Engineering pH-Sensitive Stable Nanovesicles for Delivery of MicroRNA Therapeutics
MicroRNAs (miRNAs) are small non-coding endogenous RNAs, which are attracting a growing interest as therapeutic molecules due to their central role in major diseases. However, the transformation of these biomolecules into drugs is limited due to their unstability in the bloodstream, caused by nuclea...
| Autores: | , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Recursos: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/185015 |
| Acesso em linha: | https://hdl.handle.net/2445/185015 |
| Access Level: | acceso abierto |
| Palavra-chave: | Tumors Micro RNAs MicroRNAs |
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Engineering pH-Sensitive Stable Nanovesicles for Delivery of MicroRNA TherapeuticsBoloix, AriadnaFeiner Gracia, NataliaKober, MarinaRepetto, JavierPascarella, RosaSoriano, AroaMasanas, MarcSegovia, NathalyVargas Nadal, GuillemMerlo Mas, JosepDanino, DganitAbutbul-Ionita, InbalForadada, LaiaRoma, JosepCordoba, AlbaSala, SantiSánchez de Toledo, JosepGallego Melcón, SoledadVeciana, JaumeAlbertazzi, LorenzoSegura, Miguel F.Ventosa, NoraTumorsMicro RNAsMicroRNAsTumorsMicroRNAs (miRNAs) are small non-coding endogenous RNAs, which are attracting a growing interest as therapeutic molecules due to their central role in major diseases. However, the transformation of these biomolecules into drugs is limited due to their unstability in the bloodstream, caused by nucleases abundantly present in the blood, and poor capacity to enter cells. The conjugation of miRNAs to nanoparticles (NPs) could be an effective strategy for their clinical delivery. Herein, the engineering of non-liposomal lipid nanovesicles, named quatsomes (QS), for the delivery of miRNAs and other small RNAs into the cytosol of tumor cells, triggering a tumor-suppressive response is reported. The engineered pH-sensitive nanovesicles have controlled structure (unilamellar), size (<150 nm) and composition. These nanovesicles are colloidal stable (>24 weeks), and are prepared by a green, GMP compliant, and scalable one-step procedure, which are all unavoidable requirements for the arrival to the clinical practice of NP based miRNA therapeutics. Furthermore, QS protect miRNAs from RNAses and when injected intravenously, deliver them into liver, lung, and neuroblastoma xenografts tumors. These stable nanovesicles with tunable pH sensitiveness constitute an attractive platform for the efficient delivery of miRNAs and other small RNAs with therapeutic activity and their exploitation in the clinics.Wiley2022202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion14 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/185015Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1002/smll.202101959Small, 2022, vol. 18https://doi.org/10.1002/smll.202101959cc by (c) Boloix, Ariadna et al, 2022http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1850152026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Engineering pH-Sensitive Stable Nanovesicles for Delivery of MicroRNA Therapeutics |
| title |
Engineering pH-Sensitive Stable Nanovesicles for Delivery of MicroRNA Therapeutics |
| spellingShingle |
Engineering pH-Sensitive Stable Nanovesicles for Delivery of MicroRNA Therapeutics Boloix, Ariadna Tumors Micro RNAs MicroRNAs Tumors |
| title_short |
Engineering pH-Sensitive Stable Nanovesicles for Delivery of MicroRNA Therapeutics |
| title_full |
Engineering pH-Sensitive Stable Nanovesicles for Delivery of MicroRNA Therapeutics |
| title_fullStr |
Engineering pH-Sensitive Stable Nanovesicles for Delivery of MicroRNA Therapeutics |
| title_full_unstemmed |
Engineering pH-Sensitive Stable Nanovesicles for Delivery of MicroRNA Therapeutics |
| title_sort |
Engineering pH-Sensitive Stable Nanovesicles for Delivery of MicroRNA Therapeutics |
