Dynamics of cellular senescence markers after HCV elimination spontaneously or by DAAs in people living with HIV

Background: We identified that acute or chronic Hepatitis C (HCV) infection in people living with HIV (PLWHIV) results in different senescence profiles. However, variations in these profiles after HCV elimination, spontaneously or with direct-acting antivirals (DAAs), remain unclear. Methods: Longit...

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Autores: Lara-Aguilar, Violeta, Valle-Millares, Daniel, Crespo-Bermejo, Celia, Grande-García, Sergio, Llamas-Adán, Manuel, Cortijo-Alfonso, María Engracia, Martín-Carbonero, Luz, Domínguez, Lourdes, Ryan, Pablo, de Los Santos, Ignacio, Bartolomé-Sánchez, Sofía, Vidal-Alcántara, Erick Joan, Jimenez-Sousa, Maria Angeles, Fernandez-Rodriguez, Amanda, Briz, Veronica, Multidisciplinary HIV/Hepatitis Viral Coinfection Group (COVIHEP)
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/16051
Acceso en línea:http://hdl.handle.net/20.500.12105/16051
Access Level:acceso abierto
Palabra clave:Hepatitis C, Chronic
Hepatitis C
HIV Infections
Humans
Antiviral Agents
Cellular Senescence
Hepacivirus
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spelling Dynamics of cellular senescence markers after HCV elimination spontaneously or by DAAs in people living with HIVLara-Aguilar, VioletaValle-Millares, DanielCrespo-Bermejo, CeliaGrande-García, SergioLlamas-Adán, ManuelCortijo-Alfonso, María EngraciaMartín-Carbonero, LuzDomínguez, LourdesRyan, Pablode Los Santos, IgnacioBartolomé-Sánchez, SofíaVidal-Alcántara, Erick JoanJimenez-Sousa, Maria AngelesFernandez-Rodriguez, AmandaBriz, VeronicaMultidisciplinary HIV/Hepatitis Viral Coinfection Group (COVIHEP)Hepatitis C, ChronicHepatitis CHIV InfectionsHumansAntiviral AgentsCellular SenescenceHepacivirusBackground: We identified that acute or chronic Hepatitis C (HCV) infection in people living with HIV (PLWHIV) results in different senescence profiles. However, variations in these profiles after HCV elimination, spontaneously or with direct-acting antivirals (DAAs), remain unclear. Methods: Longitudinal observational study (48 weeks) in 70 PLWHIV: 23 PLWHIV with active HCV-chronic infection (CHC) before and after HCV eradication with DAAs, 12 PLWHIV who spontaneously clarify the HCV (SC), and 35 controls (HIV). Oxidative stress was quantified at DNA, lipid, protein, and nitrate levels, as well as the antioxidant capacity and glutathione enzyme. The replicative senescence was evaluated by relative telomere length measurement by PCR and twenty-six factors related to Senescence-Associated Secretory Phenotype (SASP) were characterized by Luminex. Differences in senescence markers was evaluated by generalized linear models. Results: During follow-up, the SC group achieved a significant improvement in glutathione enzyme and lipid peroxidation. The secretion of SASP markers increased but was still lower than that of the HIV group. Overall, the CHC group reduced the levels of oxidative stress and SASP markers to levels like those of the HIV group. No significant differences in telomere shortening were observed between groups. Conclusions: As the time since spontaneous resolution of HCV infection increased, patients had an improved senescence profile compared to the HIV group. Elimination of chronic HCV infection by DAAs led to a partial improvement of the senescent profile by restoring oxidative stress levels. However, although some SASP markers reached levels like those of the HIV group, others remained altered.ElsevierMinisterio de Ciencia e Innovación (España)Instituto de Salud Carlos IIIFundación Universidad Alfonso X el SabioBanco SantanderCentro de Investigación Biomédica en Red - CIBERINFEC (Enfermedades Infecciosas)20232023-05-1120232023-06-0120232023-06-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/20.500.12105/16051reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/160512026-06-12T12:43:37Z
dc.title.none.fl_str_mv Dynamics of cellular senescence markers after HCV elimination spontaneously or by DAAs in people living with HIV
title Dynamics of cellular senescence markers after HCV elimination spontaneously or by DAAs in people living with HIV
spellingShingle Dynamics of cellular senescence markers after HCV elimination spontaneously or by DAAs in people living with HIV
Lara-Aguilar, Violeta
Hepatitis C, Chronic
Hepatitis C
HIV Infections
Humans
Antiviral Agents
