Resveratrol and Pterostilbene, Two Analogue Phenolic Compounds, Affect Aquaglyceroporin Expression in a Different Manner in Adipose Tissue

Aquaglyceroporins (AQPs) are transmembrane channels that mediate glycerol release and glycerol uptake. They are involved in fat metabolism, with implications in obesity. The aim was to determine whether the administration of resveratrol and pterostilbene during the six weeks of the experimental peri...

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Autores: Gómez Zorita, Saioa, Trepiana Arin, Jenifer, Fernández Quintela, Alfredo, González, Marcela, Portillo Baquedano, María Puy
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/30291
Acceso en línea:http://hdl.handle.net/10810/30291
Access Level:acceso abierto
Palabra clave:aquaglyceroporins
white adipose tissue
brown adipose tissue
resveratrol
pterostilbene
high-fat diet
glycerol permeability
insulin-resistance
gene-expression
induced obesity
mice
metabolism
rats
aquaporin-7
adipocytes
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oai_identifier_str oai:addi.ehu.eus:10810/30291
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repository_id_str
spelling Resveratrol and Pterostilbene, Two Analogue Phenolic Compounds, Affect Aquaglyceroporin Expression in a Different Manner in Adipose TissueGómez Zorita, SaioaTrepiana Arin, JeniferFernández Quintela, AlfredoGonzález, MarcelaPortillo Baquedano, María Puyaquaglyceroporinswhite adipose tissuebrown adipose tissueresveratrolpterostilbenehigh-fat dietglycerol permeabilityinsulin-resistancegene-expressioninduced obesitymicemetabolismratsaquaporin-7adipocytesAquaglyceroporins (AQPs) are transmembrane channels that mediate glycerol release and glycerol uptake. They are involved in fat metabolism, with implications in obesity. The aim was to determine whether the administration of resveratrol and pterostilbene during the six weeks of the experimental period would modify AQPs expression in white and brown adipose tissues from Wistar rats fed an obesogenic diet, and to establish a potential relationship with the delipidating properties of these compounds. Consequently, thirty-six rats were divided into four groups: (a) group fed a standard diet; and three more groups fed a high-fat high-sucrose diet: (b) high-fat high-sucrose group: (c) pterostilbene-treated group (30 mg/kg/d): (d) resveratrol-treated group (30 mg/kg/d). Epididymal, subcutaneous white adipose tissues and interscapular brown adipose tissue were dissected. AQPs gene expression (RT-PCR) and protein expression (western-blot) were measured. In white adipose tissue, pterostilbene reduced subcutaneous adipose tissue weight and prevented the decrease in AQP9 induced by obesogenic feeding, and thus glycerol uptake for triglyceride accumulation. Resveratrol reduced epididymal adipose tissue weight and avoided the decrease in AQPs related to glycerol release induced by high-fat high-sucrose feeding, suggesting the involvement of lipolysis in its body-fat lowering effect. Regarding brown adipose tissue, AQP7 seemed not to be involved in the previously reported thermogenic activity of both phenolic compounds.This research has been supported by MINECO (AGL-2015-65719), Instituto de Salud Carlos III (CIBERobn) and Basque Government (IT-572-13; PA18/03).MDPI201820182018info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/30291reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MINECO/AGL-2015-65719/https://www.mdpi.com/1422-0067/19/9/2654info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).Atribución 3.0 Españaoai:addi.ehu.eus:10810/302912026-06-18T09:23:17Z
dc.title.none.fl_str_mv Resveratrol and Pterostilbene, Two Analogue Phenolic Compounds, Affect Aquaglyceroporin Expression in a Different Manner in Adipose Tissue
title Resveratrol and Pterostilbene, Two Analogue Phenolic Compounds, Affect Aquaglyceroporin Expression in a Different Manner in Adipose Tissue