| dc.creator.none.fl_str_mv |
Boloix, Ariadna Feiner Gracia, Natalia Kober, Marina Repetto, Javier Pascarella, Rosa Soriano, Aroa Masanas, Marc Segovia, Nathaly Vargas Nadal, Guillem Merlo Mas, Josep Danino, Dganit Abutbul-Ionita, Inbal Foradada, Laia Roma, Josep Cordoba, Alba Sala, Santi Sánchez de Toledo, Josep Gallego Melcón, Soledad Veciana, Jaume Albertazzi, Lorenzo Segura, Miguel F. Ventosa, Nora |
| author |
Boloix, Ariadna |
| author_facet |
Boloix, Ariadna Feiner Gracia, Natalia Kober, Marina Repetto, Javier Pascarella, Rosa Soriano, Aroa Masanas, Marc Segovia, Nathaly Vargas Nadal, Guillem Merlo Mas, Josep Danino, Dganit Abutbul-Ionita, Inbal Foradada, Laia Roma, Josep Cordoba, Alba Sala, Santi Sánchez de Toledo, Josep Gallego Melcón, Soledad Veciana, Jaume Albertazzi, Lorenzo Segura, Miguel F. Ventosa, Nora |
| author_role |
author |
| author2 |
Feiner Gracia, Natalia Kober, Marina Repetto, Javier Pascarella, Rosa Soriano, Aroa Masanas, Marc Segovia, Nathaly Vargas Nadal, Guillem Merlo Mas, Josep Danino, Dganit Abutbul-Ionita, Inbal Foradada, Laia Roma, Josep Cordoba, Alba Sala, Santi Sánchez de Toledo, Josep Gallego Melcón, Soledad Veciana, Jaume Albertazzi, Lorenzo Segura, Miguel F. Ventosa, Nora |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Tumors Micro RNAs MicroRNAs Tumors |
| topic |
Tumors Micro RNAs MicroRNAs Tumors |
| description |
MicroRNAs (miRNAs) are small non-coding endogenous RNAs, which are attracting a growing interest as therapeutic molecules due to their central role in major diseases. However, the transformation of these biomolecules into drugs is limited due to their unstability in the bloodstream, caused by nucleases abundantly present in the blood, and poor capacity to enter cells. The conjugation of miRNAs to nanoparticles (NPs) could be an effective strategy for their clinical delivery. Herein, the engineering of non-liposomal lipid nanovesicles, named quatsomes (QS), for the delivery of miRNAs and other small RNAs into the cytosol of tumor cells, triggering a tumor-suppressive response is reported. The engineered pH-sensitive nanovesicles have controlled structure (unilamellar), size (<150 nm) and composition. These nanovesicles are colloidal stable (>24 weeks), and are prepared by a green, GMP compliant, and scalable one-step procedure, which are all unavoidable requirements for the arrival to the clinical practice of NP based miRNA therapeutics. Furthermore, QS protect miRNAs from RNAses and when injected intravenously, deliver them into liver, lung, and neuroblastoma xenografts tumors. These stable nanovesicles with tunable pH sensitiveness constitute an attractive platform for the efficient delivery of miRNAs and other small RNAs with therapeutic activity and their exploitation in the clinics. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2022 2022 2022 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/185015 |
| url |
https://hdl.handle.net/2445/185015 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: https://doi.org/10.1002/smll.202101959 Small, 2022, vol. 18 https://doi.org/10.1002/smll.202101959 |
| dc.rights.none.fl_str_mv |
cc by (c) Boloix, Ariadna et al, 2022 http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
cc by (c) Boloix, Ariadna et al, 2022 http://creativecommons.org/licenses/by/3.0/es/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
14 p. application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Wiley |
| publisher.none.fl_str_mv |
Wiley |
| dc.source.none.fl_str_mv |
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Recercat. Dipósit de la Recerca de Catalunya |
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Recercat. Dipósit de la Recerca de Catalunya |
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