Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus

The endocannabinoid system modulates synaptic plasticity in the hippocampus, but a link between long-term synaptic plasticity and the type 1 cannabinoid (CB1) receptor at medial perforant path (MPP) synapses remains elusive. Here, immuno-electron microscopy in adult mice showed that similar to 26% o...

Descripción completa

Detalles Bibliográficos
Autores: Peñasco Iglesias, Sara, Rico Barrio, Irantzu, Puente Bustinza, Nagore, Gómez Urquijo, Sonia María, Fontaine, Christine J., Egaña Huguet, Jon, Achicallende Urcaregui, Svein, Ramos Uriarte, Almudena, Reguero Acebal, Leire, Elezgarai Gabantxo, Izaskun, Nahirney, Patrick C., Christie, Brian R., Grandes Moreno, Pedro Rolando
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/41425
Acceso en línea:http://hdl.handle.net/10810/41425
Access Level:acceso abierto
Palabra clave:cb
receptor
2-ag
electrophysiology
excitatory synapses
long-term depression
hippocampus
cannabinoid receptor
plasticity
system
activation
memory
nmda
cb1
inhibition
object
Descripción
Sumario:The endocannabinoid system modulates synaptic plasticity in the hippocampus, but a link between long-term synaptic plasticity and the type 1 cannabinoid (CB1) receptor at medial perforant path (MPP) synapses remains elusive. Here, immuno-electron microscopy in adult mice showed that similar to 26% of the excitatory synaptic terminals in the middle 1/3 of the dentate molecular layer (DML) contained CB1 receptors, and field excitatory postsynaptic potentials evoked by MPP stimulation were inhibited by CB1 receptor activation. In addition, MPP stimulation at 10 Hz for 10 min triggered CB, receptor-dependent excitatory long-term depression (eCB-eLTD) at MPP synapses of wild-type mice but not on CB1-knockout mice. This eCB-eLTD was group I mGluR-dependent, required intracellular calcium influx and 2-arachydonoyl-glycerol (2-AG) synthesis but did not depend on N-methyl-d-aspartate (NMDA) receptors. Overall, these results point to a functional role for CB1 receptors with eCB-eLTD at DML MPP synapses and further involve these receptors in memory processing within the adult brain.

Similares en LA Referencia