Cellular Senescence
Hepacivirus
title_short Dynamics of cellular senescence markers after HCV elimination spontaneously or by DAAs in people living with HIV
title_full Dynamics of cellular senescence markers after HCV elimination spontaneously or by DAAs in people living with HIV
title_fullStr Dynamics of cellular senescence markers after HCV elimination spontaneously or by DAAs in people living with HIV
title_full_unstemmed Dynamics of cellular senescence markers after HCV elimination spontaneously or by DAAs in people living with HIV
title_sort Dynamics of cellular senescence markers after HCV elimination spontaneously or by DAAs in people living with HIV
dc.creator.none.fl_str_mv Lara-Aguilar, Violeta
Valle-Millares, Daniel
Crespo-Bermejo, Celia
Grande-García, Sergio
Llamas-Adán, Manuel
Cortijo-Alfonso, María Engracia
Martín-Carbonero, Luz
Domínguez, Lourdes
Ryan, Pablo
de Los Santos, Ignacio
Bartolomé-Sánchez, Sofía
Vidal-Alcántara, Erick Joan
Jimenez-Sousa, Maria Angeles
Fernandez-Rodriguez, Amanda
Briz, Veronica
Multidisciplinary HIV/Hepatitis Viral Coinfection Group (COVIHEP)
author Lara-Aguilar, Violeta
author_facet Lara-Aguilar, Violeta
Valle-Millares, Daniel
Crespo-Bermejo, Celia
Grande-García, Sergio
Llamas-Adán, Manuel
Cortijo-Alfonso, María Engracia
Martín-Carbonero, Luz
Domínguez, Lourdes
Ryan, Pablo
de Los Santos, Ignacio
Bartolomé-Sánchez, Sofía
Vidal-Alcántara, Erick Joan
Jimenez-Sousa, Maria Angeles
Fernandez-Rodriguez, Amanda
Briz, Veronica
Multidisciplinary HIV/Hepatitis Viral Coinfection Group (COVIHEP)
author_role author
author2 Valle-Millares, Daniel
Crespo-Bermejo, Celia
Grande-García, Sergio
Llamas-Adán, Manuel
Cortijo-Alfonso, María Engracia
Martín-Carbonero, Luz
Domínguez, Lourdes
Ryan, Pablo
de Los Santos, Ignacio
Bartolomé-Sánchez, Sofía
Vidal-Alcántara, Erick Joan
Jimenez-Sousa, Maria Angeles
Fernandez-Rodriguez, Amanda
Briz, Veronica
Multidisciplinary HIV/Hepatitis Viral Coinfection Group (COVIHEP)
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Instituto de Salud Carlos III
Fundación Universidad Alfonso X el Sabio
Banco Santander
Centro de Investigación Biomédica en Red - CIBERINFEC (Enfermedades Infecciosas)
dc.subject.none.fl_str_mv Hepatitis C, Chronic
Hepatitis C
HIV Infections
Humans
Antiviral Agents
Cellular Senescence
Hepacivirus
topic Hepatitis C, Chronic
Hepatitis C
HIV Infections
Humans
Antiviral Agents
Cellular Senescence
Hepacivirus
description Background: We identified that acute or chronic Hepatitis C (HCV) infection in people living with HIV (PLWHIV) results in different senescence profiles. However, variations in these profiles after HCV elimination, spontaneously or with direct-acting antivirals (DAAs), remain unclear. Methods: Longitudinal observational study (48 weeks) in 70 PLWHIV: 23 PLWHIV with active HCV-chronic infection (CHC) before and after HCV eradication with DAAs, 12 PLWHIV who spontaneously clarify the HCV (SC), and 35 controls (HIV). Oxidative stress was quantified at DNA, lipid, protein, and nitrate levels, as well as the antioxidant capacity and glutathione enzyme. The replicative senescence was evaluated by relative telomere length measurement by PCR and twenty-six factors related to Senescence-Associated Secretory Phenotype (SASP) were characterized by Luminex. Differences in senescence markers was evaluated by generalized linear models. Results: During follow-up, the SC group achieved a significant improvement in glutathione enzyme and lipid peroxidation. The secretion of SASP markers increased but was still lower than that of the HIV group. Overall, the CHC group reduced the levels of oxidative stress and SASP markers to levels like those of the HIV group. No significant differences in telomere shortening were observed between groups. Conclusions: As the time since spontaneous resolution of HCV infection increased, patients had an improved senescence profile compared to the HIV group. Elimination of chronic HCV infection by DAAs led to a partial improvement of the senescent profile by restoring oxidative stress levels. However, although some SASP markers reached levels like those of the HIV group, others remained altered.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-05-11
2023
2023-06-01
2023
2023-06-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/16051
url http://hdl.handle.net/20.500.12105/16051
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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