spellingShingle Resveratrol and Pterostilbene, Two Analogue Phenolic Compounds, Affect Aquaglyceroporin Expression in a Different Manner in Adipose Tissue
Gómez Zorita, Saioa
aquaglyceroporins
white adipose tissue
brown adipose tissue
resveratrol
pterostilbene
high-fat diet
glycerol permeability
insulin-resistance
gene-expression
induced obesity
mice
metabolism
rats
aquaporin-7
adipocytes
title_short Resveratrol and Pterostilbene, Two Analogue Phenolic Compounds, Affect Aquaglyceroporin Expression in a Different Manner in Adipose Tissue
title_full Resveratrol and Pterostilbene, Two Analogue Phenolic Compounds, Affect Aquaglyceroporin Expression in a Different Manner in Adipose Tissue
title_fullStr Resveratrol and Pterostilbene, Two Analogue Phenolic Compounds, Affect Aquaglyceroporin Expression in a Different Manner in Adipose Tissue
title_full_unstemmed Resveratrol and Pterostilbene, Two Analogue Phenolic Compounds, Affect Aquaglyceroporin Expression in a Different Manner in Adipose Tissue
title_sort Resveratrol and Pterostilbene, Two Analogue Phenolic Compounds, Affect Aquaglyceroporin Expression in a Different Manner in Adipose Tissue
dc.creator.none.fl_str_mv Gómez Zorita, Saioa
Trepiana Arin, Jenifer
Fernández Quintela, Alfredo
González, Marcela
Portillo Baquedano, María Puy
author Gómez Zorita, Saioa
author_facet Gómez Zorita, Saioa
Trepiana Arin, Jenifer
Fernández Quintela, Alfredo
González, Marcela
Portillo Baquedano, María Puy
author_role author
author2 Trepiana Arin, Jenifer
Fernández Quintela, Alfredo
González, Marcela
Portillo Baquedano, María Puy
author2_role author
author
author
author
dc.subject.none.fl_str_mv aquaglyceroporins
white adipose tissue
brown adipose tissue
resveratrol
pterostilbene
high-fat diet
glycerol permeability
insulin-resistance
gene-expression
induced obesity
mice
metabolism
rats
aquaporin-7
adipocytes
topic aquaglyceroporins
white adipose tissue
brown adipose tissue
resveratrol
pterostilbene
high-fat diet
glycerol permeability
insulin-resistance
gene-expression
induced obesity
mice
metabolism
rats
aquaporin-7
adipocytes
description Aquaglyceroporins (AQPs) are transmembrane channels that mediate glycerol release and glycerol uptake. They are involved in fat metabolism, with implications in obesity. The aim was to determine whether the administration of resveratrol and pterostilbene during the six weeks of the experimental period would modify AQPs expression in white and brown adipose tissues from Wistar rats fed an obesogenic diet, and to establish a potential relationship with the delipidating properties of these compounds. Consequently, thirty-six rats were divided into four groups: (a) group fed a standard diet; and three more groups fed a high-fat high-sucrose diet: (b) high-fat high-sucrose group: (c) pterostilbene-treated group (30 mg/kg/d): (d) resveratrol-treated group (30 mg/kg/d). Epididymal, subcutaneous white adipose tissues and interscapular brown adipose tissue were dissected. AQPs gene expression (RT-PCR) and protein expression (western-blot) were measured. In white adipose tissue, pterostilbene reduced subcutaneous adipose tissue weight and prevented the decrease in AQP9 induced by obesogenic feeding, and thus glycerol uptake for triglyceride accumulation. Resveratrol reduced epididymal adipose tissue weight and avoided the decrease in AQPs related to glycerol release induced by high-fat high-sucrose feeding, suggesting the involvement of lipolysis in its body-fat lowering effect. Regarding brown adipose tissue, AQP7 seemed not to be involved in the previously reported thermogenic activity of both phenolic compounds.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/30291
url http://hdl.handle.net/10810/30291
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/AGL-2015-65719/
https://www.mdpi.com/1422-0067/19/9/2654
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300724

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