tubular cells is related to oxidative damage and mitochondrial fission
title_sort Cyclosporine A-induced apoptosis in renal tubular cells is related to oxidative damage and mitochondrial fission
dc.creator.none.fl_str_mv Arriba De La Fuente, Gabriel De|||0000-0001-6626-623X
Calvino Fernández, Mirian
Benito Martínez, Selma|||0000-0002-3118-0564
Parra Cid, Trinidad
author Arriba De La Fuente, Gabriel De|||0000-0001-6626-623X
author_facet Arriba De La Fuente, Gabriel De|||0000-0001-6626-623X
Calvino Fernández, Mirian
Benito Martínez, Selma|||0000-0002-3118-0564
Parra Cid, Trinidad
author_role author
author2 Calvino Fernández, Mirian
Benito Martínez, Selma|||0000-0002-3118-0564
Parra Cid, Trinidad
author2_role author
author
author
dc.subject.none.fl_str_mv Cyclosporine
Reactive oxygen species
Mitochondrial fission
Mitochondrial fusion
Apoptosis
Medicina
Medicine
topic Cyclosporine
Reactive oxygen species
Mitochondrial fission
Mitochondrial fusion
Apoptosis
Medicina
Medicine
description 22 p.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-06-21
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/59599
https://dx.doi.org/10.1016/j.toxlet.2013.01.007
url http://hdl.handle.net/10017/59599
https://dx.doi.org/10.1016/j.toxlet.2013.01.007
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Junta de Comunidades de Castilla-La Mancha http://dx.doi.org/10.13039/501100011698 Not available Not available
Junta de Comunidades de Castilla-La Mancha http://dx.doi.org/10.13039/501100011698 Not available Not available
Not available Not available Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
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