Mapping Neuron–Astrocyte Circuits with CaMPARIGFAP
CaMPARI<inf>GFAP</inf>, a calcium-modulated photoactivatable sensor, is a novel optical tool implemented for astrocytes that allows real-time monitoring of astrocytic calcium dynamics in parallel with targeting Ca<sup>2+</sup> activity in large areas of tissue with temporal p...
| Authors: | , |
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| Format: | other |
| Status: | Published version |
| Publication Date: | 2024 |
| Country: | España |
| Institution: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repository: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/395812 |
| Online Access: | http://hdl.handle.net/10261/395812 https://api.elsevier.com/content/abstract/scopus_id/85201941123 |
| Access Level: | Open access |
| Keyword: | Astrocyte spatial activity Calcium-modulated Photoactivatable imaging Optogenetic stimulation Neuron–astrocyte circuits http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages |
| Summary: | CaMPARI<inf>GFAP</inf>, a calcium-modulated photoactivatable sensor, is a novel optical tool implemented for astrocytes that allows real-time monitoring of astrocytic calcium dynamics in parallel with targeting Ca<sup>2+</sup> activity in large areas of tissue with temporal precision. In this chapter, we provide detailed guidelines to map neuron–astrocyte circuits in entire brain regions by combining CaMPARI<inf>GFAP</inf> with opsin-based protocols to activate specific neuronal pathways, establishing a methodology to expand our understanding of astrocyte involvement in complex circuits. |
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