Sex and tissue specific gene expression patterns identified following de novo transcriptomic analysis of the Norway lobster, Nephrops norvegicus

[Background] The Norway lobster, Nephrops norvegicus, is economically important in European fisheries and is a key organism in local marine ecosystems. Despite multi-faceted scientific interest in this species, our current knowledge of genetic resources in this species remains very limited. Here, we...

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Detalles Bibliográficos
Autores: Rotllant, Guiomar, Sbragaglia, Valerio, Company, Joan B., Aguzzi, Jacopo, Mather, Peter B.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/154198
Acceso en línea:http://hdl.handle.net/10261/154198
Access Level:acceso abierto
Palabra clave:Norway lobster
Nephrops norvegicus
Gene expression
Reproduction
RNA-Seq
Sex-specific expression
Sex-biased expression
Transcriptomes
RNA
Descripción
Sumario:[Background] The Norway lobster, Nephrops norvegicus, is economically important in European fisheries and is a key organism in local marine ecosystems. Despite multi-faceted scientific interest in this species, our current knowledge of genetic resources in this species remains very limited. Here, we generated a reference de novo transcriptome for N. norvegicus from multiple tissues in both sexes. Bioinformatic analyses were conducted to detect transcripts that were expressed exclusively in either males or females. Patterns were validated via RT-PCR.