Analysis of a HAM1 partial loss-of-function mutant in different contexts
(A) Representative pictures under white light of infected and non-treated N. benthamiana plants at 11 days post-inoculation. White bar = 4 cm. (B) Representative pictures under white light of upper non-inoculated leaves, at 40 days post-inoculation (dpi), of cassava plants inoculated with the indica...
| Autores: | , , , , , , , , , , |
|---|---|
| Tipo de recurso: | conjunto de datos |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/310824 |
| Acceso en línea: | http://hdl.handle.net/10261/310824 |
| Access Level: | acceso abierto |
| Palabra clave: | Unexpected high concentration Infectious cdna clone Cbsd ), dubbed Optimizes viral fitness Atypical viral factor Nicotiana benthamiana Mutant viruses indicated Dependent rna polymerase Ms experiments showed Div><p Family expressing maf Viral rna Rna viruses Potyviridae Two viruses Ebola Yet established Widespread pyrophosphatase Ucbsv requires Specific partners Specific constraint Serious threat Reverse genetics Quite likely Plants One secoviridae Model plant Living organisms Like pyrophosphatases Like proteins Like family Incredibly versatile Food security First example Feature shared Experimental evolution Economic impacts Canonical nucleotides Antiviral defense Another member Africa caused http://metadata.un.org/sdg/2 End hunger, achieve food security and improved nutrition and promote sustainable agriculture |
| Sumario: | (A) Representative pictures under white light of infected and non-treated N. benthamiana plants at 11 days post-inoculation. White bar = 4 cm. (B) Representative pictures under white light of upper non-inoculated leaves, at 40 days post-inoculation (dpi), of cassava plants inoculated with the indicated viruses. White arrows indicate the presence of leaf chlorosis induced by UCBSV in cassava. White bar = 4 cm. (C) Representative pictures under white light of stems, at 90 dpi, of cassava plants inoculated with the indicated viruses. Black arrows indicate the presence of brown streaks induced by UCBSV in cassava. (D) Chromatograms of Sanger sequencing results of the DNA fragment of interest amplified by RT-PCR. RNA samples deriving from upper non-inoculated leaves of the indicated cassava plant inoculated with UCBSV-HAM1N35A were used as template. Leaves for RNA preparation were harvested at both 90 and 120 days post-infection (dpi). Coloured circles surround both the original and the naturally introduced mutation. (E) Cropped polyacrylamide gel stained with coomasie blue that shows that equivalent amounts of the indicated proteins were used for the experiment in Fig 4E. 1:20 dilutions (10, 20 and 40 nanograms, correspondingly) were used for the measurement of ITPase activity. |
|---|