Helpful Criteria When Implementing NGS Panels in Childhood Lymphoblastic Leukemia

The development of Next-Generation Sequencing (NGS) has provided useful diagnostic, prognostic, and therapeutic strategies for individualized management of B-cell precursor acute lymphoblastic leukemia (BCP-ALL) patients. Consequently, NGS is rapidly being established in clinical practice. However,...

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Detalles Bibliográficos
Autores: Vega-Garcia, Nerea, Benito, Rocío|||0000-0001-9781-4198, Esperanza-Cebollada, Elena|||0000-0001-5451-0370, Llop, Marta, Robledo, Cristina|||0000-0003-0445-3210, Vicente-Garcés, Clara|||0000-0002-2556-043X, Alonso, Javier|||0000-0002-6287-8391, Barragán, Eva, Fernández, Guerau, Hernández-Sánchez, Jesús M., Martín-Izquierdo, Marta, Maynou, Joan, Minguela, Alfredo|||0000-0003-2472-5893, Montaño, Adrián|||0000-0002-3178-6726, Ortega, Margarita, Torrebadell, Montserrat, Cervera, José|||0000-0001-8252-1106, Sánchez, Joaquín, Jiménez-Velasco, Antonio|||0000-0001-6421-7872, Riesco, Susana, Hernández Rivas, Jesús María|||0000-0002-9661-9371, Lassaletta, Álvaro|||0000-0003-2881-1473, Fernández, José María, Rives, Susana|||0000-0002-5658-1831, Dapena, José Luis|||0000-0002-6711-7479, Ramírez, Manuel|||0000-0003-0332-6973, Camós, Mireia|||0000-0003-3658-7942
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:253143
Acceso en línea:https://ddd.uab.cat/record/253143
https://dx.doi.org/urn:doi:10.3390/jpm10040244
Access Level:acceso abierto
Palabra clave:Next-generation sequencing
NGS-targeted panel
Childhood acute lymphoblastic leukemia
Descripción
Sumario:The development of Next-Generation Sequencing (NGS) has provided useful diagnostic, prognostic, and therapeutic strategies for individualized management of B-cell precursor acute lymphoblastic leukemia (BCP-ALL) patients. Consequently, NGS is rapidly being established in clinical practice. However, the technology's complexity, bioinformatics analysis, and the different available options difficult a broad consensus between different laboratories in its daily routine introduction. This collaborative study among Spanish centers was aimed to assess the feasibility, pros, and cons of our customized panel and other commercial alternatives of NGS-targeted approaches. The custom panel was tested in three different sequencing centers. We used the same samples to assess other commercial panels (Oncomine TM Childhood Cancer Research Assay; Archer ® FusionPlex ® ALL, and Human Comprehensive Cancer Panel GeneRead Panel v2 ®). Overall, the panels showed a good performance in different centers and platforms, but each NGS approach presented some issues, as well as pros and cons. Moreover, a previous consensus on the analysis and reporting following international guidelines would be preferable to improve the concordance in results among centers. Our study shows the challenges posed by NGS methodology and the need to consider several aspects of the chosen NGS-targeted approach and reach a consensus before implementing it in daily practice.

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