Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients

Mycobacterium abscessus is an extensively drug-resistant pathogen that causes pulmonary disease, particularly in cystic fibrosis (CF) patients. Identifying direct patient-to-patient transmission of M. abscessus is critically important in directing an infection control policy for the management of ri...

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Autores: Doyle, Ronan M.|||0000-0001-5001-1945, Rubio, Marc|||0000-0002-4811-0642, Dixon, Garth|||0000-0001-8165-3094, Hartley, John, Klein, Nigel, Coll Figa, Pedro|||0000-0003-1782-9782, Harris, Kathryn A.
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:226508
Acceso en línea:https://ddd.uab.cat/record/226508
https://dx.doi.org/urn:doi:10.1093/cid/ciz526
Access Level:acceso abierto
Palabra clave:Nontuberculous mycobacteria
Whole-genome sequencing
Transmission
Cystic fibrosis
Phylogenomics
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spelling Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis PatientsDoyle, Ronan M.|||0000-0001-5001-1945Rubio, Marc|||0000-0002-4811-0642Dixon, Garth|||0000-0001-8165-3094Hartley, JohnKlein, NigelColl Figa, Pedro|||0000-0003-1782-9782Harris, Kathryn A.Nontuberculous mycobacteriaWhole-genome sequencingTransmissionCystic fibrosisPhylogenomicsMycobacterium abscessus is an extensively drug-resistant pathogen that causes pulmonary disease, particularly in cystic fibrosis (CF) patients. Identifying direct patient-to-patient transmission of M. abscessus is critically important in directing an infection control policy for the management of risk in CF patients. A variety of clinical labs have used molecular epidemiology to investigate transmission. However, there is still conflicting evidence as to how M. abscessus is acquired and whether cross-transmission occurs. Recently, labs have applied whole-genome sequencing (WGS) to investigate this further and, in this study, we investigated whether WGS can reliably identify cross-transmission in M. abscessus. We retrospectively sequenced the whole genomes of 145 M. abscessus isolates from 62 patients, seen at 4 hospitals in 2 countries over 16 years. We have shown that a comparison of a fixed number of core single nucleotide variants alone cannot be used to infer cross-transmission in M. abscessus but does provide enough information to replace multiple existing molecular assays. We detected 1 episode of possible direct patient-to-patient transmission in a sibling pair. We found that patients acquired unique M. abscessus strains even after spending considerable time on the same wards with other M. abscessus- positive patients. This novel analysis has demonstrated that the majority of patients in this study have not acquired M. abscessus through direct patient-to-patient transmission or a common reservoir. Tracking transmission using WGS will only realize its full potential with proper environmental screening, as well as patient sampling. Whole-genome sequencing should replace the current molecular typing used routinely in clinical microbiology laboratories. The patient-to-patient spread of Mycobacterium abscessus is not common. Environmental screening may provide a better understanding of the acquisition of M. abscessus infections. 22019-01-0120192019-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/226508https://dx.doi.org/urn:doi:10.1093/cid/ciz526reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2265082026-06-06T12:50:31Z
dc.title.none.fl_str_mv Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients
title Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients
spellingShingle Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients
Doyle, Ronan M.|||0000-0001-5001-1945
Nontuberculous mycobacteria
Whole-genome sequencing
Transmission
Cystic fibrosis
Phylogenomics
title_short Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients
title_full Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients
title_fullStr Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients
title_full_unstemmed Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients
title_sort Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients
dc.creator.none.fl_str_mv Doyle, Ronan M.|||0000-0001-5001-1945
Rubio, Marc|||0000-0002-4811-0642
Dixon, Garth|||0000-0001-8165-3094
Hartley, John
Klein, Nigel
Coll Figa, Pedro|||0000-0003-1782-9782
Harris, Kathryn A.
author Doyle, Ronan M.|||0000-0001-5001-1945
author_facet Doyle, Ronan M.|||0000-0001-5001-1945
Rubio, Marc|||0000-0002-4811-0642
Dixon, Garth|||0000-0001-8165-3094
Hartley, John
Klein, Nigel
Coll Figa, Pedro|||0000-0003-1782-9782
Harris, Kathryn A.
author_role author
author2 Rubio, Marc|||0000-0002-4811-0642
Dixon, Garth|||0000-0001-8165-3094
Hartley, John
Klein, Nigel
Coll Figa, Pedro|||0000-0003-1782-9782
Harris, Kathryn A.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Nontuberculous mycobacteria
Whole-genome sequencing
Transmission
Cystic fibrosis
Phylogenomics
topic Nontuberculous mycobacteria
Whole-genome sequencing
Transmission
Cystic fibrosis
Phylogenomics
description Mycobacterium abscessus is an extensively drug-resistant pathogen that causes pulmonary disease, particularly in cystic fibrosis (CF) patients. Identifying direct patient-to-patient transmission of M. abscessus is critically important in directing an infection control policy for the management of risk in CF patients. A variety of clinical labs have used molecular epidemiology to investigate transmission. However, there is still conflicting evidence as to how M. abscessus is acquired and whether cross-transmission occurs. Recently, labs have applied whole-genome sequencing (WGS) to investigate this further and, in this study, we investigated whether WGS can reliably identify cross-transmission in M. abscessus. We retrospectively sequenced the whole genomes of 145 M. abscessus isolates from 62 patients, seen at 4 hospitals in 2 countries over 16 years. We have shown that a comparison of a fixed number of core single nucleotide variants alone cannot be used to infer cross-transmission in M. abscessus but does provide enough information to replace multiple existing molecular assays. We detected 1 episode of possible direct patient-to-patient transmission in a sibling pair. We found that patients acquired unique M. abscessus strains even after spending considerable time on the same wards with other M. abscessus- positive patients. This novel analysis has demonstrated that the majority of patients in this study have not acquired M. abscessus through direct patient-to-patient transmission or a common reservoir. Tracking transmission using WGS will only realize its full potential with proper environmental screening, as well as patient sampling. Whole-genome sequencing should replace the current molecular typing used routinely in clinical microbiology laboratories. The patient-to-patient spread of Mycobacterium abscessus is not common. Environmental screening may provide a better understanding of the acquisition of M. abscessus infections.
publishDate 2019
dc.date.none.fl_str_mv 2
2019-01-01
2019
2019-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/226508
https://dx.doi.org/urn:doi:10.1093/cid/ciz526
url https://ddd.uab.cat/record/226508
https://dx.doi.org/urn:doi:10.1093/cid/ciz526
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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