A dual regulatory role for the arbuscular mycorrhizal master regulator RAM1 in tomato
The REQUIRED FOR ARBUSCULAR MYCORRHIZATION1 (RAM1) transcription factor from the GRAS family is well known for its role as a master regulator of the arbuscular mycorrhizal (AM) symbiosis in dicotyledonous and monocotyledonous species, being essential in transcriptional reprogramming for the developm...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/371695 |
| Acceso en línea: | http://hdl.handle.net/10261/371695 |
| Access Level: | acceso abierto |
| Palabra clave: | Micorriza arbuscular GRAS RAM1 Estrigolactonas Tomate Regulación transcripcional |
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A dual regulatory role for the arbuscular mycorrhizal master regulator RAM1 in tomatoHo-Plágaro, TaniaTamayo Navarrete, María IsabelZeljković, S. C.Tarkowski, P.García-Garrido, José MMicorriza arbuscularGRASRAM1EstrigolactonasTomateRegulación transcripcionalThe REQUIRED FOR ARBUSCULAR MYCORRHIZATION1 (RAM1) transcription factor from the GRAS family is well known for its role as a master regulator of the arbuscular mycorrhizal (AM) symbiosis in dicotyledonous and monocotyledonous species, being essential in transcriptional reprogramming for the development and functionality of the arbuscules. In tomato, SlGRAS27 is the putative orthologue of RAM1 (here named SlRAM1), but has not yet been characterized. A reduced colonization of the root and impaired arbuscule formation were observed in SlRAM1-silenced plants, confirming the functional conservation of the RAM1 orthologue in tomato. However, unexpectedly, SlRAM1overexpressing (UBIL:SlRAM1) plants also showed decreased mycorrhizal colonization. Analysis of non-mycorrhizal UBIL:SlRAM1 roots revealed an overall regulation of AM-related genes and a reduction of strigolactone biosynthesis. Moreover, external application of the strigolactone analogue GR24 almost completely reversed the negative effects of SlRAM1 overexpression on the frequency of mycorrhization. However, it only partially recovered the pattern of arbuscule distribution observed in control plants. Our results strongly suggest that SlRAM1 has a dual regulatory role during mycorrhization and, in addition to its recognized action as a positive regulator of arbuscule development, it is also involved in different mechanisms for the negative regulation of mycorrhization, including the repression of strigolactone biosynthesis.El proyecto TH-P recibió el apoyo de una beca de investigación de la JUNTA DE ANDALUCÍA (subvención n.º POSTDOC_21_00398). Este estudio recibió el apoyo de la subvención PID2020-115336GB-I00 financiada por MCIN/AEI//10.13039/501100011033, así como del proyecto MZE-RO0423 financiado por el Ministerio de Agricultura de la República Checa y del proyecto "Las plantas como herramienta para el desarrollo global sostenible" (CZ.02.1.01/0.0/0.0/16_019/0000827) dentro del programa de Investigación, Desarrollo y Educación (OP RDE).Oxford University PressJunta de AndalucíaMinisterio de Ciencia e Innovación (España)Ministry of Agriculture of the Czech RepublicConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2024202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/371695reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115336GB-I00Ho-Plágaro, Tania; Tamayo Navarrete, María Isabel; Cavar, Sanja; Tarkowski, Petr; García-Garrido, J. M.; 2024; A dual regulatory role of the arbuscular mycorrhizal master regulator RAM1 in tomato [Dataset]; Oxford University Press; http://dx.doi.org/10.1093/jxb/erae210http://dx.doi.org/10.1093/jxb/erae210Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3716952026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
A dual regulatory role for the arbuscular mycorrhizal master regulator RAM1 in tomato |
| title |
A dual regulatory role for the arbuscular mycorrhizal master regulator RAM1 in tomato |
| spellingShingle |
A dual regulatory role for the arbuscular mycorrhizal master regulator RAM1 in tomato Ho-Plágaro, Tania Micorriza arbuscular GRAS RAM1 Estrigolactonas Tomate Regulación transcripcional |
| title_short |
A dual regulatory role for the arbuscular mycorrhizal master regulator RAM1 in tomato |
| title_full |
A dual regulatory role for the arbuscular mycorrhizal master regulator RAM1 in tomato |
| title_fullStr |
A dual regulatory role for the arbuscular mycorrhizal master regulator RAM1 in tomato |
| title_full_unstemmed |
A dual regulatory role for the arbuscular mycorrhizal master regulator RAM1 in tomato |
| title_sort |
A dual regulatory role for the arbuscular mycorrhizal master regulator RAM1 in tomato |
| dc.creator.none.fl_str_mv |
Ho-Plágaro, Tania Tamayo Navarrete, María Isabel Zeljković, S. C. Tarkowski, P. García-Garrido, José M |
| author |
Ho-Plágaro, Tania |
| author_facet |
Ho-Plágaro, Tania Tamayo Navarrete, María Isabel Zeljković, S. C. Tarkowski, P. García-Garrido, José M |
| author_role |
author |
| author2 |
Tamayo Navarrete, María Isabel Zeljković, S. C. Tarkowski, P. García-Garrido, José M |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Junta de Andalucía Ministerio de Ciencia e Innovación (España) Ministry of Agriculture of the Czech Republic Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Micorriza arbuscular GRAS RAM1 Estrigolactonas Tomate Regulación transcripcional |
| topic |
Micorriza arbuscular GRAS RAM1 Estrigolactonas Tomate Regulación transcripcional |
| description |
The REQUIRED FOR ARBUSCULAR MYCORRHIZATION1 (RAM1) transcription factor from the GRAS family is well known for its role as a master regulator of the arbuscular mycorrhizal (AM) symbiosis in dicotyledonous and monocotyledonous species, being essential in transcriptional reprogramming for the development and functionality of the arbuscules. In tomato, SlGRAS27 is the putative orthologue of RAM1 (here named SlRAM1), but has not yet been characterized. A reduced colonization of the root and impaired arbuscule formation were observed in SlRAM1-silenced plants, confirming the functional conservation of the RAM1 orthologue in tomato. However, unexpectedly, SlRAM1overexpressing (UBIL:SlRAM1) plants also showed decreased mycorrhizal colonization. Analysis of non-mycorrhizal UBIL:SlRAM1 roots revealed an overall regulation of AM-related genes and a reduction of strigolactone biosynthesis. Moreover, external application of the strigolactone analogue GR24 almost completely reversed the negative effects of SlRAM1 overexpression on the frequency of mycorrhization. However, it only partially recovered the pattern of arbuscule distribution observed in control plants. Our results strongly suggest that SlRAM1 has a dual regulatory role during mycorrhization and, in addition to its recognized action as a positive regulator of arbuscule development, it is also involved in different mechanisms for the negative regulation of mycorrhization, including the repression of strigolactone biosynthesis. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 2024 2024 2024 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/371695 |
| url |
http://hdl.handle.net/10261/371695 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115336GB-I00 Ho-Plágaro, Tania; Tamayo Navarrete, María Isabel; Cavar, Sanja; Tarkowski, Petr; García-Garrido, J. M.; 2024; A dual regulatory role of the arbuscular mycorrhizal master regulator RAM1 in tomato [Dataset]; Oxford University Press; http://dx.doi.org/10.1093/jxb/erae210 http://dx.doi.org/10.1093/jxb/erae210 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Oxford University Press |
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Oxford University Press |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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